Conserved nucleosome positioning defines replication origins
TLDR
Using high-throughput sequencing to map ORC binding and nucleosome positioning, it is shown that yeast origins are characterized by an asymmetric pattern of positioned nucleosomes flanking the ACS.Abstract:
The origin recognition complex (ORC) specifies replication origin location. The Saccharomyces cerevisiae ORC recognizes the ARS (autonomously replicating sequence) consensus sequence (ACS), but only a subset of potential genomic sites are bound, suggesting other chromosomal features influence ORC binding. Using high-throughput sequencing to map ORC binding and nucleosome positioning, we show that yeast origins are characterized by an asymmetric pattern of positioned nucleosomes flanking the ACS. The origin sequences are sufficient to maintain a nucleosome-free origin; however, ORC is required for the precise positioning of nucleosomes flanking the origin. These findings identify local nucleosomes as an important determinant for origin selection and function.read more
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The Genomic Code for Nucleosome Positioning
TL;DR: In this article, a nucleosome-DNA interaction model was proposed to predict the genome-wide organization of nucleosomes, and it was shown that genomes encode an intrinsic nucleosomal organization and that this intrinsic organization can explain ∼50% of the in-vivo positions.
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TL;DR: The Drosophila Encyclopedia of DNA Elements (modENCODE) project as mentioned in this paper has been used to map transcripts, histone modifications, chromosomal proteins, transcription factors, replication proteins and intermediates, and nucleosome properties across a developmental time course and in multiple cell lines.
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References
More filters
Journal Article
The Genomic Code for Nucleosome Positioning
TL;DR: In this article, a nucleosome-DNA interaction model was proposed to predict the genome-wide organization of nucleosomes, and it was shown that genomes encode an intrinsic nucleosomal organization and that this intrinsic organization can explain ∼50% of the in-vivo positions.
Journal ArticleDOI
A genomic code for nucleosome positioning
Eran Segal,Yvonne N. Fondufe-Mittendorf,Lingyi Chen,Annchristine Thåström,Yair Field,Irene K. Moore,Ji Ping Wang,Jonathan Widom +7 more
TL;DR: This work isolated nucleosome-bound sequences at high resolution from yeast and used these sequences in a new computational approach to construct and validate experimentally a nucleosom–DNA interaction model, and to predict the genome-wide organization of nucleosomes.
Journal ArticleDOI
ATP-dependent recognition of eukaryotic origins of DNA replication by a multiprotein complex.
Stephen P. Bell,Bruce Stillman +1 more
TL;DR: It is proposed that the origin recognition complex acts as the initiator protein for S. cerevisiae origins of DNA replication, and specific DNA binding by theorigin recognition complex is dependent upon the addition of ATP.
Journal ArticleDOI
The DNA-encoded nucleosome organization of a eukaryotic genome
Noam Kaplan,Irene K. Moore,Yvonne N. Fondufe-Mittendorf,Andrea J. Gossett,Desiree Tillo,Yair Field,Emily M LeProust,Timothy P. Hughes,Jason D. Lieb,Jonathan Widom,Eran Segal +10 more
TL;DR: The results indicate that the intrinsic DNA sequence preferences of nucleosomes have a central role in determining the organization ofucleosomes in vivo.
Journal ArticleDOI
Components and Dynamics of DNA Replication Complexes in S. cerevisiae: Redistribution of MCM Proteins and Cdc45p during S Phase
TL;DR: The results identify protein components of the pre-RC and a novel replication complex appearing at the G1/S transition (the RC), and suggest that after initiation MCM proteins and Cdc45p move with eukaryotic replication forks.
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