Gene expression patterns in human embryonic stem cells and human pluripotent germ cell tumors
Jamie M. Sperger,Xin Chen,Jonathan S. Draper,Jessica E. Antosiewicz,Chris H. Chon,Sunita B. Jones,James D. Brooks,Peter W. Andrews,Patrick O. Brown,James A. Thomson +9 more
TLDR
The gene expression patterns of human ES cell lines showed many similarities with the human embryonal carcinoma cell samples and more distantly with the seminoma samples, and 895 genes were identified that are candidates for involvement in the maintenance of a pluripotent, undifferentiated phenotype.Abstract:
Remarkably little is known about the transcriptional profiles of human embryonic stem (ES) cells or the molecular mechanisms that underlie their pluripotency. To identify commonalties among the transcriptional profiles of different human pluripotent cells and to search for clues into the genesis of human germ cell tumors, we compared the expression profiles of human ES cell lines, human germ cell tumor cell lines and tumor samples, somatic cell lines, and testicular tissue samples by using cDNA microarray analysis. Hierarchical cluster analysis of gene expression profiles showed that the five independent human ES cell lines clustered tightly together, reflecting highly similar expression profiles. The gene expression patterns of human ES cell lines showed many similarities with the human embryonal carcinoma cell samples and more distantly with the seminoma samples. We identified 895 genes that were expressed at significantly greater levels in human ES and embryonal carcinoma cell lines than in control samples. These genes are candidates for involvement in the maintenance of a pluripotent, undifferentiated phenotype.read more
Citations
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Journal ArticleDOI
An embryonic stem cell–like gene expression signature in poorly differentiated aggressive human tumors
Ittai Ben-Porath,Matt Thomson,Vincent J. Carey,Ruping Ge,George W. Bell,Aviv Regev,Robert A. Weinberg +6 more
TL;DR: The results reveal a previously unknown link between genes associated with ES cell identity and the histopathological traits of tumors and support the possibility that these genes contribute to stem cell–like phenotypes shown by many tumors.
Journal ArticleDOI
Derivation of human embryonic stem cells in defined conditions.
Tenneille Ludwig,Mark E. Levenstein,Jeffrey M. Jones,Jeffrey M. Jones,W. Travis Berggren,Erika R Mitchen,Jennifer L. Frane,Leann J Crandall,Christine A. Daigh,Kevin R. Conard,Marian S Piekarczyk,Rachel A. Llanas,James A. Thomson +12 more
TL;DR: Feeder-independent human ES cell culture that includes protein components solely derived from recombinant sources or purified from human material is reported.
Journal ArticleDOI
Embryonic stem cells.
Helen J. Rippon,Anne E. Bishop +1 more
TL;DR: This work reviews the history of murine and human ES cell Lines, including practical and ethical aspects of ES cell isolation from pre‐implantation embryos, maintenance of undifferentiated ES cell lines in the cell culture environment, and differentiation of ES cells in vitro and in vivo into mature somatic cell types.
Journal ArticleDOI
Basic FGF and suppression of BMP signaling sustain undifferentiated proliferation of human ES cells
Ren-He Xu,Ren-He Xu,Ruthann M. Peck,Dong S. Li,Xuezhu Feng,Tenneille Ludwig,James A. Thomson,James A. Thomson +7 more
TL;DR: It is demonstrated that hESCs cultured in unconditioned medium (UM) are subjected to high levels of B MP signaling activity, which is reduced in CM, and the BMP antagonist noggin synergizes with basic fibroblast growth factor (bFGF) to repress BMP signaling and sustain undifferentiated proliferation of hESC in the absence of fibroblasts or CM.
Journal ArticleDOI
Characterization of human embryonic stem cell lines by the International Stem Cell Initiative
Oluseun Adewumi,Behrouz Aflatoonian,Lars Ährlund-Richter,Michal Amit,Peter W. Andrews,Gemma Beighton,Paul Bello,Nissim Benvenisty,Lorraine S. Berry,Simon Bevan,Barak Blum,Justin Brooking,Kevin G. Chen,Andre Bh Choo,Gary A. Churchill,Marie Corbel,Ivan Damjanov,John S Draper,Petr Dvorak,Petr Dvorak,Katarina Emanuelsson,Roland A. Fleck,Angela Ford,Karin Astrid Maria Gertow,Karin Astrid Maria Gertow,Marina Gertsenstein,Paul J. Gokhale,Rebecca S. Hamilton,Alex Hampl,Alex Hampl,Lyn Healy,Outi Hovatta,Johan Hyllner,Marta P. Imreh,Marta P. Imreh,Joseph Itskovitz-Eldor,Jamie P. Jackson,Jackie Johnson,Mark Jones,Kehkooi Kee,Benjamin L. King,Barbara B. Knowles,Majlinda Lako,Franck Lebrin,Barbara S. Mallon,Daisy Manning,Yoav Mayshar,Ronald D.G. McKay,Anna E. Michalska,Milla Mikkola,Masha Mileikovsky,Stephen L. Minger,Harry Moore,Christine L. Mummery,Andras Nagy,Norio Nakatsuji,Carmel M. O’Brien,Steve Oh,Cia Olsson,Timo Otonkoski,Kye-Yoon Park,Robert Passier,Hema Patel,Minal Patel,Roger A. Pedersen,Martin F. Pera,Marian S Piekarczyk,Renee A. Reijo Pera,Benjamin Reubinoff,Allan J. Robins,Janet Rossant,Peter J. Rugg-Gunn,Peter J. Rugg-Gunn,Thomas C Schulz,Henrik Semb,Eric S Sherrer,Henrike Siemen,Glyn Stacey,Miodrag Stojkovic,Hirofumi Suemori,Jin P. Szatkiewicz,Tikva Turetsky,Timo Tuuri,Steineke van den Brink,Kristina Vintersten,Sanna Vuoristo,Dorien Ward,Thomas A Weaver,Lesley Young,Weidong Zhang +89 more
TL;DR: The International Stem Cell Initiative characterized 59 human embryonic stem cell lines from 17 laboratories worldwide and found that despite diverse genotypes and different techniques used for derivation and maintenance, all lines exhibited similar expression patterns for several markers ofhuman embryonic stem cells.
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TL;DR: It is shown that there is diversity in gene expression among the tumours of DLBCL patients, apparently reflecting the variation in tumour proliferation rate, host response and differentiation state of the tumour.
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