Gsh-1: a novel murine homeobox gene expressed in the central nervous system
M. Todd Valerius,Hung Li,Jeffrey L. Stock,Michael Weinstein,Satbir Kaur,Gurparkash Singh,S. Steven Potter +6 more
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TLDR
The characterization of Gsh‐1, a novel murine homeobox gene, is reported, with a transcript of approximately 2 kb in size present at embryonic days 10.5 to E13.5 and the consensus DNA ninding site of the GSh‐1 homeoprotein is determined to be GCT/CA/CATTAG/A.Abstract:
We report the characterization of Gsh-1, a novel murine homeobox gene. Northern blot analysis revealed a transcript of approximately 2 kb in size present at embryonic days 10.5, 11.5, and 12.5 of development. The cDNA sequence encoded a proline rich motif, a polyalanine tract, and a homeodomain with strong homology to those encoded by the clustered Hox genes. The Gsh-1 expression pattern was determined for days E8.5 to E13.5 by whole mount and serial section in situ hybridizations. Gsh-1 transcription was restricted to the central nervous system. Expression is present in the neural tube and hindbrain as two continuous, bilaterally symmetrical stripes within neural epithelial tissue. In the mesencephalon, expression is seen as a band across the most anterior portion. There is also diencephalon expression in the anlagen of the thalamus and the hypothalamus as well as in the optic stalk, optic recess, and the ganglionic eminence. Moreover, through the use of fusion proteins containing the Gsh-1 homeodomain, we have determined the consensus DNA ninding site of the Gsh-1 homeoprotein to be GCT/CA/CATTAG/A. ©1995 Wiley-Liss, Inc.read more
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The Sox-domain containing gene Dichaete/fish-hook acts in concert with vnd and ind to regulate cell fate in the Drosophila neuroectoderm.
Guoyan Zhao,James B. Skeath +1 more
TL;DR: It is shown that the Sox-domain-containing gene Dichaete/fish-hook also plays a crucial role to pattern the neuroectoderm along the DV axis, and that DichAete acts in concert with ventral nerve cord defective and intermediate neuroblasts defective to regulate pattern and cell fate in the neuroectederm.
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Gene structure and larval expression of cnox-2Am from the coral Acropora millepora
David C. Hayward,Julian Catmull,John S. Reece-Hoyes,Heinrich Berghammer,Heather Dodd,Steven J. Hann,David J. Miller,Eldon E. Ball +7 more
TL;DR: The pattern of expression of cnox-2Am resembles that of Gsx orthologs in Drosophila and vertebrates in being expressed in a spatially restricted portion of the nervous system.
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Ascl1 is a required downstream effector of Gsx gene function in the embryonic mouse telencephalon.
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Gene Maps and Related Histogenetic Domains in the Forebrain and Midbrain
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Breakup of a homeobox cluster after genome duplication in teleosts
TL;DR: It is proposed that this homeobox gene cluster is held together in chordates by the existence of interdigitated control regions that could be separated after locus duplication in the teleost fish.
References
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TL;DR: Over the past seven years, the term “homeodomain” has evolved to define a class of protein domains that have recognizable similarity to a 60 amino acid motif originally recognized in three Drosophila homeotic and segmentation proteins.