Gsh-1: a novel murine homeobox gene expressed in the central nervous system
M. Todd Valerius,Hung Li,Jeffrey L. Stock,Michael Weinstein,Satbir Kaur,Gurparkash Singh,S. Steven Potter +6 more
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TLDR
The characterization of Gsh‐1, a novel murine homeobox gene, is reported, with a transcript of approximately 2 kb in size present at embryonic days 10.5 to E13.5 and the consensus DNA ninding site of the GSh‐1 homeoprotein is determined to be GCT/CA/CATTAG/A.Abstract:
We report the characterization of Gsh-1, a novel murine homeobox gene. Northern blot analysis revealed a transcript of approximately 2 kb in size present at embryonic days 10.5, 11.5, and 12.5 of development. The cDNA sequence encoded a proline rich motif, a polyalanine tract, and a homeodomain with strong homology to those encoded by the clustered Hox genes. The Gsh-1 expression pattern was determined for days E8.5 to E13.5 by whole mount and serial section in situ hybridizations. Gsh-1 transcription was restricted to the central nervous system. Expression is present in the neural tube and hindbrain as two continuous, bilaterally symmetrical stripes within neural epithelial tissue. In the mesencephalon, expression is seen as a band across the most anterior portion. There is also diencephalon expression in the anlagen of the thalamus and the hypothalamus as well as in the optic stalk, optic recess, and the ganglionic eminence. Moreover, through the use of fusion proteins containing the Gsh-1 homeodomain, we have determined the consensus DNA ninding site of the Gsh-1 homeoprotein to be GCT/CA/CATTAG/A. ©1995 Wiley-Liss, Inc.read more
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Journal ArticleDOI
Agenesis of the putamen and globus pallidus caused by recessive mutations in the homeobox gene GSX2
Roberta De Mori,Mariasavina Severino,Maria Margherita Mancardi,Danila Anello,Silvia Tardivo,Tommaso Biagini,Valeria Capra,Antonella Casella,Cristina Cereda,Brett Copeland,Stella Gagliardi,Alessandra Gamucci,Monia Ginevrino,Barbara Illi,Elisa Lorefice,Damir Musaev,Valentina Stanley,Alessia Micalizzi,Joseph G. Gleeson,Tommaso Mazza,Andrea Rossi,Enza Maria Valente +21 more
TL;DR: A novel syndrome of severe dystonic tetraparesis and intellectual impairment, with hypo/agenesis of the basal ganglia, caused by recessive mutations in GSX2, a homeobox gene expressed in ganglionic eminences and essential for basalganglia development.
Journal ArticleDOI
Genetic Regulation of Vertebrate Forebrain Development by Homeobox Genes
Ryan Leung,Ankita M. George,Enola M. Roussel,Maree C. Faux,Jeffrey T. Wigle,David D. Eisenstat +5 more
TL;DR: This comprehensive review outlines the integral role of key homeobox transcription factors and their target genes on forebrain development, focused primarily on the telencephalon, and links of these transcription factors to human diseases, such as neurodevelopmental disorders and brain tumors.
Journal ArticleDOI
Maintenance of neural stem cell regional identity in culture.
TL;DR: A V-SVZ monolayer culture system that retains regional gene expression and neurogenic potential of NSCs from the dorsal and ventral V-VZ retains key aspects of their in vivo regional identity in culture, providing new experimental opportunities for understanding how such developmental patterns are established and maintained during development.
Dissertation
Nature or nurture?: testing the correlation between stress tolerance and genotype in the coral Acropora millepora on the Great Barrier Reef
TL;DR: A spatial Bayesian belief network model was developed to predict GBR-wide spatial distributions of allele frequencies at the antioxidant capacity locus C29226S281 and validated the two genetic markers as true quantitative trait loci for anti-oxidant capacity in the common coral, A. millepora.
Journal ArticleDOI
A 66-bp deletion in growth hormone releasing hormone gene 5′-flanking region with largemouth bass recessive embryonic lethal
TL;DR: The results hint that the 66-bp deletion site in GHRH 5'-flanking sequence significantly affects the livability in largemouth bass embryonic development.
References
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