Journal ArticleDOI
Bone marrow as a potential source of hepatic oval cells.
Bryon E. Petersen,W. C. Bowen,K. D. Patrene,Wendy M. Mars,A. K. Sullivan,N. Murase,S. S. Boggs,Joel S. Greenberger,Julie P. Goff +8 more
TLDR
A stem cell associated with the bone marrow has epithelial cell lineage capability and a proportion of the regenerated hepatic cells were shown to be donor-derived.Abstract:
Bone marrow stem cells develop into hematopoietic and mesenchymal lineages but have not been known to participate in production of hepatocytes, biliary cells, or oval cells during liver regeneration. Cross-sex or cross-strain bone marrow and whole liver transplantation were used to trace the origin of the repopulating liver cells. Transplanted rats were treated with 2-acetylaminofluorene, to block hepatocyte proliferation, and then hepatic injury, to induce oval cell proliferation. Markers for Y chromosome, dipeptidyl peptidase IV enzyme, and L21-6 antigen were used to identify liver cells of bone marrow origin. From these cells, a proportion of the regenerated hepatic cells were shown to be donor-derived. Thus, a stem cell associated with the bone marrow has epithelial cell lineage capability.read more
Citations
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Journal ArticleDOI
Stem cells today: B1. Bone marrow stem cells.
TL;DR: This review covers bone marrow stem cells and describes methods of identifying, culturing, expanding and grafting stem cells, including the separation of haemopoietic and mesenchyme cell lines (HSC and MSC) and recent more detailed analyses.
Journal ArticleDOI
Progress in stem cell-based therapy for liver disease.
Goshi Shiota,Noriko Itaba +1 more
TL;DR: All reports on clinical trials of cell therapy for liver disease that have been recently undertaken using mesenchymal stem cells, hematopoieticstem cells, bone marrow mononuclear cells, and liver progenitor cells are reviewed to provide a perspective on the available approaches based on stem cell‐based therapy for Liver disease.
Patent
Method for transdifferentiation of non-pancreatic stem cells to the pancreatic pathway
TL;DR: In this paper, a method of transdifferentiating mammalian non-pancreatic stem cells, such as mesenchymal stem cells (MSCs), to enter the pancreatic differentiations pathway was proposed.
Journal ArticleDOI
Analysis of neurons created from wild-type and Alzheimer's mutation knock-in embryonic stem cells by a highly efficient differentiation protocol
Yoichiro Abe,Keisuke Kouyama,Taisuke Tomita,Yusuke Tomita,Norimitsu Ban,Mikiro Nawa,Masaaki Matsuoka,Takako Niikura,Sadakazu Aiso,Yoshiko Kita,Takeshi Iwatsubo,Ikuo Nishimoto +11 more
TL;DR: This study constructed mouse embryonic stem cells, in which the AD-causative V642I mutation was introduced to the endogenous amyloid precursor protein (APP) gene, in combination with a protocol to efficiently differentiate ES cells into postmitotic neurons without using a cell sorter, to establish the neurons harboring AD abnormality.
Journal ArticleDOI
Differentiation of hematopoietic stem cells into hepatocytes in liver fibrosis in rats.
TL;DR: Investigation of whether hematopoietic stem cells can differentiate into hepatocytes in cases of established liver fibrosis found that autologous stem cell transplantation may be helpful to treat hepatic fibrosis.
References
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Journal ArticleDOI
Muscle Regeneration by Bone Marrow-Derived Myogenic Progenitors
Giuliana Ferrari,Gabriella Cusella,Gabriella Cusella,D. De Angelis,D. De Angelis,M. Coletta,M. Coletta,Egle Paolucci,Egle Paolucci,Anna Stornaiuolo,Anna Stornaiuolo,Giulio Cossu,Giulio Cossu,Fulvio Mavilio,Fulvio Mavilio +14 more
TL;DR: Transplantation of genetically marked bone marrow into immunodeficient mice revealed that marrow-derived cells migrate into areas of induced muscle degeneration, undergo myogenic differentiation, and participate in the regeneration of the damaged fibers.
Journal Article
In vivo differentiation of rat liver oval cells into hepatocytes.
Ritva P. Evarts,Peter Nagy,Harushige Nakatsukasa,Elizabeth R. Marsden,Snorri S. Thorgeirsson +4 more
TL;DR: The data indicate that oval cells can differentiate to hepatocytes and may have an important physiological function as a source of major serum proteins when hepatocytes are unable to synthesize these proteins.
Journal ArticleDOI
Presence of hematopoietic stem cells in the adult liver
TL;DR: The data obtained from the mouse study strongly suggest that hematopoietic stem cells residing in the donor liver are responsible for mixed chimerism and maintenance of tolerance after liver transplantation.
Journal Article
Expression of stem cell factor and its receptor, c-kit, during liver regeneration from putative stem cells in adult rat.
TL;DR: The SCF/c-kit system may, possibly in combination with other growth factor/receptor systems, be involved in the early activation of the hepatic stem cells as well as in the expansion and differentiation of oval cells.
Journal ArticleDOI
Variable chimerism, graft-versus-host disease, and tolerance after different kinds of cell and whole organ transplantation from Lewis to brown Norway rats.
Noriko Murase,Thomas E. Starzl,Minoru Tanabe,Shigeru Fujisaki,H. Miyazawa,Qing Ye,Conor P. Delaney,John J. Fung,Anthony J. Demetris +8 more
TL;DR: Both the GVHD propensity and tolerogenicity in these experiments were closely associated with recipient tissue chimerism 30 and 100 days after the experiments began, and these observations provide guidelines that should be considered in devising leukocyte augmentation protocols for human whole organ recipients.