Integrative Single-Cell RNA-Seq and ATAC-Seq Analysis of Human Developmental Hematopoiesis
Anna Maria Ranzoni,Andrea Tangherloni,Andrea Tangherloni,Andrea Tangherloni,Ivan Berest,Simone Giovanni Riva,Simone Giovanni Riva,Simone Giovanni Riva,Brynelle Myers,Paulina M. Strzelecka,Jiarui Xu,Jiarui Xu,Jiarui Xu,Elisa Panada,Elisa Panada,Irina Mohorianu,Judith B. Zaugg,Ana Cvejic,Ana Cvejic,Ana Cvejic +19 more
TLDR
In this paper, the authors applied single-cell RNA sequencing (scRNA-seq) and single cell assay for transposase-accessible chromatin sequencing to over 8,000 human immunophenotypic blood cells from fetal liver and bone marrow.About:
This article is published in Cell Stem Cell.The article was published on 2021-03-04 and is currently open access. It has received 122 citations till now. The article focuses on the topics: ATAC-seq & Chromatin.read more
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Journal ArticleDOI
Evaluation of Ranzoni et al.: Integrative Single-Cell RNA-Seq and ATAC-Seq Analysis of Human Developmental Hematopoiesis.
TL;DR: In this paper, a peer review process for integrated single-cell RNA-seq and ATAC-Seq analysis of human developmental Hematopoiesis is presented. But this process is restricted to single cell RNA sequencing.
Journal ArticleDOI
High-throughput methods for the analysis of transcription factors and chromatin modifications: Low input, single cell and spatial genomic technologies.
TL;DR: In this article , a set of approaches progressively pushing the limits of epigenetic profiling towards single cells are presented. But the authors focus on single-cell and spatial genomic applications and do not discuss the benefits of using multiple TFs or histone modifications in parallel.
Journal ArticleDOI
Temporary serine protease inhibition and the role of SPINK2 in human bone marrow
Vincenza Barresi,Virginia Di Bella,Luca Lo Nigro,Anna Provvidenza Privitera,Paola Bonaccorso,Chiara Scuderi,Daniele F. Condorelli +6 more
TL;DR: In this paper , the SPINK2 degradation constant was determined and a mathematical relationship was derived to predict the zone of inhibited target protease activity surrounding SPINK-secreting HSPCs.
Posted ContentDOI
Postnatal lymph node expansion of stromal progenitors towards reticular and CD34+ stromal cell subsets is determined by distinct transcriptional programs
Joern Pezoldt,Carolin Wiechrs,Maria Litovchenko,Marjan Biočanin,Mangge Zou,Katarzyna M. Sitnik,Michael Beckstette,Wanze Chen,Vincent Gardeux,Stefan Floess,Maria Ebel,Julie Russeil,Panagiota Arampatzi,Ehsan Vafardanejad,Antoine-Emmanuel Saliba,Bart Deplancke,Jochen Huehn +16 more
TL;DR: The tissue-specific epigenomic DNA accessibility and CpG methylation landscape of LN non-endothelial SCs is dissected and a microbiota-independent core epigenomic signature is identified, representing a valuable resource to identify core transcriptional regulators that impinge on the developing mLN early in life, thereby shaping long-lasting intestinal adaptive immune responses.
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