Gsh-1: a novel murine homeobox gene expressed in the central nervous system
M. Todd Valerius,Hung Li,Jeffrey L. Stock,Michael Weinstein,Satbir Kaur,Gurparkash Singh,S. Steven Potter +6 more
Reads0
Chats0
TLDR
The characterization of Gsh‐1, a novel murine homeobox gene, is reported, with a transcript of approximately 2 kb in size present at embryonic days 10.5 to E13.5 and the consensus DNA ninding site of the GSh‐1 homeoprotein is determined to be GCT/CA/CATTAG/A.Abstract:
We report the characterization of Gsh-1, a novel murine homeobox gene. Northern blot analysis revealed a transcript of approximately 2 kb in size present at embryonic days 10.5, 11.5, and 12.5 of development. The cDNA sequence encoded a proline rich motif, a polyalanine tract, and a homeodomain with strong homology to those encoded by the clustered Hox genes. The Gsh-1 expression pattern was determined for days E8.5 to E13.5 by whole mount and serial section in situ hybridizations. Gsh-1 transcription was restricted to the central nervous system. Expression is present in the neural tube and hindbrain as two continuous, bilaterally symmetrical stripes within neural epithelial tissue. In the mesencephalon, expression is seen as a band across the most anterior portion. There is also diencephalon expression in the anlagen of the thalamus and the hypothalamus as well as in the optic stalk, optic recess, and the ganglionic eminence. Moreover, through the use of fusion proteins containing the Gsh-1 homeodomain, we have determined the consensus DNA ninding site of the Gsh-1 homeoprotein to be GCT/CA/CATTAG/A. ©1995 Wiley-Liss, Inc.read more
Citations
More filters
Journal ArticleDOI
Lampreys, the jawless vertebrates, contain only two ParaHox gene clusters
Huixian Zhang,Vydianathan Ravi,Boon-Hui Tay,Sumanty Tohari,Nisha E. Pillai,Aravind Prasad,Qiang Lin,Sydney Brenner,Byrappa Venkatesh +8 more
TL;DR: It is shown that lampreys contain only two ParaHox gene clusters compared with four ParAHox loci in most jawed vertebrates, and it is likely that the lamprey Pdxα plays a crucial role in pancreas specification and insulin production similar to the Pdx of jawing vertebrates.
Journal ArticleDOI
Multiple binding sites for nuclear proteins of the anterior pituitary are located in the 5′-flanking region of the porcine follicle-stimulating hormone (FSH) β-subunit gene
TL;DR: Data suggest that multiple proteins of the anterior pituitary recognize a specific region of the porcine FSH beta-subunit gene, which is related to gonadotropins, follicle-stimulating hormone, and luteinizing hormone.
Journal ArticleDOI
Genome Duplications as the Basis of Vertebrates’ Evolutionary Success
TL;DR: Modern concepts of the mechanisms and consequences of genomic duplications in vertebrate evolution are considered in the light of recent experimental data and currently proposed models of vertebrate genome evolution.
Journal ArticleDOI
TCF/Lef regulates the Gsx ParaHox gene in central nervous system development in chordates.
TL;DR: The possibility of TCF/Lef not only as a regulator of chordate Gsx, but as a deeply conserved regulatory factor controlling all three ParaHox genes across the Metazoa is discussed.
Journal ArticleDOI
Gsx transcription factors repress Iroquois gene expression.
TL;DR: Data indicate that Gsx factors act as negative regulators of Iroquois gene expression in the amphibian neural plate and support a model in which the GsX proteins promote neuronal differentiation by repressing the expression of known inhibitors of neuronal differentiation such as Iro3.
References
More filters
Book
Manipulating the mouse embryo: A laboratory manual
TL;DR: Here are recorded the tech- niques for preparing, inserting and analysing DNA sequences, for retroviral infection of mice, for production and use of EC and EK cells as vehicles for engineered sequences and for nuclear transplantation - all against a background of the basic procedures required for pro- ducing and handling the em- bryos.
Journal ArticleDOI
An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs
TL;DR: 5'-Noncoding sequences have been compiled from 699 vertebrate mRNAs and GCCA/GCCATGG emerges as the consensus sequence for initiation of translation in vertebrates.
Journal ArticleDOI
A gene complex controlling segmentation in Drosophila.
TL;DR: The wild-type and mutant segmentation patterns are consistent with an antero-posterior gradient in repressor concentration along the embryo and a proximo-distal gradient along the chromosome in the affinities for repressor of each gene's cis-regulatory element.
Journal ArticleDOI
Transcriptional regulation in mammalian cells by sequence-specific DNA binding proteins
Pamela J. Mitchell,Robert Tjian +1 more
TL;DR: This review summarizes recent studies that define structural domains for DNA binding and transcriptional activation functions in sequence-specific transcription factors in mammalian DNA binding transcription factors.
Journal ArticleDOI
Homeobox genes and axial patterning
William McGinnis,Robb Krumlauf +1 more
TL;DR: Over the past seven years, the term “homeodomain” has evolved to define a class of protein domains that have recognizable similarity to a 60 amino acid motif originally recognized in three Drosophila homeotic and segmentation proteins.