Gsh-1: a novel murine homeobox gene expressed in the central nervous system
M. Todd Valerius,Hung Li,Jeffrey L. Stock,Michael Weinstein,Satbir Kaur,Gurparkash Singh,S. Steven Potter +6 more
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TLDR
The characterization of Gsh‐1, a novel murine homeobox gene, is reported, with a transcript of approximately 2 kb in size present at embryonic days 10.5 to E13.5 and the consensus DNA ninding site of the GSh‐1 homeoprotein is determined to be GCT/CA/CATTAG/A.Abstract:
We report the characterization of Gsh-1, a novel murine homeobox gene. Northern blot analysis revealed a transcript of approximately 2 kb in size present at embryonic days 10.5, 11.5, and 12.5 of development. The cDNA sequence encoded a proline rich motif, a polyalanine tract, and a homeodomain with strong homology to those encoded by the clustered Hox genes. The Gsh-1 expression pattern was determined for days E8.5 to E13.5 by whole mount and serial section in situ hybridizations. Gsh-1 transcription was restricted to the central nervous system. Expression is present in the neural tube and hindbrain as two continuous, bilaterally symmetrical stripes within neural epithelial tissue. In the mesencephalon, expression is seen as a band across the most anterior portion. There is also diencephalon expression in the anlagen of the thalamus and the hypothalamus as well as in the optic stalk, optic recess, and the ganglionic eminence. Moreover, through the use of fusion proteins containing the Gsh-1 homeodomain, we have determined the consensus DNA ninding site of the Gsh-1 homeoprotein to be GCT/CA/CATTAG/A. ©1995 Wiley-Liss, Inc.read more
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Book ChapterDOI
Mechanisms and Evolution of Dorsal–Ventral Patterning
TL;DR: The progression of the field from early observations made more than a century ago is discussed and future challenges regarding the problem of scaling of the nervous system during evolution are introduced.
Book ChapterDOI
Transcriptional Control of the Development and Function of the Hypothalamic-Pituitary Axis
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Dissertation
Anàlisi de l'expressió dels gens ParaHox al páncrees endocrí
TL;DR: In this article, a majoria destudis fets sobre lexpressio dels gens ParaHox shavien realitzat en embrions, es va decidir analitzar lexpression dels Gens ParAHox en diversos teixits adults de ratoli.
The ParaHox gene Gsx patterns the apical organ and central nervous system but not the foregut in scaphopod and cephalopod mollusks
Tim Wollesen,Sonia Victoria Rodríguez Monje,Carmel McDougall,Bernard M. Degnan,Andreas Wanninger +4 more
TL;DR: It is suggested that Gsx expression in the foregut might not be a plesiomorphic trait of the Lophotrochozoa as insinuated previously and a role in gut formation in the last common bilaterian ancestor appears unlikely.
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Journal ArticleDOI
Homeobox genes and axial patterning
William McGinnis,Robb Krumlauf +1 more
TL;DR: Over the past seven years, the term “homeodomain” has evolved to define a class of protein domains that have recognizable similarity to a 60 amino acid motif originally recognized in three Drosophila homeotic and segmentation proteins.