Gsh-1: a novel murine homeobox gene expressed in the central nervous system
M. Todd Valerius,Hung Li,Jeffrey L. Stock,Michael Weinstein,Satbir Kaur,Gurparkash Singh,S. Steven Potter +6 more
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TLDR
The characterization of Gsh‐1, a novel murine homeobox gene, is reported, with a transcript of approximately 2 kb in size present at embryonic days 10.5 to E13.5 and the consensus DNA ninding site of the GSh‐1 homeoprotein is determined to be GCT/CA/CATTAG/A.Abstract:
We report the characterization of Gsh-1, a novel murine homeobox gene. Northern blot analysis revealed a transcript of approximately 2 kb in size present at embryonic days 10.5, 11.5, and 12.5 of development. The cDNA sequence encoded a proline rich motif, a polyalanine tract, and a homeodomain with strong homology to those encoded by the clustered Hox genes. The Gsh-1 expression pattern was determined for days E8.5 to E13.5 by whole mount and serial section in situ hybridizations. Gsh-1 transcription was restricted to the central nervous system. Expression is present in the neural tube and hindbrain as two continuous, bilaterally symmetrical stripes within neural epithelial tissue. In the mesencephalon, expression is seen as a band across the most anterior portion. There is also diencephalon expression in the anlagen of the thalamus and the hypothalamus as well as in the optic stalk, optic recess, and the ganglionic eminence. Moreover, through the use of fusion proteins containing the Gsh-1 homeodomain, we have determined the consensus DNA ninding site of the Gsh-1 homeoprotein to be GCT/CA/CATTAG/A. ©1995 Wiley-Liss, Inc.read more
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Gsh2 and Pax6 play complementary roles in dorsoventral patterning of the mammalian telencephalon
TL;DR: Evidence is presented that the progenitor and postmitotic cells flanking the pallial/subpallial boundary (PSB) in the embryonic mouse can be subdivided into multiple regions that express unique combinations of transcription factors.
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Genetic control of dorsal-ventral identity in the telencephalon: opposing roles for Pax6 and Gsh2.
TL;DR: Results demonstrate that Pax6 and Gsh2 govern cortical and striatal development by regulating genetically opposing programs that control the expression of each other as well as the regionally expressed developmental regulators Mash1, the neurogenins and Dlx genes in telencephalic progenitors.
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Multiple Dorsoventral Origins of Oligodendrocyte Generation in the Spinal Cord and Hindbrain
TL;DR: It is proposed that oligodendrocytes derive from several distinct positional origins and that the activation of Olig1/2 at different positions is controlled by distinct genetic programs.
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Ventral neural patterning by Nkx homeobox genes: Nkx6.1 controls somatic motor neuron and ventral interneuron fates
Maike Sander,Sussan Paydar,Johan Ericson,James Briscoe,Elisabeth Berber,Michael S. German,Thomas M. Jessell,John L.R. Rubenstein +7 more
TL;DR: The patterns of neurogenesis in mice carrying a targeted mutation in Nkx6.1 show a dorsal-to-ventral switch in the identity of progenitors and in the fate of postmitotic neurons, demonstrating the essential functions of NkX6.
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Pax-6 functions in boundary formation and axon guidance in the embryonic mouse forebrain.
TL;DR: It is concluded that Pax-6 plays multiple roles in forebrain patterning, including boundary formation, regionally restricted patterns in the developing brain and eye, neuron specification and axon guidance.
References
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Isolation, structure, and expression of even-skipped: A second pair-rule gene of Drosophila containing a homeo box
TL;DR: The eve gene resembles other pair-rule genes in showing a transient seven stripe zebra pattern during the blastoderm stage, but seven additional stripes arise soon thereafter, which are required for the activation of coincident stripes of engrailed transcripts, leading to the subdivision of the embryo into compartmental and segmental units.
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Hoxb-4 (Hox-2.6) mutant mice show homeotic transformation of a cervical vertebra and defects in the closure of the sternal rudiments
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What determines the specificity of action of Drosophila homeodomain proteins
Shigeo Hayashi,Matthew P. Scott +1 more
TL;DR: The homeobox is a evolutionarily conserved 183 bp DNA sequence motif first found in genes that control the morphogenesis of Drosophila melanogaster.
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The Drosophila segmentation gene runt encodes a novel nuclear regulatory protein that is also expressed in the developing nervous system.
TL;DR: The structure of the segmentation gene runt is presented and the pattern of runt protein expression during embryogenesis is described, indicating that the protein is present throughout embryogenesis and is expressed extensively in the developing central and peripheral nervous system.
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Mesodermal control of neural cell identity: floor plate induction by the notochord
TL;DR: Results provide evidence that a local signal from the notochord induces the functional properties of the floor plate.