A 13 bp palindrome is a functional estrogen responsive element and interacts specifically with estrogen receptor
Reads0
Chats0
TLDR
This work has defined the 13 bp palindrome GGTCACAGTGACC as a minimal functional estrogen responsive element (ERE), which binds estrogen receptor preferentially in vitro and point mutations within the ERE decrease its affinity for the estrogen receptor and result in a complete loss of estrogen inducibility.Abstract:
Sequences located upstream of the transcription initiation site of the Xenopus vitellogenin A2 (vit A2) gene contain a hormone dependent enhancer that confers estrogen control to the heterologous thymidine kinase (tk) promoter. As a minimal functional estrogen responsive element (ERE), we have defined the 13 bp palindrome GGTCACAGTGACC. This ERE binds estrogen receptor preferentially in vitro. Although the ERE shares some structural features with the glucocorticoid responsive element (GRE) it is distinct from this element since it neither binds glucocorticoid receptor in vitro nor does it confer glucocorticoid inducibility to a fusion gene. Point mutations within the ERE decrease its affinity for the estrogen receptor and result in a complete loss of estrogen inducibility.read more
Citations
More filters
Journal ArticleDOI
Specific binding of estrogen receptor to the estrogen response element.
TL;DR: It is reported that binding of the estrogen receptor to the ERE can be detected by a gel retardation (band shift) assay, and methylation interference analysis showed that the Ere contact sites of estrogen receptor displayed a perfect twofold rotational symmetry.
Journal ArticleDOI
Phospholipid-hydroperoxide glutathione peroxidase : genomic DNA, cDNA, and deduced amino acid sequence
Regina Brigelius-Flohé,K D Aumann,H Blöcker,G Gross,Michael Kiess,K D Klöppel,Matilde Maiorino,Antonella Roveri,R Schuckelt,F Usani +9 more
TL;DR: The complete amino acid sequence of the selenoprotein phospholipid-hydroperoxide glutathione peroxidase (PHGPX) from pig heart has been deduced from the corresponding genomic DNA, the cDNA covering the coding region, and by sequencing the N terminus of the protein.
Journal ArticleDOI
Estrogen response element-dependent regulation of transcriptional activation of estrogen receptors α and β by coactivators and corepressors
TL;DR: Testing the hypothesis that alterations in ER conformation induced by binding to different ERE sequences modulates ER interaction with coactivators and corepressors demonstrated that the ERE sequence impacts estradiol-and 4-hydroxytamoxifen-occupied ERalpha and ERbeta interaction with coregulators as measured by transcriptional activity in mammalian cells.
Journal ArticleDOI
Targeting steroid hormone receptors for ubiquitination and degradation in breast and prostate cancer.
Agustin Rodriguez-Gonzalez,Kedra Cyrus,Michael J. Salcius,Kyung Bo Kim,Craig M. Crews,Raymond J. Deshaies,Raymond J. Deshaies,Kathleen M. Sakamoto +7 more
TL;DR: The results suggest that Protacs specifically inhibit the proliferation of hormone-dependent breast and prostate cancer cells through degradation of the ERα and AR, respectively.
Journal ArticleDOI
The connexin43 gene is responsive to oestrogen.
TL;DR: Molecular cloning of theconnexin43 gene reveals a similar organization to that exhibited by other connexin genes: the 5' untranslated region is interrupted by an 8.5 kilobase intron and the promoter region preceding the first exon contains a TATA box and AP-1 andAP-2 sites.
References
More filters
Book
Gene Regulation by Steroid Hormones II
Arun K. Roy,James H. Clark +1 more
TL;DR: The authors discuss the latest advances in molecular endocrinology: - steroid receptor binding to DNA sequences of hormonally controlled genes, - structure of genes controlled by steroid hormones, - heterogeneity of steroid receptors, - immunochemical approaches to receptor studies, and - the most recent approaches to steroid hormone action and biological response.
Book
Molecular biology of egg maturation
Ruth Porter,Julie Whelan +1 more
TL;DR: Do you know the authors' friends become fans of molecular biology of egg maturation as the best book to read?