Detecting DNA and RNA and Differentiating Single-Nucleotide Variations via Field-Effect Transistors
Kevin M Cheung,John M. Abendroth,Nako Nakatsuka,Bowen Zhu,Yang Yang,Anne M. Andrews,Anne M. Andrews,Paul S. Weiss +7 more
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TLDR
The capability of ssDNA-FETs to detect complementary RNA sequences and to distinguish from RNA sequences with single nucleotide variations is illustrated.Abstract:
We detect short oligonucleotides and distinguish between sequences that differ by a single base, using label-free, electronic field-effect transistors (FETs). Our sensing platform utilizes ultrathin-film indium oxide FETs chemically functionalized with single-stranded DNA (ssDNA). The ssDNA-functionalized semiconducting channels in FETs detect fully complementary DNA sequences and differentiate these sequences from those having different types and locations of single base-pair mismatches. Changes in charge associated with surface-bound ssDNA vs double-stranded DNA (dsDNA) alter FET channel conductance to enable detection due to differences in DNA duplex stability. We illustrate the capability of ssDNA-FETs to detect complementary RNA sequences and to distinguish from RNA sequences with single nucleotide variations. The development and implementation of electronic biosensors that rapidly and sensitively detect and differentiate oligonucleotides present new opportunities in the fields of disease diagnostics and precision medicine.read more
Citations
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Direct SARS-CoV-2 Nucleic Acid Detection by Y-Shaped DNA Dual-Probe Transistor Assay.
Derong Kong,Xuejun Wang,Chenjian Gu,Mingquan Guo,Yao Wang,Zhaolin Ai,Shen Zhang,Yiheng Chen,Wentao Liu,Yungen Wu,Changhao Dai,Qianying Guo,Di Qu,Zhaoqin Zhu,Youhua Xie,Yunqi Liu,Yunqi Liu,Dacheng Wei +17 more
TL;DR: In this paper, a direct nucleic acid assay by using a graphene field effect transistor (g-FET) with Y-shaped DNA dual probes (Y-dual probes) was presented.
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Fluorescence-Detected Circular Dichroism of a Chiral Molecular Monolayer with Dielectric Metasurfaces.
Michelle L. Solomon,John M. Abendroth,Lisa V. Poulikakos,Lisa V. Poulikakos,Jack Hu,Jennifer A. Dionne +5 more
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Technology Roadmap for Flexible Sensors.
Yi Fei Luo,Mohammad Reza Abidian,Jong Hyun Ahn,Deji Akinwande,Anne M. Andrews,Markus Antonietti,Zhenan Bao,Magnus Berggren,Christopher Berkey,Christopher J. Bettinger,Jun Chen,Peng Fei Chen,Wenlong Cheng,Xu Cheng,Seon-Jin Choi,Alex Chortos,Canan Dagdeviren,Reinhold H. Dauskardt,Chong-an Di,Michael D. Dickey,Xiangfeng Duan,Antonio Facchetti,Zhiyong Fan,Yin Fang,Jianyou Feng,Xue Feng,Huajian Gao,Wei Gao,Xiwen Gong,Chuan Fei Guo,Xiao Ling Guo,Martin C. Hartel,Zihan He,John S. Ho,Youfan Hu,Qiyao Huang,Yu Huang,Fengwei Huo,M. M. Hussain,Ali Javey,Unyong Jeong,Chen Jiang,Xingyu Jiang,Jiheong Kang,Daniil Karnaushenko,Ali Khademhosseini,Dae-Hyeong Kim,Il-Doo Kim,Dmitry Kireev,Lingxuan Kong,Chengkuo Lee,Nae-Eung Lee,Pooi See Lee,Tae-Woo Lee,Fengyu Li,Jinxing Li,Cuiyuan Liang,Chwee Teck Lim,Yuanjing Lin,Darren J. Lipomi,Jia Liu,Kai Liu,Nan Liu,Ren Liu,Yuxin Liu,Yuxuan Liu,Zhiyuan Liu,Zhuangjian Liu,Xian Jun Loh,Nanshu Lu,Zhi-Mei Lv,Shlomo Magdassi,George G. Malliaras,Naoji Matsuhisa,Arokia Nathan,Simiao Niu,Jieming Pan,Changhyun Pang,Qibing Pei,Huisheng Peng,Dianpeng Qi,Huaying Ren,John A. Rogers,Aaron W. Rowe,Oliver G. Schmidt,Tsuyoshi Sekitani,Dae-Gyo Seo,Guozhen Shen,Xing Sheng,Qiongfeng Shi,Takao Someya,Yanlin Song,Eleni Stavrinidou,Meng Su,Xuemei Sun,Kuniharu Takei,Xiaoming Tao,Benjamin C. K. Tee,A. Thean,Tran Quang Trung,Changjin Wan,Huiliang Wang,Joseph Wang,Ming Wei Wang,Si-Xian Wang,Ting Wang,Zhong Lin Wang,Paul S. Weiss,Hanqi Wen,Sheng Xu,Tailin Xu,Hongping Yan,Xuzhou Yan,Hui Yang,Le Yang,Shuaijian Yang,Lan Yin,Cunjiang Yu,Gui-Yun Yu,Jing Yu,Shu-Hong Yu,Xinge Yu,E. G. Zamburg,Haixia Zhang,XiangYu Zhang,Xiao-Sheng Zhang,Xueji Zhang,Yihui Zhang,Yu Zhang,Siyuan Zhao,Xuanhe Zhao,Yuanjin Zheng,Yu-Qing Zheng,Zijian Zheng,Tao Zhou,Bowen Zhu,Ming Zhu,Rong Zhu,Yangzhi Zhu,Yong-Guan Zhu,Guijin Zou,Xiaodong Chen +141 more
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Narrower Nanoribbon Biosensors Fabricated by Chemical Lift-off Lithography Show Higher Sensitivity.
Chuanzhen Zhao,Qingzhou Liu,Kevin M Cheung,Wenfei Liu,Qing Yang,Xiaobin Xu,Tianxing Man,Paul S. Weiss,Chongwu Zhou,Anne M. Andrews,Anne M. Andrews +10 more
TL;DR: In this paper, a large-scale nanoribboned field effect transistor (FET) biosensors with high sensitivity to a broad range of biological targets are demonstrated.
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