A 13 bp palindrome is a functional estrogen responsive element and interacts specifically with estrogen receptor
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This work has defined the 13 bp palindrome GGTCACAGTGACC as a minimal functional estrogen responsive element (ERE), which binds estrogen receptor preferentially in vitro and point mutations within the ERE decrease its affinity for the estrogen receptor and result in a complete loss of estrogen inducibility.Abstract:
Sequences located upstream of the transcription initiation site of the Xenopus vitellogenin A2 (vit A2) gene contain a hormone dependent enhancer that confers estrogen control to the heterologous thymidine kinase (tk) promoter. As a minimal functional estrogen responsive element (ERE), we have defined the 13 bp palindrome GGTCACAGTGACC. This ERE binds estrogen receptor preferentially in vitro. Although the ERE shares some structural features with the glucocorticoid responsive element (GRE) it is distinct from this element since it neither binds glucocorticoid receptor in vitro nor does it confer glucocorticoid inducibility to a fusion gene. Point mutations within the ERE decrease its affinity for the estrogen receptor and result in a complete loss of estrogen inducibility.read more
Citations
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Journal ArticleDOI
Aprotinin inhibits the hormone binding of the estrogen receptor from calf uterus.
Vincenzo Nigro,Nicola Medici,Ciro Abbondanza,Saverio Minucci,Anna Maria Molinari,Giovanni Alfredo Puca +5 more
TL;DR: Micromolar concentrations of the proteinase inhibitor, aprotinin, produced a dose-dependent inhibition in the binding capacity of the estrogen receptor from calf uterus and the bindingcapacity of the highly purified estrogen-binding subunit was similarly inhibited.
Book ChapterDOI
Molecular, Cellular, and Systemic Mechanisms of Antiestrogen Action
TL;DR: By studying this effective cancer therapeutic, investigators will develop a better understanding of breast carcinogenesis, which may translate to superior treatment regimens.
Estrogen Receptor Beta and p53 Play Integral Roles in Estradiol Mediated Protection against Colon Tumor Development
TL;DR: It is demonstrated that young adult mouse colonocytes (YAMC, nonmalignant colonocytes) exhibit an anti-proliferative response to E2 treatment, and isogenic YAMC cell lines with and without a dominant negative p53 mutation are utilized to demonstrate that this E2 induced action involves p53 activity.
Dissertation
Modification of oestrogen signalling during the acquisition of hormone resistance in breast cancer cells
TL;DR: Investigation of changes in endocrine response and ER transcription activation during the acquisition of endocrine resistance in breast cancer cell lines suggests ERa functionality in these cell lines.
Journal ArticleDOI
Lack of excision of introns from primary transcripts of certain chicken vitellogenin II minigenes.
TL;DR: These experiments constitute the first reported attempts to express authentic VTGII coding sequences in cultured cells and highlight the dilemma of which introns to include in a minigene.
References
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Book
Gene Regulation by Steroid Hormones II
Arun K. Roy,James H. Clark +1 more
TL;DR: The authors discuss the latest advances in molecular endocrinology: - steroid receptor binding to DNA sequences of hormonally controlled genes, - structure of genes controlled by steroid hormones, - heterogeneity of steroid receptors, - immunochemical approaches to receptor studies, and - the most recent approaches to steroid hormone action and biological response.
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Molecular biology of egg maturation
Ruth Porter,Julie Whelan +1 more
TL;DR: Do you know the authors' friends become fans of molecular biology of egg maturation as the best book to read?