Human NLRP1 is a sensor of pathogenic coronavirus 3CL proteases in lung epithelial cells
Rémi Planès,Miriam Pinilla,Karin Santoni,Audrey Hessel,Charlotte Passemar,Kenneth James Lay,P. Paillette,Ana Luiza Chaves Valadão,Kim S. Robinson,Paul Bastard,Nathan Lam,Ricardo Fadrique,Ida Rossi,David Péricat,Salimata Bagayoko,Stephen A. Leon-Icaza,Yoann Rombouts,Eric Perouzel,M Tiraby,Qian Zhang,Pietro Cicuta,Emmanuelle Jouanguy,Olivier Neyrolles,Clare E. Bryant,Andres Floto,Caroline Goujon,Franklin Zhong Lei,Guillaume Martin-Blondel,Stein Silva,Jean-Laurent Casanova,Céline Cougoule,Bruno Reversade,Julien Marcoux,Emmanuel Ravet,Etienne Meunier +34 more
Reads0
Chats0
TLDR
In this article , the authors identify the NLRP1 inflammasome as a sensor of SARS-CoV-2 infection in lung epithelia and demonstrate that 3CL proteases also inactivate the pyroptosis executioner Gasdermin D (GSDMD).Citations
More filters
Journal ArticleDOI
The human inflammasomes.
TL;DR: Recently, the field has made notable progress in characterizing human-specific inflammasomes and their regulation mechanisms, including an expansion of inflammome biology to adaptive immune cells as discussed by the authors .
Journal ArticleDOI
Pyroptotic cell death in SARS-CoV-2 infection: revealing its roles during the immunopathogenesis of COVID-19
TL;DR: An overview of the interplay between SARS-CoV-2 infection and pyroptosis is presented and recent research advances in the understanding of the mechanisms responsible for the implication of the pyroPTosis pathways in COVID-19 pathogenesis are highlighted.
Journal ArticleDOI
Recent insights into innate immune nucleic acid sensing during viral infection.
Coralie Guy,Andrew G. Bowie +1 more
TL;DR: In this paper , the authors reveal that viruses also induce the release of host nucleic acid from the nucleus and mitochondria into the cytosol, which boosts the activation of antiviral PRRs.
Journal ArticleDOI
An epithelial-immune circuit amplifies inflammasome and IL-6 responses to SARS-CoV-2
Katherine C. Barnett,Yuying Xie,Takanori Asakura,Dingka Song,Kaixin Liang,Sharon Taft-Benz,Hai-Song Guo,Shuangshuang Yang,Kenichi Okuda,Rodney C. Gilmore,Jennifer F. Loome,Thomas H. Oguin,Gregory D. Sempowski,Scott H. Randell,Mark T. Heise,Yunxuan Lei,Richard C. Boucher,Jenny P.-Y. Ting +17 more
TL;DR: In this paper , the authors investigated the underlying mechanisms of IL-1β and IL-6 release from primary human airway epithelia (HAE) after SARS-CoV-2 infection in severe COVID-19.
Journal ArticleDOI
Antiviral and Anti-Inflammatory Activities of Fluoxetine in a SARS-CoV-2 Infection Mouse Model
David Péricat,Stephen A. Leon-Icaza,Marina Sánchez Rico,Christiane Mühle,Iulia Zoicas,Fabian Schumacher,Rémi Planès,Raoul Mazars,Germain Gros,Alexander Carpinteiro,Katrin Anne Becker,Jacques Izopet,Nathalie Strub-Wourgaft,Peter Sjö,Olivier Neyrolles,Burkhard Kleuser,Frédéric Limosin,Erich Gulbins,Johannes Kornhuber,Etienne Meunier,Nicolas Hoertel,Céline Cougoule +21 more
TL;DR: In this article , the potential antiviral and anti-inflammatory activities of fluoxetine in a K18-hACE2 mouse model of SARS-CoV-2 infection, and against variants of concern in vitro, were evaluated.
References
More filters
Journal ArticleDOI
A simple method of estimating fifty per cent endpoints
Lowell J. Reed,H. Muench +1 more
Journal ArticleDOI
Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR.
Victor M. Corman,Olfert Landt,Marco Kaiser,Richard Molenkamp,Adam Meijer,Daniel K.W. Chu,Tobias Bleicker,Sebastian Brünink,Julia Schneider,Marie Luisa Schmidt,Daphne G.J.C. Mulders,Bart L. Haagmans,Bas van der Veer,Sharon van den Brink,Lisa Wijsman,Gabriel Goderski,Jean Louis Romette,Joanna Ellis,Maria Zambon,Malik Peiris,Herman Goossens,Chantal B.E.M. Reusken,Marion Koopmans,Christian Drosten +23 more
TL;DR: A validated diagnostic workflow for 2019-nCoV is presented, its design relying on close genetic relatedness of 2019- nCoV with SARS coronavirus, making use of synthetic nucleic acid technology.
Journal ArticleDOI
Improved vectors and genome-wide libraries for CRISPR screening.
TL;DR: In this paper, Zhang et al. used a Genome-scale CRISPR Knock-Out (GeCKO) library to identify loss-of-function mutations in a melanoma model.
Journal ArticleDOI
Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death
Jianjin Shi,Yue Zhao,Kun Wang,Xuyan Shi,Wang Yue,Huanwei Huang,Yinghua Zhuang,Tao Cai,Fengchao Wang,Feng Shao +9 more
TL;DR: Gasdermin D (Gsdmd) is identified by genome-wide clustered regularly interspaced palindromic repeat-Cas9 nuclease screens of caspase-11- and caspasing-1-mediated pyroptosis in mouse bone marrow macrophages to offer insight into inflammasome-mediated immunity/diseases and change the understanding of pyroPTosis and programmed necrosis.
Journal ArticleDOI
The trinity of COVID-19: immunity, inflammation and intervention.
Matthew Zirui Tay,Chek Meng Poh,Laurent Rénia,Laurent Rénia,Paul A. MacAry,Lisa F. P. Ng,Lisa F. P. Ng,Lisa F. P. Ng +7 more
TL;DR: The interaction of SARS-CoV-2 with the immune system and the subsequent contribution of dysfunctional immune responses to disease progression is described and the implications of these approaches for potential therapeutic interventions that target viral infection and/or immunoregulation are highlighted.