Gsh-1: a novel murine homeobox gene expressed in the central nervous system
M. Todd Valerius,Hung Li,Jeffrey L. Stock,Michael Weinstein,Satbir Kaur,Gurparkash Singh,S. Steven Potter +6 more
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TLDR
The characterization of Gsh‐1, a novel murine homeobox gene, is reported, with a transcript of approximately 2 kb in size present at embryonic days 10.5 to E13.5 and the consensus DNA ninding site of the GSh‐1 homeoprotein is determined to be GCT/CA/CATTAG/A.Abstract:
We report the characterization of Gsh-1, a novel murine homeobox gene. Northern blot analysis revealed a transcript of approximately 2 kb in size present at embryonic days 10.5, 11.5, and 12.5 of development. The cDNA sequence encoded a proline rich motif, a polyalanine tract, and a homeodomain with strong homology to those encoded by the clustered Hox genes. The Gsh-1 expression pattern was determined for days E8.5 to E13.5 by whole mount and serial section in situ hybridizations. Gsh-1 transcription was restricted to the central nervous system. Expression is present in the neural tube and hindbrain as two continuous, bilaterally symmetrical stripes within neural epithelial tissue. In the mesencephalon, expression is seen as a band across the most anterior portion. There is also diencephalon expression in the anlagen of the thalamus and the hypothalamus as well as in the optic stalk, optic recess, and the ganglionic eminence. Moreover, through the use of fusion proteins containing the Gsh-1 homeodomain, we have determined the consensus DNA ninding site of the Gsh-1 homeoprotein to be GCT/CA/CATTAG/A. ©1995 Wiley-Liss, Inc.read more
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Identification of an upstream regulatory element reveals a novel requirement for Ind activity in maintaining ind expression.
TL;DR: It is concluded that Ind can act as a transcriptional activator, and that positive autoregulation of Ind is a mechanism for persistent ind expression within the developing embryonic nervous system.
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A cis-element in the Notch1 locus is involved in the regulation of gene expression in interneuron progenitors.
Evangeline Tzatzalos,Shannon M. Smith,Sung Tae Doh,Hailing Hao,Ying Li,Alson Wu,Martin Grumet,Li Cai +7 more
TL;DR: It is reported that a novel evolutionarily conserved cis-element in the second intron of the Notch1 locus plays an important role in regulating gene expression in interneuron progenitors and is restricted to neural progenitor cells in the ganglionic eminence that are fated to differentiate into GABAergic interneurons of the neocortex.
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Cloning and functional analysis of hypothalamic homeobox gene Bsx1a and its isoform, Bsx1b.
Hui-Yi Chu,Akihira Ohtoshi +1 more
TL;DR: Molecular analyses of a newly identified murine homeobox gene, Bsx/Bsx1a, that is expressed in the developing and postnatal hypothalamus are reported, demonstrating that BSX1A is a DNA binding protein and a transcriptional activator and proposing that alternative RNA splicing is involved in hypothalamic development/function.
Journal ArticleDOI
Conserved Gsx2/Ind homeodomain monomer versus homodimer DNA binding defines regulatory outcomes in flies and mice
Joseph Salomone,Shenyue Qin,Temesgen D. Fufa,Brittany Cain,Edward Farrow,Bin Guan,Robert B. Hufnagel,Masato Nakafuku,Masato Nakafuku,Hee-Woong Lim,Kenneth Campbell,Kenneth Campbell,Brian Gebelein,Brian Gebelein +13 more
TL;DR: In this paper, the mouse and fly homeodomain transcription factors unexpectedly gain DNA binding specificity by forming cooperative homodimers on precisely spaced and oriented DNA sites, which can increase target specificity and alter regulatory outcomes.
Journal ArticleDOI
Pdx1-related homeodomain transcription factors are distinctly expressed in mouse adult pancreatic islets.
TL;DR: This is the first report that locates ParaHox genes other than Pdx1 and Cdx2/3 in a place as to be involved in the pancreatic transcriptional regulatory networks, potentially regulating glucagon-insulin homeostasis.
References
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