Journal ArticleDOI
Bone marrow as a potential source of hepatic oval cells.
Bryon E. Petersen,W. C. Bowen,K. D. Patrene,Wendy M. Mars,A. K. Sullivan,N. Murase,S. S. Boggs,Joel S. Greenberger,Julie P. Goff +8 more
TLDR
A stem cell associated with the bone marrow has epithelial cell lineage capability and a proportion of the regenerated hepatic cells were shown to be donor-derived.Abstract:
Bone marrow stem cells develop into hematopoietic and mesenchymal lineages but have not been known to participate in production of hepatocytes, biliary cells, or oval cells during liver regeneration. Cross-sex or cross-strain bone marrow and whole liver transplantation were used to trace the origin of the repopulating liver cells. Transplanted rats were treated with 2-acetylaminofluorene, to block hepatocyte proliferation, and then hepatic injury, to induce oval cell proliferation. Markers for Y chromosome, dipeptidyl peptidase IV enzyme, and L21-6 antigen were used to identify liver cells of bone marrow origin. From these cells, a proportion of the regenerated hepatic cells were shown to be donor-derived. Thus, a stem cell associated with the bone marrow has epithelial cell lineage capability.read more
Citations
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Tissue engineering: challenges and opportunities.
TL;DR: The article outlines the challenges to the commercialization of tissue-engineered products, and highlights the ongoing efforts by the American Society for Testing and Materials (ASTM) in developing standards for tissue-Engineered medical products.
Journal ArticleDOI
Homeostatic regulation of germinal stem cell proliferation by the GDNF/FSH pathway
TL;DR: In mammalian testes, GSC proliferation and population size are regulated homeostatically by the GDNF/FSH pathway, and it is found that follicle-stimulating hormone (FSH) stimulation via homeostatic control was one of the major regulators of GDNF concentration.
Journal ArticleDOI
Isolation and characterization of size-sieved stem cells from human bone marrow
Shih-Chieh Hung,Shih-Chieh Hung,Nien Jung Chen,Shie-Liang Hsieh,Hung Li,Hsiao Li Ma,Hsiao Li Ma,Wai Hee Lo +7 more
TL;DR: A culture device is used to sieve out a homogeneous population of cells (termed size‐sieved [SS] cells) from bone marrow aspirates that had the capacity for self‐renewal and the multilineage potential to form bone, fat, and cartilage, and satisfy the characteristics of MSCs.
Journal ArticleDOI
Flow-cytometric separation and enrichment of hepatic progenitor cells in the developing mouse liver
Atsushi Suzuki,Yun Wen Zheng,Reika Kondo,Moriaki Kusakabe,Yasutsugu Takada,Katashi Fukao,Hiromitsu Nakauchi,Hideki Taniguchi +7 more
TL;DR: It is demonstrated that hepatic progenitor cells are enriched by FACS and suggested approaches to supplanting organ allografting and improving artificial‐organ hepatic support are suggested.
References
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Journal ArticleDOI
Muscle Regeneration by Bone Marrow-Derived Myogenic Progenitors
Giuliana Ferrari,Gabriella Cusella,Gabriella Cusella,D. De Angelis,D. De Angelis,M. Coletta,M. Coletta,Egle Paolucci,Egle Paolucci,Anna Stornaiuolo,Anna Stornaiuolo,Giulio Cossu,Giulio Cossu,Fulvio Mavilio,Fulvio Mavilio +14 more
TL;DR: Transplantation of genetically marked bone marrow into immunodeficient mice revealed that marrow-derived cells migrate into areas of induced muscle degeneration, undergo myogenic differentiation, and participate in the regeneration of the damaged fibers.
Journal Article
In vivo differentiation of rat liver oval cells into hepatocytes.
Ritva P. Evarts,Peter Nagy,Harushige Nakatsukasa,Elizabeth R. Marsden,Snorri S. Thorgeirsson +4 more
TL;DR: The data indicate that oval cells can differentiate to hepatocytes and may have an important physiological function as a source of major serum proteins when hepatocytes are unable to synthesize these proteins.
Journal ArticleDOI
Presence of hematopoietic stem cells in the adult liver
TL;DR: The data obtained from the mouse study strongly suggest that hematopoietic stem cells residing in the donor liver are responsible for mixed chimerism and maintenance of tolerance after liver transplantation.
Journal Article
Expression of stem cell factor and its receptor, c-kit, during liver regeneration from putative stem cells in adult rat.
TL;DR: The SCF/c-kit system may, possibly in combination with other growth factor/receptor systems, be involved in the early activation of the hepatic stem cells as well as in the expansion and differentiation of oval cells.
Journal ArticleDOI
Variable chimerism, graft-versus-host disease, and tolerance after different kinds of cell and whole organ transplantation from Lewis to brown Norway rats.
Noriko Murase,Thomas E. Starzl,Minoru Tanabe,Shigeru Fujisaki,H. Miyazawa,Qing Ye,Conor P. Delaney,John J. Fung,Anthony J. Demetris +8 more
TL;DR: Both the GVHD propensity and tolerogenicity in these experiments were closely associated with recipient tissue chimerism 30 and 100 days after the experiments began, and these observations provide guidelines that should be considered in devising leukocyte augmentation protocols for human whole organ recipients.