Ferrostatin-1 alleviates lipopolysaccharide-induced acute lung injury via inhibiting ferroptosis
Pengfei Liu,Pengfei Liu,Yetong Feng,Hanwei Li,Hanwei Li,Xin Chen,Guangsuo Wang,Shiyuan Xu,Yalan Li,Lei Zhao,Lei Zhao +10 more
TLDR
It is indicated that ferroptosis has an important role in the progression of LPS-induced ALI, and ferroPTosis may become a novel target in the treatment of ALI patients.Abstract:
Ferroptosis is a newly recognized type of cell death, which is different from traditional necrosis, apoptosis or autophagic cell death. However, the position of ferroptosis in lipopolysaccharide (LPS)-induced acute lung injury (ALI) has not been explored intensively so far. In this study, we mainly analyzed the relationship between ferroptosis and LPS-induced ALI. In this study, a human bronchial epithelial cell line, BEAS-2B, was treated with LPS and ferrostatin-1 (Fer-1, ferroptosis inhibitor). The cell viability was measured using CCK-8. Additionally, the levels of malondialdehyde (MDA), 4-hydroxynonenal (4-HNE), and iron, as well as the protein level of SLC7A11 and GPX4, were measured in different groups. To further confirm the in vitro results, an ALI model was induced by LPS in mice, and the therapeutic action of Fer-1 and ferroptosis level in lung tissues were evaluated. The cell viability of BEAS-2B was down-regulated by LPS treatment, together with the ferroptosis markers SLC7A11 and GPX4, while the levels of MDA, 4-HNE and total iron were increased by LPS treatment in a dose-dependent manner, which could be rescued by Fer-1. The results of the in vivo experiment also indicated that Fer-1 exerted therapeutic action against LPS-induced ALI, and down-regulated the ferroptosis level in lung tissues. Our study indicated that ferroptosis has an important role in the progression of LPS-induced ALI, and ferroptosis may become a novel target in the treatment of ALI patients.read more
Citations
More filters
Journal ArticleDOI
Cycloacceleration of ferroptosis and calcicoptosis for magnetic resonance imaging-guided colorectal cancer therapy
Shuai Guo,Zong Li,Jie Feng,Wei-Dong Xiong,Jingzhen Yang,Xuanyi Lu,Sugeun Yang,Yikai Xu,Aiguo Wu,Zheyu Shen +9 more
TL;DR: In this article , the authors proposed a new concept of cycloacceleration of ferroptosis and calcicoptosis for the magnetic resonance imaging (MRI)-guided colorectal cancer (CRC) therapy.
Journal ArticleDOI
Mitoquinone alleviates bleomycin-induced acute lung injury via inhibiting mitochondrial ROS-dependent pulmonary epithelial ferroptosis.
Ping Zhan,Xue Lu,Zhao Li,Wei Wang,Kun D. Peng,Nan-Nan Liang,Yan Wang,Jian Li,Lin Fu,Hui Zhao,De-Xiang Xu,Zhu-Xia Tan +11 more
TL;DR: In this paper , the role of mitochondrial reactive oxygen species (ROS) on acute lung injury (ALI) and pulmonary epithelial ferroptosis was explored, and mitochondria-targeted antioxidants may be used as potential therapeutic agents for BLM-induced ALI.
Journal ArticleDOI
The Role Played by Ferroptosis in Osteoarthritis: Evidence Based on Iron Dyshomeostasis and Lipid Peroxidation
TL;DR: Recent evidence is summarized by focusing on ferroptotic mechanisms and the role played by ferroPTosis in OA pathogenesis from the perspectives of clinical findings, animal models, and cell research.
Journal ArticleDOI
Potential Role of APEX1 During Ferroptosis
TL;DR: The possibility to employ APEX1 inducers or inhibitors in the regulation of ferroptosis as a new strategy for the treatment of various human diseases is discussed.
Journal ArticleDOI
PRDX6 alleviates lipopolysaccharide-induced inflammation and ferroptosis in periodontitis
Wenjun Yang,Xiang Meng,Yue-rong Wang,Qing-qing Wang,Xin He,Xiao Yu Sun,NanNan Cheng,Li Zhang +7 more
TL;DR: In this paper , the expression of peroxiredoxin 6 (PRDX6) and nuclear factor erythropoietin 2-related factor 2 (NRF2) was investigated to protect human gingival fibroblasts (HGFs) from lipopolysaccharide (LPS)-induced inflammation and its mechanisms.
References
More filters
Journal ArticleDOI
Ferroptosis: A Regulated Cell Death Nexus Linking Metabolism, Redox Biology, and Disease
Brent R. Stockwell,José Pedro Friedmann Angeli,Hülya Bayır,Ashley I. Bush,Marcus Conrad,Scott J. Dixon,Simone Fulda,Susan Gascon,Stavroula K. Hatzios,Valerian E. Kagan,Kay Noel,Xuejun Jiang,Andreas Linkermann,Maureen E. Murphy,Michael Overholtzer,Atsushi Oyagi,Gabriela Carolina Pagnussat,Jason Park,Qitao Ran,Craig S. Rosenfeld,Konstantin Salnikow,Daolin Tang,Daolin Tang,Frank M. Torti,Suzy V. Torti,Shinya Toyokuni,K. A. Woerpel,Donna D. Zhang +27 more
TL;DR: The mechanisms underlying ferroptosis are reviewed, connections to other areas of biology and medicine are highlighted, and tools and guidelines for studying this emerging form of regulated cell death are recommended.
Journal ArticleDOI
Inactivation of the ferroptosis regulator Gpx4 triggers acute renal failure in mice
José Pedro Friedmann Angeli,Manuela Schneider,Bettina Proneth,Yulia Y. Tyurina,Vladimir A. Tyurin,Victoria Jayne Hammond,Nadja Herbach,Michaela Aichler,Axel Walch,E. Eggenhofer,Devaraj Basavarajappa,Olof Rådmark,Sho Kobayashi,Tobias Seibt,Heike Beck,Frauke Neff,Irene Esposito,Rüdiger Wanke,Heidi Förster,Olena Yefremova,Marc Heinrichmeyer,Georg W. Bornkamm,Edward K. Geissler,Stephen B. Thomas,Brent R. Stockwell,Valerie B. O'Donnell,Valerian E. Kagan,Joel A. Schick,Marcus Conrad +28 more
TL;DR: It is demonstrated that ferroptosis is a pervasive and dynamic form of cell death, which, when impeded, promises substantial cytoprotection.
Journal ArticleDOI
Ferroptosis: process and function.
Yangchun Xie,Wen Hou,Xinxin Song,Yan Yu,Jin Huang,Xiaofang Sun,Rui Kang,Daolin Tang,Daolin Tang +8 more
TL;DR: Misregulated ferroptosis has been implicated in multiple physiological and pathological processes, including cancer cell death, neurotoxicity, neurodegenerative diseases, acute renal failure, drug-induced hepatotoxicity, hepatic and heart ischemia/reperfusion injury, and T-cell immunity.
Journal ArticleDOI
Animal models of acute lung injury.
TL;DR: The goal of this review is to summarize the strengths and weaknesses of existing models of lung injury and help guide investigators in the design and interpretation of animal studies of acute lung injury.
Journal ArticleDOI
Mechanisms of ferroptosis
TL;DR: Outstanding questions include the relationship between ferroPTosis and other forms of cell death, and whether activation or inhibition of ferroptosis can be exploited to achieve desirable therapeutic ends.
Related Papers (5)
Regulation of Ferroptotic Cancer Cell Death by GPX4
Inactivation of the ferroptosis regulator Gpx4 triggers acute renal failure in mice
José Pedro Friedmann Angeli,Manuela Schneider,Bettina Proneth,Yulia Y. Tyurina,Vladimir A. Tyurin,Victoria Jayne Hammond,Nadja Herbach,Michaela Aichler,Axel Walch,E. Eggenhofer,Devaraj Basavarajappa,Olof Rådmark,Sho Kobayashi,Tobias Seibt,Heike Beck,Frauke Neff,Irene Esposito,Rüdiger Wanke,Heidi Förster,Olena Yefremova,Marc Heinrichmeyer,Georg W. Bornkamm,Edward K. Geissler,Stephen B. Thomas,Brent R. Stockwell,Valerie B. O'Donnell,Valerian E. Kagan,Joel A. Schick,Marcus Conrad +28 more
Ferroptosis: A Regulated Cell Death Nexus Linking Metabolism, Redox Biology, and Disease
Brent R. Stockwell,José Pedro Friedmann Angeli,Hülya Bayır,Ashley I. Bush,Marcus Conrad,Scott J. Dixon,Simone Fulda,Susan Gascon,Stavroula K. Hatzios,Valerian E. Kagan,Kay Noel,Xuejun Jiang,Andreas Linkermann,Maureen E. Murphy,Michael Overholtzer,Atsushi Oyagi,Gabriela Carolina Pagnussat,Jason Park,Qitao Ran,Craig S. Rosenfeld,Konstantin Salnikow,Daolin Tang,Daolin Tang,Frank M. Torti,Suzy V. Torti,Shinya Toyokuni,K. A. Woerpel,Donna D. Zhang +27 more
ACSL4 dictates ferroptosis sensitivity by shaping cellular lipid composition
Sebastian Doll,Bettina Proneth,Yulia Y. Tyurina,Elena Panzilius,Sho Kobayashi,Irina Ingold,Martin Irmler,Johannes Beckers,Michaela Aichler,Axel Walch,Holger Prokisch,Dietrich Trümbach,Gaowei Mao,Feng Qu,Hülya Bayır,Joachim Füllekrug,Christina Scheel,Wolfgang Wurst,Joel A. Schick,Valerian E. Kagan,José Pedro Friedmann Angeli,Marcus Conrad +21 more