Gsh-1: a novel murine homeobox gene expressed in the central nervous system
M. Todd Valerius,Hung Li,Jeffrey L. Stock,Michael Weinstein,Satbir Kaur,Gurparkash Singh,S. Steven Potter +6 more
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TLDR
The characterization of Gsh‐1, a novel murine homeobox gene, is reported, with a transcript of approximately 2 kb in size present at embryonic days 10.5 to E13.5 and the consensus DNA ninding site of the GSh‐1 homeoprotein is determined to be GCT/CA/CATTAG/A.Abstract:
We report the characterization of Gsh-1, a novel murine homeobox gene. Northern blot analysis revealed a transcript of approximately 2 kb in size present at embryonic days 10.5, 11.5, and 12.5 of development. The cDNA sequence encoded a proline rich motif, a polyalanine tract, and a homeodomain with strong homology to those encoded by the clustered Hox genes. The Gsh-1 expression pattern was determined for days E8.5 to E13.5 by whole mount and serial section in situ hybridizations. Gsh-1 transcription was restricted to the central nervous system. Expression is present in the neural tube and hindbrain as two continuous, bilaterally symmetrical stripes within neural epithelial tissue. In the mesencephalon, expression is seen as a band across the most anterior portion. There is also diencephalon expression in the anlagen of the thalamus and the hypothalamus as well as in the optic stalk, optic recess, and the ganglionic eminence. Moreover, through the use of fusion proteins containing the Gsh-1 homeodomain, we have determined the consensus DNA ninding site of the Gsh-1 homeoprotein to be GCT/CA/CATTAG/A. ©1995 Wiley-Liss, Inc.read more
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Gsh2 and Pax6 play complementary roles in dorsoventral patterning of the mammalian telencephalon
TL;DR: Evidence is presented that the progenitor and postmitotic cells flanking the pallial/subpallial boundary (PSB) in the embryonic mouse can be subdivided into multiple regions that express unique combinations of transcription factors.
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Genetic control of dorsal-ventral identity in the telencephalon: opposing roles for Pax6 and Gsh2.
TL;DR: Results demonstrate that Pax6 and Gsh2 govern cortical and striatal development by regulating genetically opposing programs that control the expression of each other as well as the regionally expressed developmental regulators Mash1, the neurogenins and Dlx genes in telencephalic progenitors.
Journal ArticleDOI
Multiple Dorsoventral Origins of Oligodendrocyte Generation in the Spinal Cord and Hindbrain
TL;DR: It is proposed that oligodendrocytes derive from several distinct positional origins and that the activation of Olig1/2 at different positions is controlled by distinct genetic programs.
Journal ArticleDOI
Ventral neural patterning by Nkx homeobox genes: Nkx6.1 controls somatic motor neuron and ventral interneuron fates
Maike Sander,Sussan Paydar,Johan Ericson,James Briscoe,Elisabeth Berber,Michael S. German,Thomas M. Jessell,John L.R. Rubenstein +7 more
TL;DR: The patterns of neurogenesis in mice carrying a targeted mutation in Nkx6.1 show a dorsal-to-ventral switch in the identity of progenitors and in the fate of postmitotic neurons, demonstrating the essential functions of NkX6.
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Pax-6 functions in boundary formation and axon guidance in the embryonic mouse forebrain.
TL;DR: It is concluded that Pax-6 plays multiple roles in forebrain patterning, including boundary formation, regionally restricted patterns in the developing brain and eye, neuron specification and axon guidance.
References
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Isolation and characterization of rat and human glyceraldehyde-3-phosphate dehydrogenase cDNAs: genomic complexity and molecular evolution of the gene
TL;DR: Comparison of these two cDNA sequences with those of the chicken, Drosophila and yeast genes allows the analysis of the evolution of the GAPDH genes in detail.
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Msx1 deficient mice exhibit cleft palate and abnormalities of craniofacial and tooth development
Ichiro Satokata,Richard L. Maas +1 more
TL;DR: The Msx1 homeobox gene has a critical role in mediating epithelial–mesenchymal interactions during craniofacial bone and tooth development, and provides a genetic model for cleft palate and oligodontia in which the defective gene is known.
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Homeotic transformations of murine vertebrae and concomitant alteration of Hox codes induced by retinoic acid
Michael Kessel,Peter Gruss +1 more
TL;DR: It is suggested that the identity of a vertebral segment is specified by a combination of functionally active Hox genes, a "Hox code," and that exogenous RA interferes with the normal establishment of Hox codes and thus with axial specification.
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The murine and Drosophila homeobox gene complexes have common features of organization and expression
TL;DR: Alignment shows a clear relationship among genes in the mouse and Drosophila complexes, based on relative position, sequence identity, and domains of expression along the rostral-caudal axis.
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Differences and similarities in DNA-binding preferences of MyoD and E2A protein complexes revealed by binding site selection
TK Blackwell,Harold Weintraub +1 more
TL;DR: It was shown that homo- and heterooligomers of the helix-loop-helix proteins MyoD and E2A recognize a common consensus sequence, CA--TG, but otherwise bind to flanking and internal positions with different sequence preferences that suggest half-site recognition, suggesting that different combinations of dimeric proteins can have different binding sequence preferences.