A 13 bp palindrome is a functional estrogen responsive element and interacts specifically with estrogen receptor
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This work has defined the 13 bp palindrome GGTCACAGTGACC as a minimal functional estrogen responsive element (ERE), which binds estrogen receptor preferentially in vitro and point mutations within the ERE decrease its affinity for the estrogen receptor and result in a complete loss of estrogen inducibility.Abstract:
Sequences located upstream of the transcription initiation site of the Xenopus vitellogenin A2 (vit A2) gene contain a hormone dependent enhancer that confers estrogen control to the heterologous thymidine kinase (tk) promoter. As a minimal functional estrogen responsive element (ERE), we have defined the 13 bp palindrome GGTCACAGTGACC. This ERE binds estrogen receptor preferentially in vitro. Although the ERE shares some structural features with the glucocorticoid responsive element (GRE) it is distinct from this element since it neither binds glucocorticoid receptor in vitro nor does it confer glucocorticoid inducibility to a fusion gene. Point mutations within the ERE decrease its affinity for the estrogen receptor and result in a complete loss of estrogen inducibility.read more
Citations
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Journal ArticleDOI
Estrogen response module of the mouse lactoferrin gene contains overlapping chicken ovalbumin upstream promoter transcription factor and estrogen receptor-binding elements.
Ying Liu,Christina T. Teng +1 more
TL;DR: It is found that the COUP/ERE element confers estrogen action to both homologous and heterologous promoters, and is named mERM, the mouse lactoferrin estrogen response module.
Journal ArticleDOI
The oestrogen receptor recognizes an imperfectly palindromic response element through an alternative side-chain conformation
TL;DR: This study suggests that proteins adapt to recognize different DNA sequences by rearranging side chains at the protein-DNA interface so as to form alternative patterns of intermolecular contacts.
Journal ArticleDOI
Estrogen regulation in human breast cancer cells of new downstream gene targets involved in estrogen metabolism, cell proliferation and cell transformation.
Julie A. Vendrell,Fabrice Magnino,E Danis,M J Duchesne,S Pinloche,M. Pons,David Jérémie Birnbaum,C Nguyen,Charles Theillet,Pascale A. Cohen +9 more
TL;DR: These findings should enhance the understanding of changes induced by E(2) on the transcriptional program of human E( 2)-responsive cells and permit the identification of new potential diagnostic/prognostic tools for the monitoring of estrogen-related disease conditions such as breast cancer.
Journal ArticleDOI
The protooncogene c-jun contains an unusual estrogen-inducible enhancer within the coding sequence.
TL;DR: A model in which the estrogen receptor functions as a heterodimer to regulate transcription of the c- jun protooncogene is suggested, which support a role for AP-1 components as early response genes in estrogen-induced proliferation.
Journal ArticleDOI
Use of reporter cell lines for detection of endocrine-disrupter activity
Philippe Willemsen,Marie-Louise Scippo,G. Kausel,Jaime Figueroa,Guy Maghuin-Rogister,Joseph Martial,Marc Muller +6 more
TL;DR: It is shown that the phytoestrogens apigenin and genistin have progestagenic and androgenic activity, respectively and the use of this high-throughput, cell-based assay for analysis of steroid (ant)agonists is reported.
References
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