Gsh-1: a novel murine homeobox gene expressed in the central nervous system
M. Todd Valerius,Hung Li,Jeffrey L. Stock,Michael Weinstein,Satbir Kaur,Gurparkash Singh,S. Steven Potter +6 more
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TLDR
The characterization of Gsh‐1, a novel murine homeobox gene, is reported, with a transcript of approximately 2 kb in size present at embryonic days 10.5 to E13.5 and the consensus DNA ninding site of the GSh‐1 homeoprotein is determined to be GCT/CA/CATTAG/A.Abstract:
We report the characterization of Gsh-1, a novel murine homeobox gene. Northern blot analysis revealed a transcript of approximately 2 kb in size present at embryonic days 10.5, 11.5, and 12.5 of development. The cDNA sequence encoded a proline rich motif, a polyalanine tract, and a homeodomain with strong homology to those encoded by the clustered Hox genes. The Gsh-1 expression pattern was determined for days E8.5 to E13.5 by whole mount and serial section in situ hybridizations. Gsh-1 transcription was restricted to the central nervous system. Expression is present in the neural tube and hindbrain as two continuous, bilaterally symmetrical stripes within neural epithelial tissue. In the mesencephalon, expression is seen as a band across the most anterior portion. There is also diencephalon expression in the anlagen of the thalamus and the hypothalamus as well as in the optic stalk, optic recess, and the ganglionic eminence. Moreover, through the use of fusion proteins containing the Gsh-1 homeodomain, we have determined the consensus DNA ninding site of the Gsh-1 homeoprotein to be GCT/CA/CATTAG/A. ©1995 Wiley-Liss, Inc.read more
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Zebrafish and fly Nkx6 proteins have similar CNS expression patterns and regulate motoneuron formation
Sarah E. Cheesman,Michael J. Layden,Michael J. Layden,Tonia Von Ohlen,Tonia Von Ohlen,Chris Q. Doe,Chris Q. Doe,Judith S. Eisen +7 more
TL;DR: Overexpression of fish or fly Nkx6 is sufficient to generate supernumerary motoneurons in both zebrafish and flies, suggesting that one ancestral function of NKx6 proteins was to promote motoneuron development.
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Morphogenesis of the optic tectum in the medaka (Oryzias latipes): a morphological and molecular study, with special emphasis on cell proliferation.
Vân Nguyen,Karine Deschet,Torsten Henrich,Estelle Godet,Jean-Stéphane Joly,Jochen Wittbrodt,Daniel Chourrout,Franck Bourrat +7 more
TL;DR: The medaka optic tectum morphogenesis is analyzed by using 5‐bromo‐2'‐deoxyuridine (BrdU) immunohistochemistry and in situ hybridization with three probes, two for recently cloned homeobox genes (Ol‐Prx3 [Paired‐Related‐Homeobox3] and Ol‐Gsh1 [Genetic‐Screen‐ Homeobox1]) and one for Ol‐tailless.
Journal ArticleDOI
Control of neurogenesis--lessons from frogs, fish and flies.
TL;DR: Two types of genes activated by neural inducers have been identified, those that lead to the activation of proneural genes and those that limit the activity of these genes to specific domains in the neural plate.
Journal ArticleDOI
Early evolution of a homeobox gene: the parahox gene Gsx in the Cnidaria and the Bilateria
TL;DR: A molecular phylogenetic analysis of cnox2, the best studied homeobox gene from the phylum Cnidaria, is performed, decisively favors the hypothesis that cnox 2 is orthologous to the gsx gene of Bilateria, thereby establishing the existence of this specific homeOBox gene in the eumetazoan stem lineage.
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Neuronal migration and ventral subtype identity in the telencephalon depend on SOX1.
Antigoni Ekonomou,Ilias Kazanis,S Malas,Heather Wood,Pavlos Alifragis,Myrto Denaxa,Domna Karagogeos,Andrew Constanti,Robin Lovell-Badge,Vasso Episkopou +9 more
TL;DR: The requirement for SOX1 at a cellular level is addressed, revealing both the nature and timing of the defect and suggesting that other SOXB1 members showing expression in specific neuronal populations are likely to play continuous roles from the establishment of precursors to their final differentiation.
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