Integrative Single-Cell RNA-Seq and ATAC-Seq Analysis of Human Developmental Hematopoiesis
Anna Maria Ranzoni,Andrea Tangherloni,Andrea Tangherloni,Andrea Tangherloni,Ivan Berest,Simone Giovanni Riva,Simone Giovanni Riva,Simone Giovanni Riva,Brynelle Myers,Paulina M. Strzelecka,Jiarui Xu,Jiarui Xu,Jiarui Xu,Elisa Panada,Elisa Panada,Irina Mohorianu,Judith B. Zaugg,Ana Cvejic,Ana Cvejic,Ana Cvejic +19 more
TLDR
In this paper, the authors applied single-cell RNA sequencing (scRNA-seq) and single cell assay for transposase-accessible chromatin sequencing to over 8,000 human immunophenotypic blood cells from fetal liver and bone marrow.About:
This article is published in Cell Stem Cell.The article was published on 2021-03-04 and is currently open access. It has received 122 citations till now. The article focuses on the topics: ATAC-seq & Chromatin.read more
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A plate-based single-cell ATAC-seq workflow for fast and robust profiling of chromatin accessibility
TL;DR: In this article, a plate-based ATAC-seq assay was proposed for single-cell chromatin accessibility profiling based on the assay for transposase-accessible chromatin using sequencing (ATACseq).
Journal ArticleDOI
Understanding mast cell heterogeneity at single cell resolution
Gökhan Cildir,Kwok Ho Yip,Harshita Pant,Harshita Pant,Vinay Tergaonkar,Vinay Tergaonkar,Vinay Tergaonkar,Angel F. Lopez,Angel F. Lopez,Damon J. Tumes +9 more
TL;DR: A review of the current understanding of MC development and heterogeneity is provided in this article, where the authors discuss new insights gained from single-cell-based studies that may lead to future research directions and therapeutic opportunities.
Journal ArticleDOI
Multi-omic single-cell velocity models epigenome–transcriptome interactions and improves cell fate prediction
TL;DR: MultiVelo, a differential equation model of gene expression that extends the RNA velocity framework to incorporate epigenomic data, uses a probabilistic latent variable model to estimate the switch time and rate parameters of chromatin accessibility and gene expression and improves the accuracy of cell fate prediction compared to velocity estimates from RNA only.
Journal ArticleDOI
Single-cell RNA sequencing to track novel perspectives in HSC heterogeneity
Pan Zhang,Xiang Li,Chengwei Pan,Xinmin Zheng,Bohan Hu,Ruiheng Xie,Jialu Hu,Xuequn Shang,Hui Yang +8 more
TL;DR: In this paper , the authors discuss how single-cell RNA sequencing (scRNA-seq) technologies contribute to tracing origin and lineage commitment of HSCs, profiling the bone marrow microenvironment and providing high-resolution dissection of malignant hematopoiesis.
Journal ArticleDOI
A specialized bone marrow microenvironment for fetal haematopoiesis
TL;DR: In this paper , a single cell RNA sequencing was performed to identify Wnt2 as arterial endothelial cells (AEC)-derived signal that activates β-catenin-dependent proliferation of fetal HSPCs.
References
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