Journal ArticleDOI
Bone marrow as a potential source of hepatic oval cells.
Bryon E. Petersen,W. C. Bowen,K. D. Patrene,Wendy M. Mars,A. K. Sullivan,N. Murase,S. S. Boggs,Joel S. Greenberger,Julie P. Goff +8 more
TLDR
A stem cell associated with the bone marrow has epithelial cell lineage capability and a proportion of the regenerated hepatic cells were shown to be donor-derived.Abstract:
Bone marrow stem cells develop into hematopoietic and mesenchymal lineages but have not been known to participate in production of hepatocytes, biliary cells, or oval cells during liver regeneration. Cross-sex or cross-strain bone marrow and whole liver transplantation were used to trace the origin of the repopulating liver cells. Transplanted rats were treated with 2-acetylaminofluorene, to block hepatocyte proliferation, and then hepatic injury, to induce oval cell proliferation. Markers for Y chromosome, dipeptidyl peptidase IV enzyme, and L21-6 antigen were used to identify liver cells of bone marrow origin. From these cells, a proportion of the regenerated hepatic cells were shown to be donor-derived. Thus, a stem cell associated with the bone marrow has epithelial cell lineage capability.read more
Citations
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Journal ArticleDOI
Gene repair and transposon-mediated gene therapy.
TL;DR: The main strategy of gene therapy has traditionally been focused on gene augmentation, but the process involves targeting the mutation in situ for gene correction and a return to normal gene function.
Journal ArticleDOI
Comparison of neuron-like cells derived from bone marrow stem cells to those differentiated from adult brain neural stem cells.
TL;DR: Nestin-expressing bone marrow and the adult brain neural stem/progenitors developed into cells with morphological, immunocytochemical, and functional characteristics of neurons, which may be useful in the cellular therapy of neurodegenerative diseases and traumatic brain and spinal cord injury.
Journal ArticleDOI
Bone marrow-derived stem cells and "plasticity".
TL;DR: The role of bone marrow-derived stem cells in the field of experimental and clinical plasticity studies is discussed and Observations compatible with the concept of stem cell plasticity will be weighed against limitations of the experimental systems employed.
Journal ArticleDOI
Quantitative Gene Expression Analysis Reveals Transition of Fetal Liver Progenitor Cells to Mature Hepatocytes after Transplantation in uPA/RAG-2 Mice
Tobias Cantz,David M. Zuckerman,Martin R. Burda,Maura Dandri,Bettina Göricke,Stefan Thalhammer,Wolfgang M. Heckl,Michael P. Manns,Jörg Petersen,Michael Ott +9 more
TL;DR: It is demonstrated that gene expression in liver progenitor cells changes from fetal to adult phenotype within 4 to 6 weeks after transplantation despite ongoing proliferation of the transplanted cells in a mouse model of liver regeneration.
Journal ArticleDOI
Stem cells and regenerative medicine
TL;DR: Stem cells have been defined as clonogenic cells that undergo both self-renewal and differentiation to more committed progenitors and functionally specialized mature cells.
References
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Journal ArticleDOI
Muscle Regeneration by Bone Marrow-Derived Myogenic Progenitors
Giuliana Ferrari,Gabriella Cusella,Gabriella Cusella,D. De Angelis,D. De Angelis,M. Coletta,M. Coletta,Egle Paolucci,Egle Paolucci,Anna Stornaiuolo,Anna Stornaiuolo,Giulio Cossu,Giulio Cossu,Fulvio Mavilio,Fulvio Mavilio +14 more
TL;DR: Transplantation of genetically marked bone marrow into immunodeficient mice revealed that marrow-derived cells migrate into areas of induced muscle degeneration, undergo myogenic differentiation, and participate in the regeneration of the damaged fibers.
Journal Article
In vivo differentiation of rat liver oval cells into hepatocytes.
Ritva P. Evarts,Peter Nagy,Harushige Nakatsukasa,Elizabeth R. Marsden,Snorri S. Thorgeirsson +4 more
TL;DR: The data indicate that oval cells can differentiate to hepatocytes and may have an important physiological function as a source of major serum proteins when hepatocytes are unable to synthesize these proteins.
Journal ArticleDOI
Presence of hematopoietic stem cells in the adult liver
TL;DR: The data obtained from the mouse study strongly suggest that hematopoietic stem cells residing in the donor liver are responsible for mixed chimerism and maintenance of tolerance after liver transplantation.
Journal Article
Expression of stem cell factor and its receptor, c-kit, during liver regeneration from putative stem cells in adult rat.
TL;DR: The SCF/c-kit system may, possibly in combination with other growth factor/receptor systems, be involved in the early activation of the hepatic stem cells as well as in the expansion and differentiation of oval cells.
Journal ArticleDOI
Variable chimerism, graft-versus-host disease, and tolerance after different kinds of cell and whole organ transplantation from Lewis to brown Norway rats.
Noriko Murase,Thomas E. Starzl,Minoru Tanabe,Shigeru Fujisaki,H. Miyazawa,Qing Ye,Conor P. Delaney,John J. Fung,Anthony J. Demetris +8 more
TL;DR: Both the GVHD propensity and tolerogenicity in these experiments were closely associated with recipient tissue chimerism 30 and 100 days after the experiments began, and these observations provide guidelines that should be considered in devising leukocyte augmentation protocols for human whole organ recipients.