PatentDOI
Direct reprogramming of human fibroblasts to functional neurons under defined conditions
Sheng Ding,Rajesh Ambasudhan +1 more
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TLDR
In this paper, the authors presented methods of generating a neuronal cell from a differentiated non-neuronal cell by increasing the amount of miR-124 microRNA, a MYT1L transcription factor, and a BRN2 transcription factor.About:
This article is published in Cell Stem Cell.The article was published on 2012-03-01. It has received 431 citations till now. The article focuses on the topics: Cellular differentiation & Transcription factor.read more
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Journal ArticleDOI
Super-Enhancers in the Control of Cell Identity and Disease
Denes Hnisz,Brian J. Abraham,Tong Ihn Lee,Ashley Lau,Violaine Saint-André,Alla A. Sigova,Heather A. Hoke,Richard A. Young +7 more
TL;DR: The super-enhancers are large clusters of transcriptional enhancers that drive expression of genes that define cell identity and play key roles in human cell identity in health and in disease as mentioned in this paper.
Journal ArticleDOI
Rapid Single-Step Induction of Functional Neurons from Human Pluripotent Stem Cells
Yingsha Zhang,ChangHui Pak,Yan Han,Henrik Ahlenius,Zhenjie Zhang,Soham Chanda,Samuele Marro,Christopher Patzke,Claudio Acuna,Jason P. Covy,Wei Xu,Nan Yang,Tamas Danko,Lu Chen,Marius Wernig,Thomas C. Südhof +15 more
TL;DR: It is shown that human ESCs and iPSCs can be converted into functional iN cells with nearly 100% yield and purity in less than 2 weeks by forced expression of a single transcription factor.
Journal ArticleDOI
In vivo direct reprogramming of reactive glial cells into functional neurons after brain injury and in an Alzheimer's disease model.
TL;DR: It is shown that reactive glial cells in the cortex of stab-injured or Alzheimer's disease model mice can be directly reprogrammed into functional neurons in vivo using retroviral expression of a single neural transcription factor, NeuroD1.
Journal ArticleDOI
Hierarchical mechanisms for direct reprogramming of fibroblasts to neurons.
Orly L. Wapinski,Thomas Vierbuchen,Kun Qu,Qian Yi Lee,Soham Chanda,Daniel R. Fuentes,Paul G. Giresi,Yi Han Ng,Samuele Marro,Norma F. Neff,Daniela Drechsel,Ben Martynoga,Diogo S. Castro,Ashley E. Webb,Thomas C. Südhof,Anne Brunet,François Guillemot,Howard Y. Chang,Howard Y. Chang,Marius Wernig +19 more
TL;DR: A precise match between pioneer factors and the chromatin context at key target genes is determinative for transdifferentiation to neurons and likely other cell types.
Journal ArticleDOI
Direct reprogramming of mouse and human fibroblasts into multipotent neural stem cells with a single factor.
Karen Ring,Leslie M. Tong,Maureen E. Balestra,Robyn M. Javier,Yaisa Andrews-Zwilling,Gang Li,David Walker,William Zhang,Anatol C. Kreitzer,Yadong Huang +9 more
TL;DR: Self-renewable and multipotent iNSCs without tumorigenic potential can be generated directly from fibroblasts by reprogramming with a single factor, Sox2.
References
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Journal ArticleDOI
Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors
Kazutoshi Takahashi,Koji Tanabe,Mari Ohnuki,Megumi Narita,Tomoko Ichisaka,Kiichiro Tomoda,Shinya Yamanaka +6 more
TL;DR: It is demonstrated that iPS cells can be generated from adult human fibroblasts with the same four factors: Oct3/4, Sox2, Klf4, and c-Myc.
Journal ArticleDOI
Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets
TL;DR: In a four-genome analysis of 3' UTRs, approximately 13,000 regulatory relationships were detected above the estimate of false-positive predictions, thereby implicating as miRNA targets more than 5300 human genes, which represented 30% of the gene set.
Journal ArticleDOI
Microarray analysis shows that some microRNAs downregulate large numbers of target mRNAs
Lee P. Lim,Nelson C. Lau,Philip W. Garrett-engele,Andrew Grimson,Janell M. Schelter,John C. Castle,David P. Bartel,Peter S. Linsley,Jason M. Johnson +8 more
TL;DR: These results suggest that metazoan miRNAs can reduce the levels of many of their target transcripts, not just the amount of protein deriving from these transcripts, and seem to downregulate a far greater number of targets than previously appreciated.
Journal ArticleDOI
Identification of tissue-specific microRNAs from mouse
Mariana Lagos-Quintana,Reinhard Rauhut,Abdullah Yalcin,Jutta Meyer,Winfried Lendeckel,Thomas Tuschl +5 more
TL;DR: 34 novel miRNAs were identified by tissue-specific cloning of approximately 21-nucleotide RNAs from mouse and a miRNA was identified that appears to be the fruitfly and mammalian ortholog of C. elegans lin-4 stRNA.
Journal ArticleDOI
Expression of a single transfected cDNA converts fibroblasts to myoblasts.
TL;DR: In this article, the major open reading frame encoded by this cDNA contains a short protein segment similar to a sequence present in the myc protein family, and the expression of one of these cDNAs transfected into C3H10T1/2 fibroblasts, where it is not normally expressed, is sufficient to convert them to stable myoblasts.
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Massimiliano Caiazzo,Maria Teresa Dell’Anno,Elena Dvoretskova,Dejan Lazarevic,Dejan Lazarevic,Stefano Taverna,Damiana Leo,Tatyana D. Sotnikova,Andrea Menegon,Paola Roncaglia,Giorgia Colciago,Giovanni Russo,Piero Carninci,Gianni Pezzoli,Raul R. Gainetdinov,Stefano Gustincich,Stefano Gustincich,Alexander Dityatev,Vania Broccoli +18 more