Forward and Reverse Genetics through Derivation of Haploid Mouse Embryonic Stem Cells
Ulrich Elling,Jasmin Taubenschmid,Gerald Wirnsberger,Ronan C. O'Malley,Simon-Pierre Demers,Quentin Vanhaelen,Andrey I. Shukalyuk,Gerald Schmauss,Daniel Schramek,Frank Schnuetgen,Harald von Melchner,Joseph R. Ecker,Joseph R. Ecker,William L. Stanford,William L. Stanford,William L. Stanford,Johannes Zuber,Alexander Stark,Josef M. Penninger +18 more
TLDR
The generation of haploid mouse ESC lines from parthenogenetic embryos is reported, opening the possibility of combining the power of a haploid genome with pluripotency of embryonic stem cells to uncover fundamental biological processes in defined cell types at a genomic scale.About:
This article is published in Cell Stem Cell.The article was published on 2011-12-02 and is currently open access. It has received 214 citations till now. The article focuses on the topics: Cellular differentiation & Somatic cell.read more
Citations
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Genome-wide recessive genetic screening in mammalian cells with a lentiviral CRISPR-guide RNA library
TL;DR: The results demonstrate the potential for efficient loss-of-function screening using the CRISPR-Cas9 system and identify 27 known and 4 previously unknown genes implicated in these phenotypes.
Journal ArticleDOI
Target Identification for Small Bioactive Molecules: Finding the Needle in the Haystack
TL;DR: Current methods for target identification of small molecules are summarized, primarily for a chemistry audience but also the biological community, for example, the chemist or biologist attempting to identify the target of a given bioactive compound.
Journal ArticleDOI
Thiol-linked alkylation of RNA to assess expression dynamics.
Veronika A. Herzog,Brian Reichholf,Tobias Neumann,Philipp Rescheneder,Pooja Bhat,Thomas R Burkard,Wiebke Wlotzka,Arndt von Haeseler,Johannes Zuber,Stefan L. Ameres +9 more
TL;DR: Thiol(SH)-linked alkylation for the metabolic sequencing of RNA (SLAM seq), an orthogonal-chemistry-based RNA sequencing technology that detects 4-thiouridine incorporation in RNA species at single-nucleotide resolution, facilitates the dissection of fundamental mechanisms that control gene expression in an accessible, cost-effective and scalable manner.
Journal ArticleDOI
The ‘obligate diploid’ Candida albicans forms mating-competent haploids
Meleah A. Hickman,Guisheng Zeng,Anja Forche,Matthew P. Hirakawa,Darren Abbey,Benjamin D. Harrison,Yan-Ming Wang,Ching Hua Su,Richard J. Bennett,Yue Wang,Judith Berman,Judith Berman +11 more
TL;DR: It is demonstrated that C. albicans has a viable haploid state that can be derived from diploid cells under in vitro and in vivo conditions, and that seems to arise through a concerted chromosome loss mechanism.
Journal ArticleDOI
Megabase-scale deletion using CRISPR/Cas9 to generate a fully haploid human cell line
Patrick Essletzbichler,Tomasz Konopka,Federica Santoro,Doris Chen,Bianca V. Gapp,Robert Kralovics,Thijn R. Brummelkamp,Thijn R. Brummelkamp,Sebastian M.B. Nijman,Tilmann Bürckstümmer +9 more
TL;DR: In this paper, the authors employed a CRISPR/Cas9-based genome engineering strategy to excise this sizeable chromosomal fragment and to efficiently and reproducibly derive clones that retain their haploid state.
References
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TL;DR: Induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions is demonstrated and iPS cells, designated iPS, exhibit the morphology and growth properties of ES cells and express ES cell marker genes.
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The nuclear RNase III Drosha initiates microRNA processing
Yoontae Lee,Chiyoung Ahn,Jinju Han,Hyounjeong Choi,Jaekwang Kim,Jeongbin Yim,Junho Lee,Patrick Provost,Olof Rådmark,Sun-Young Kim,V. Narry Kim +10 more
TL;DR: The two RNase III proteins, Drosha and Dicer, may collaborate in the stepwise processing of miRNAs, and have key roles in miRNA-mediated gene regulation in processes such as development and differentiation.
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A System for Stable Expression of Short Interfering RNAs in Mammalian Cells
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Mutations affecting segment number and polarity in Drosophila
TL;DR: The phenotypes of the mutant embryos indicate that the process of segmentation involves at least three levels of spatial organization: the entire egg as developmental unit, a repeat unit with the length of two segments, and the individual segment.
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Functional expression cloning of nanog, a pluripotency sustaining factor in embryonic stem cells
Ian Chambers,Douglas Colby,Morag Robertson,Jennifer Nichols,Sonia Lee,Susan Tweedie,Austin Smith +6 more
TL;DR: These findings establish a central role for Nanog in the transcription factor hierarchy that defines ES cell identity and confirm that Cytokine dependence, multilineage differentiation, and embryo colonization capacity are fully restored upon transgene excision.
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