Intestinal Dysbiosis and Depletion of Butyrogenic Bacteria in Clostridium difficile Infection and Nosocomial Diarrhea
Reads0
Chats0
TLDR
In this paper, the authors used high-density Roche 454 pyrosequencing to survey the distal gut microbiota for 39 individuals with CDI, 36 subjects with C. difficile-negative nosocomial diarrhea (CDN), and 40 healthy control subjects.Abstract:
Clostridium difficile infection (CDI) causes nearly half a million cases of diarrhea and colitis in the United States each year. Although the importance of the gut microbiota in C. difficile pathogenesis is well recognized, components of the human gut flora critical for colonization resistance are not known. Culture-independent high-density Roche 454 pyrosequencing was used to survey the distal gut microbiota for 39 individuals with CDI, 36 subjects with C. difficile-negative nosocomial diarrhea (CDN), and 40 healthy control subjects. A total of 526,071 partial 16S rRNA sequence reads of the V1 to V3 regions were aligned with 16S databases, identifying 3,531 bacterial phylotypes from 115 fecal samples. Genomic analysis revealed significant alterations of organism lineages in both the CDI and CDN groups, which were accompanied by marked decreases in microbial diversity and species richness driven primarily by a paucity of phylotypes within the Firmicutes phylum. Normally abundant gut commensal organisms, including the Ruminococcaceae and Lachnospiraceae families and butyrate-producing C2 to C4 anaerobic fermenters, were significantly depleted in the CDI and CDN groups. These data demonstrate associations between the depletion of Ruminococcaceae, Lachnospiraceae, and butyrogenic bacteria in the gut microbiota and nosocomial diarrhea, including C. difficile infection. Mechanistic studies focusing on the functional roles of these organisms in diarrheal diseases and resistance against C. difficile colonization are warranted.read more
Citations
More filters
Journal ArticleDOI
Intestinal Dysbiosis Associated with Systemic Lupus Erythematosus
Arancha Hevia,Christian Milani,Patricia López,Adriana Cuervo,Silvia Arboleya,Sabrina Duranti,Francesca Turroni,Sonia González,Ana Suárez,Miguel Gueimonde,Marco Ventura,Borja Sánchez,Abelardo Margolles +12 more
TL;DR: This is the first report describing an SLE-associated intestinal dysbiosis, and it contributes to the understanding of the interplay between the intestinal microbiota and the host in autoimmune disorders.
Journal ArticleDOI
Antibiotics as deep modulators of gut microbiota: between good and evil
TL;DR: The potential of antibiotics in the development of major non-communicable disorders associated with the alteration of gut microbiota and on newly discovered therapeutic avenues of antibiotics beyond the cure of infectious diseases are addressed.
Journal ArticleDOI
Fecal microbiota transplant from a rational stool donor improves hepatic encephalopathy: A randomized clinical trial
Jasmohan S. Bajaj,Zain Kassam,Andrew Fagan,Edith Gavis,Eric J. Liu,I. Jane Cox,Raffi Kheradman,Douglas M. Heuman,Jessica Wang,Thomas Gurry,Roger Williams,Masoumeh Sikaroodi,Michael Fuchs,Eric J. Alm,Binu John,Leroy R. Thacker,Antonio Riva,Mark Smith,Simon D. Taylor-Robinson,Patrick M. Gillevet +19 more
TL;DR: FMT from a rationally selected donor reduced hospitalizations, improved cognition, and dysbiosis in cirrhosis with recurrent HE, and increased diversity and beneficial taxa.
Journal ArticleDOI
Imbalance of gut microbiome and intestinal epithelial barrier dysfunction in patients with high blood pressure.
Seungbum Kim,Ruby Goel,Ashok V. Kumar,Yanfei Qi,Gil Lobaton,Koji Hosaka,Mohammed Mohammed,Eileen M. Handberg,Elaine M. Richards,Carl J. Pepine,Mohan K. Raizada +10 more
TL;DR: The hypotheses that high blood pressure patients have distinct gut microbiomes and that gut-epithelial barrier function markers and microbiome composition could predict systolic BP (SBP) were tested, supporting the conclusion that intestinal barrier dysfunction and microbiome function are linked to HTN in humans.
Journal ArticleDOI
Recovery of the Gut Microbiome following Fecal Microbiota Transplantation
Anna M. Seekatz,Johannes Aas,Charles E. Gessert,Timothy A. Rubin,Daniel M. Saman,Johan S. Bakken,Vincent B. Young +6 more
TL;DR: In this article, the authors investigated changes in the fecal microbiota structure following FMT in patients with recurrent C. difficile infection, and imputed a hypothetical func- tional profile based on the 16S rRNA profile using a predictive metagenomic tool.
References
More filters
Journal ArticleDOI
Metagenomic biomarker discovery and explanation
Nicola Segata,Jacques Izard,Jacques Izard,Levi Waldron,Dirk Gevers,Larisa Miropolsky,Wendy S. Garrett,Curtis Huttenhower +7 more
TL;DR: A new method for metagenomic biomarker discovery is described and validates by way of class comparison, tests of biological consistency and effect size estimation to address the challenge of finding organisms, genes, or pathways that consistently explain the differences between two or more microbial communities.
Journal ArticleDOI
SILVA: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARB
Elmar Pruesse,Christian Quast,Katrin Knittel,Bernhard M. Fuchs,Wolfgang Ludwig,Jörg Peplies,Frank Oliver Glöckner +6 more
TL;DR: SILVA (from Latin silva, forest), was implemented to provide a central comprehensive web resource for up to date, quality controlled databases of aligned rRNA sequences from the Bacteria, Archaea and Eukarya domains.
Journal ArticleDOI
The human microbiome project.
Peter J. Turnbaugh,Ruth E. Ley,Micah Hamady,Claire M. Fraser-Liggett,Rob Knight,Jeffrey I. Gordon +5 more
TL;DR: A strategy to understand the microbial components of the human genetic and metabolic landscape and how they contribute to normal physiology and predisposition to disease.
Journal ArticleDOI
Duodenal Infusion of Donor Feces for Recurrent Clostridium difficile
Els van Nood,A. Vrieze,Max Nieuwdorp,Susana Fuentes,Erwin G. Zoetendal,Willem M. de Vos,Caroline E. Visser,Ed J. Kuijper,Peter Speelman,Josbert J. Keller +9 more
TL;DR: The infusion of donor feces was significantly more effective for the treatment of recurrent C. difficile infection than the use of vancomycin and patients showed increased fecal bacterial diversity, similar to that in healthy donors, with an increase in Bacteroidetes species and clostridium clusters IV and XIVa and a decrease in Proteobacteria species.
Journal ArticleDOI
Clinical Practice Guidelines for Clostridium difficile Infection in Adults: 2010 Update by the Society for Healthcare Epidemiology of America (SHEA) and the Infectious Diseases Society of America (IDSA)
Stuart H. Cohen,Dale N. Gerding,Stuart Johnson,Ciaran P. Kelly,Vivian G. Loo,L. Clifford McDonald,Jacques Pépin,Mark H. Wilcox +7 more
TL;DR: This guideline updates recommendations regarding epidemiology, diagnosis, treatment, and infection control and environmental management of Clostridium difficile.
Related Papers (5)
Precision microbiome reconstitution restores bile acid mediated resistance to Clostridium difficile.
Charlie G. Buffie,Vanni Bucci,Vanni Bucci,Richard R. Stein,Peter T. McKenney,Lilan Ling,Asia Gobourne,Daniel No,Hui Liu,Melissa A. Kinnebrew,Agnes Viale,Eric R. Littmann,Marcel R.M. van den Brink,Marcel R.M. van den Brink,Robert R. Jenq,Ying Taur,Chris Sander,Justin R. Cross,Nora C. Toussaint,Nora C. Toussaint,Joao B. Xavier,Joao B. Xavier,Eric G. Pamer,Eric G. Pamer +23 more
QIIME allows analysis of high-throughput community sequencing data.
J. Gregory Caporaso,Justin Kuczynski,Jesse Stombaugh,Kyle Bittinger,Frederic D. Bushman,Elizabeth K. Costello,Noah Fierer,Antonio Gonzalez Peña,Julia K. Goodrich,Jeffrey I. Gordon,Gavin A. Huttley,Scott T. Kelley,Dan Knights,Jeremy E. Koenig,Ruth E. Ley,Catherine A. Lozupone,Daniel McDonald,Brian D. Muegge,Meg Pirrung,Jens Reeder,Joel Sevinsky,Peter J. Turnbaugh,William A. Walters,Jeremy Widmann,Tanya Yatsunenko,Jesse R. Zaneveld,Rob Knight,Rob Knight +27 more