Assay Techniques and Test Development for COVID-19 Diagnosis
Linda J. Carter,Linda V. Garner,Jeffrey Smoot,Yingzhu Li,Qiongqiong Zhou,Catherine J. Saveson,Janet M. Sasso,Anne C. Gregg,Divya J. Soares,Tiffany R. Beskid,Susan R. Jervey,Cynthia Liu +11 more
Reads0
Chats0
TLDR
An ongoing theme of the COVID-19 pandemic is the need for widespread availability of accurate and efficient diagnostic testing for detection of SARS-CoV-2 and antiviral antibodies in infected indiv...Abstract:
An ongoing theme of the COVID-19 pandemic is the need for widespread availability of accurate and efficient diagnostic testing for detection of SARS-CoV-2 and antiviral antibodies in infected indiv...read more
Citations
More filters
Journal ArticleDOI
Rapid, point-of-care antigen and molecular-based tests for diagnosis of SARS-CoV-2 infection.
Jacqueline Dinnes,Jacqueline Dinnes,Jonathan J Deeks,Ada Adriano,Sarah Berhane,Clare Davenport,Sabine Dittrich,Devy Emperador,Yemisi Takwoingi,Jane Cunningham,Sophie Beese,Janine Dretzke,Lavinia Ferrante di Ruffano,Isobel Marion Harris,Malcolm J Price,Sian Taylor-Phillips,Lotty Hooft,Mariska M.G. Leeflang,René Spijker,Ann Van den Bruel +19 more
TL;DR: Assessment of the diagnostic accuracy of point‐of‐care antigen and molecular‐based tests to determine if a person presenting in the community or in primary or secondary care has current SARS‐CoV‐2 infection found no studies at low risk of bias for all quality domains and concerns about applicability of results across all studies.
Journal ArticleDOI
Selective Naked-Eye Detection of SARS-CoV-2 Mediated by N Gene Targeted Antisense Oligonucleotide Capped Plasmonic Nanoparticles.
Parikshit Moitra,Maha Alafeef,Maha Alafeef,Maha Alafeef,Ketan Dighe,Matthew B. Frieman,Dipanjan Pan,Dipanjan Pan,Dipanjan Pan +8 more
TL;DR: A colorimetric assay based on gold nanoparticles (AuNPs), when capped with suitably designed thiol-modified antisense oligonucleotides (ASOs) specific for N-gene (nucleocapsid phosphoprotein) of SARS-CoV-2, could be used for diagnosing positive COVID-19 cases within 10 min from the isolated RNA samples.
Journal ArticleDOI
The COVID-19 pandemic
Marco Ciotti,Massimo Ciccozzi,Alessandro Terrinoni,Wen Can Jiang,Cheng Bin Wang,Sergio Bernardini +5 more
TL;DR: The available therapies to fight CO VID-19, the development of vaccines, the role of artificial intelligence in the management of the pandemic and limiting the spread of the virus, the impact of the COVID-19 epidemic on the authors' lifestyle, and preparation for a possible second wave are provided.
Journal ArticleDOI
Systematic review with meta-analysis of the accuracy of diagnostic tests for COVID-19.
Beatriz Böger,Mariana Millan Fachi,Raquel de Oliveira Vilhena,Alexandre de Fátima Cobre,Fernanda S. Tonin,Roberto Pontarolo +5 more
TL;DR: RT-PCR remains the gold standard for the diagnosis of COVID-19 in sputum samples and the combination of different diagnostic tests is highly recommended to achieve adequate sensitivity and specificity.
Journal ArticleDOI
Rapid, Ultrasensitive, and Quantitative Detection of SARS-CoV-2 Using Antisense Oligonucleotides Directed Electrochemical Biosensor Chip.
Maha Alafeef,Maha Alafeef,Maha Alafeef,Ketan Dighe,Ketan Dighe,Parikshit Moitra,Dipanjan Pan,Dipanjan Pan,Dipanjan Pan +8 more
TL;DR: The development of a rapid (less than 5 min), low-cost, easy-to-implement, and quantitative paper-based electrochemical sensor chip to enable the digital detection of SARS-CoV-2 genetic material is reported.
References
More filters
Journal ArticleDOI
Loop-mediated isothermal amplification of DNA
Tsugunori Notomi,Hiroto Okayama,Harumi Otawara-shi Masubuchi,Toshihiro Yonekawa,Keiko Watanabe,Nobuyuki Amino,Tetsu Hase +6 more
TL;DR: A novel method that amplifies DNA with high specificity, efficiency and rapidity under isothermal conditions that employs a DNA polymerase and a set of four specially designed primers that recognize a total of six distinct sequences on the target DNA.
Journal ArticleDOI
SARS-CoV-2 Viral Load in Upper Respiratory Specimens of Infected Patients.
Lirong Zou,Feng Ruan,Mingxing Huang,Lijun Liang,Huitao Huang,Zhongsi Hong,Jianxiang Yu,Min Kang,Yingchao Song,Jinyu Xia,Qianfang Guo,Tie Song,Jianfeng He,Hui-Ling Yen,Malik Peiris,Jie Wu +15 more
TL;DR: Results of an analysis of nasal and throat swabs from 17 patients in Zhuhai, China, who had received a diagnosis of Covid-19 and found SARS-CoV-2 Viral Load in Upper Respiratory Specimens positive.
Journal ArticleDOI
Estimating the asymptomatic proportion of coronavirus disease 2019 (COVID-19) cases on board the Diamond Princess cruise ship, Yokohama, Japan, 2020.
Kenji Mizumoto,Kenji Mizumoto,Katsushi Kagaya,Katsushi Kagaya,Alexander E. Zarebski,Gerardo Chowell +5 more
TL;DR: On 5 February 2020, in Yokohama, Japan, a cruise ship hosting 3,711 people underwent a 2-week quarantine after a former passenger was found with COVID-19 post-disembarking, and the delay-adjusted asymptomatic proportion of infections, along with the infections’ timeline were derived.
Journal ArticleDOI
Real-time PCR for mRNA quantitation.
Marisa L. Wong,Juan F. Medrano +1 more
TL;DR: This review discusses the key components of a real-time PCR experiment, including one-step or two-step PCR, absolute versus relative quantitation, mathematical models available for Relative quantitation and amplification efficiency calculations, types of normalization or data correction, and detection chemistries.
Journal ArticleDOI
CRISPR-Cas12-based detection of SARS-CoV-2.
James Paul Broughton,Xianding Deng,Guixia Yu,Clare L Fasching,Venice Servellita,Jasmeet Singh,Xin Miao,Jessica Streithorst,Andrea Granados,Alicia Sotomayor-Gonzalez,Kelsey C. Zorn,Allan Gopez,Elaine Hsu,Wei Gu,Steve Miller,Chao Yang Pan,Hugo Guevara,Debra A. Wadford,Janice S. Chen,Charles Y. Chiu +19 more
TL;DR: The CRISPR-based DETECTR assay provides a visual and faster alternative to the US Centers for Disease Control and Prevention SARS-CoV-2 real-time RT–PCR assay, with 95% positive predictive agreement and 100% negative predictive agreement.
Related Papers (5)
Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR.
Victor M. Corman,Olfert Landt,Marco Kaiser,Richard Molenkamp,Adam Meijer,Daniel K.W. Chu,Tobias Bleicker,Sebastian Brünink,Julia Schneider,Marie Luisa Schmidt,Daphne G.J.C. Mulders,Bart L. Haagmans,Bas van der Veer,Sharon van den Brink,Lisa Wijsman,Gabriel Goderski,Jean Louis Romette,Joanna Ellis,Maria Zambon,Malik Peiris,Herman Goossens,Chantal B.E.M. Reusken,Marion Koopmans,Christian Drosten +23 more
Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China
Chaolin Huang,Yeming Wang,Xingwang Li,Lili Ren,Jianping Zhao,Yi Hu,Li Zhang,Guohui Fan,Jiuyang Xu,Xiaoying Gu,Zhenshun Cheng,Ting Yu,Jia'an Xia,Yuan Wei,Wenjuan Wu,Xuelei Xie,Wen Yin,Li Hui,Min Liu,Yan Xiao,Hong Gao,Li Guo,Jungang Xie,Guang-Fa Wang,Rongmeng Jiang,Zhancheng Gao,Qi Jin,Jianwei Wang,Bin Cao +28 more
A pneumonia outbreak associated with a new coronavirus of probable bat origin
Peng Zhou,Xing-Lou Yang,Xian Guang Wang,Ben Hu,Lei Zhang,Wei Zhang,Hao Rui Si,Yan Zhu,Bei Li,Chao Lin Huang,Hui-Dong Chen,Jing Chen,Yun Luo,Hua Guo,Ren Di Jiang,Meiqin Liu,Ying Chen,Xu Rui Shen,Xi Wang,Xiao Shuang Zheng,Kai Zhao,Quanjiao Chen,Fei Deng,Lin Lin Liu,Bing Yan,Fa Xian Zhan,Yan-Yi Wang,Gengfu Xiao,Zhengli Shi +28 more