Ferrostatin-1 alleviates lipopolysaccharide-induced acute lung injury via inhibiting ferroptosis
Pengfei Liu,Pengfei Liu,Yetong Feng,Hanwei Li,Hanwei Li,Xin Chen,Guangsuo Wang,Shiyuan Xu,Yalan Li,Lei Zhao,Lei Zhao +10 more
TLDR
It is indicated that ferroptosis has an important role in the progression of LPS-induced ALI, and ferroPTosis may become a novel target in the treatment of ALI patients.Abstract:
Ferroptosis is a newly recognized type of cell death, which is different from traditional necrosis, apoptosis or autophagic cell death. However, the position of ferroptosis in lipopolysaccharide (LPS)-induced acute lung injury (ALI) has not been explored intensively so far. In this study, we mainly analyzed the relationship between ferroptosis and LPS-induced ALI. In this study, a human bronchial epithelial cell line, BEAS-2B, was treated with LPS and ferrostatin-1 (Fer-1, ferroptosis inhibitor). The cell viability was measured using CCK-8. Additionally, the levels of malondialdehyde (MDA), 4-hydroxynonenal (4-HNE), and iron, as well as the protein level of SLC7A11 and GPX4, were measured in different groups. To further confirm the in vitro results, an ALI model was induced by LPS in mice, and the therapeutic action of Fer-1 and ferroptosis level in lung tissues were evaluated. The cell viability of BEAS-2B was down-regulated by LPS treatment, together with the ferroptosis markers SLC7A11 and GPX4, while the levels of MDA, 4-HNE and total iron were increased by LPS treatment in a dose-dependent manner, which could be rescued by Fer-1. The results of the in vivo experiment also indicated that Fer-1 exerted therapeutic action against LPS-induced ALI, and down-regulated the ferroptosis level in lung tissues. Our study indicated that ferroptosis has an important role in the progression of LPS-induced ALI, and ferroptosis may become a novel target in the treatment of ALI patients.read more
Citations
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Journal ArticleDOI
Ferritinophagy-mediated ferroptosis is involved in sepsis-induced cardiac injury
Ning Li,Wei Wang,Heng Zhou,Qing-Qing Wu,Mingxia Duan,Chen Liu,Hai-Ming Wu,Wei Deng,Difei Shen,Qi-Zhu Tang +9 more
TL;DR: It is concluded that ferritinophagy-mediated ferroptosis is one of the critical mechanisms contributing to sepsis-induced cardiac injury and Targeting ferroPTosis in cardiomyocytes may be a therapeutic strategy for preventing sepsi in the future.
Journal ArticleDOI
Ferritinophagy and ferroptosis in the management of metabolic diseases.
Amir Ajoolabady,Hamid Aslkhodapasandhokmabad,Peter Libby,Jaakko Tuomilehto,Jaakko Tuomilehto,Jaakko Tuomilehto,Gregory Y.H. Lip,Josef M. Penninger,Josef M. Penninger,Des R. Richardson,Des R. Richardson,Des R. Richardson,Daolin Tang,Hao Zhou,Hao Zhou,Shuyi Wang,Shuyi Wang,Daniel J. Klionsky,Guido Kroemer,Jun Ren,Jun Ren +20 more
TL;DR: In this article, the authors delineate the role of ferritinophagy in ferroptosis, and its underlying regulatory mechanisms, to unveil the therapeutic value of the selective form of autophagy as a target in the combat of metabolic diseases.
Journal ArticleDOI
Panaxydol attenuates ferroptosis against LPS-induced acute lung injury in mice by Keap1-Nrf2/HO-1 pathway.
Jiucui Li,Kongmiao Lu,Fenglan Sun,Shanjuan Tan,Xiao Zhang,Wei Sheng,Wanming Hao,Min Liu,Weihong Lv,Wei Han +9 more
TL;DR: Panaxydol (PX) isolated from the roots of Panax ginseng, has been shown to attenuate ferroptosis against LPS-induced acute lung injury (ALI)/acute respiratory distress syndrome (ARDS) in mice.
Journal ArticleDOI
Nrf2 protects against seawater drowning-induced acute lung injury via inhibiting ferroptosis.
Yu-bao Qiu,Bin-bin Wan,Gang Liu,Ya-Xian Wu,Dan Chen,Mu-dan Lu,Jun-Liang Chen,Renqiang Yu,Daozhen Chen,Qing-feng Pang +9 more
TL;DR: Results suggested that Nrf2 can inhibit ferroptosis and therefore alleviate ALI induced by seawater drowning, providing a novel therapeutic target for seaw water drowning-induced ALI.
Journal ArticleDOI
Nanoparticle-induced ferroptosis: detection methods, mechanisms and applications.
Huizhen Zheng,Jun Jiang,Shujuan Xu,Wei Liu,Qianqian Xie,Xiaoming Cai,Jie Zhang,Sijin Liu,Ruibin Li +8 more
TL;DR: In this article, the authors discuss the molecular initiating events of nanosized ferroptosis inducers and the cascade signals in cells, and elaborate the cell death mechanism, and the key physicochemical properties of nano-inducers are also discussed to acquire a fundamental understanding of nan-structure-activity relationships (nano-SARs) involved in cell death.
References
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Journal ArticleDOI
Inhibition of neuronal ferroptosis in the acute phase of intracerebral hemorrhage shows long-term cerebroprotective effects.
Bin Chen,Zhenghong Chen,Mingjian Liu,Xiaorong Gao,Yijun Cheng,Yongxu Wei,Zhe Bao Wu,Derong Cui,Hanbing Shang +8 more
TL;DR: It is demonstrated that neuronal ferroPTosis occurs during the acute phase of ICH in brain areas distant from the hematoma and that inhibition of ferroptosis by Fer-1 exerted a long-term cerebroprotective effect.
Journal ArticleDOI
The origin and future of oxidative stress pathology: From the recognition of carcinogenesis as an iron addiction with ferroptosis‐resistance to non‐thermal plasma therapy
Shinya Toyokuni,Shinya Toyokuni +1 more
TL;DR: Antigens were sought for this purpose based on an oxidative stress‐induced rat renal carcinogenesis model, which revealed that 8‐hydroxy‐2′‐deoxyguanosine and 4‐hydrox‐2‐nonenal‐modified proteins are ideal and Counteracting excess iron is a promising preventive strategy for major diseases.
Journal ArticleDOI
Astrocyte hepcidin is a key factor in LPS-induced neuronal apoptosis
Linhao You,Cai-Zhen Yan,Cai-Zhen Yan,Bing-Jie Zheng,Yun-Zhe Ci,Shiyang Chang,Peng Yu,Guofen Gao,Haiyan Li,Tian-Yu Dong,Yan-Zhong Chang +10 more
TL;DR: It is demonstrated that the expression of the systemic iron regulatory hormone, hepcidin, is induced by lipopolysaccharide (LPS) through the IL-6/STAT3 pathway in the cortex and hippocampus, consistent with a model whereby inflammation initiates an intercellular signaling cascade in which activated microglia stimulate astrocytes to release hePCidin which, in turn, signals to neurons to prevent their iron release.
Journal ArticleDOI
Ulinastatin Protects Against LPS-Induced Acute Lung Injury By Attenuating TLR4/NF-κB Pathway Activation and Reducing Inflammatory Mediators.
TL;DR: Findings indicate that UTI ameliorates LPS-induced ALI by attenuating the TLR4/NF-&kgr;B pathway activation.
Journal ArticleDOI
Role of ferroptosis in the process of acute radiation-induced lung injury in mice
TL;DR: It is observed that ferroPTosis played a crucial role in acute RILI, and the ROS induced by irradaition might be the original trigger of ferroptosis in acuteRILI.
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