Intracellular pathways for lignin catabolism in white-rot fungi.
Carlos del Cerro,Erika Erickson,Tao Dong,Allison R. Wong,Elizabeth K. Eder,Samuel O. Purvine,Hugh D. Mitchell,Karl K. Weitz,Lye Meng Markillie,Meagan C. Burnet,David W. Hoyt,Rosalie K. Chu,Jan Fang Cheng,Kelsey J. Ramirez,Rui Katahira,Wei Xiong,Michael E. Himmel,Venkataramanan Subramanian,Jeffrey G. Linger,Davinia Salvachúa +19 more
TLDR
In this paper, the authors employ 13C-isotope labeling, systems biology approaches, and in vitro enzyme assays to demonstrate that two white-rot fungi, Trametes versicolor and Gelatoporia subvermispora, funnel carbon from lignin-derived aromatic compounds into central carbon metabolism via intracellular catabolic pathways.Abstract:
Lignin is a biopolymer found in plant cell walls that accounts for 30% of the organic carbon in the biosphere. White-rot fungi (WRF) are considered the most efficient organisms at degrading lignin in nature. While lignin depolymerization by WRF has been extensively studied, the possibility that WRF are able to utilize lignin as a carbon source is still a matter of controversy. Here, we employ 13C-isotope labeling, systems biology approaches, and in vitro enzyme assays to demonstrate that two WRF, Trametes versicolor and Gelatoporia subvermispora, funnel carbon from lignin-derived aromatic compounds into central carbon metabolism via intracellular catabolic pathways. These results provide insights into global carbon cycling in soil ecosystems and furthermore establish a foundation for employing WRF in simultaneous lignin depolymerization and bioconversion to bioproducts-a key step toward enabling a sustainable bioeconomy.read more
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Critical enzyme reactions in aromatic catabolism for microbial lignin conversion
Erika Erickson,Alissa Bleem,Eugene Kuatsjah,Allison Z. Werner,Jennifer L. DuBois,John McGeehan,Lindsay D. Eltis,Gregg T. Beckham +7 more
TL;DR: The known enzymatic mechanisms for aromatic O-demethylation, decarboxylation and hydroxylation for lignin valorization via biological funnelling are reviewed, highlighting opportunities at the intersection of biochemistry, enzyme engineering and metabolic engineering for applications in the expanding field of microbial lignIn Valorization.
Journal ArticleDOI
Valorization of lignin components into gallate by integrated biological hydroxylation, O-demethylation, and aryl side-chain oxidation.
TL;DR: In this article, an efficient biocatalyst was developed to selectively produce gallate from lignin components by integrating them into a single product, which is a promising way to upgrade Lignin.
Journal ArticleDOI
Guiding stars to the field of dreams: Metabolically engineered pathways and microbial platforms for a sustainable lignin-based industry
TL;DR: The natural pathway atlas as discussed by the authors provides a guiding star for metabolic engineers to breed designed cell factories and efficiently upgrade this global waste stream, which offers a sustainable product portfolio from lignin, comprising bulk and specialty chemicals, biomaterials, and fuels.
Journal ArticleDOI
Recent Advances in Synthesis and Degradation of Lignin and Lignin Nanoparticles and Their Emerging Applications in Nanotechnology
Virendra Kumar Yadav,Nitin Gupta,Pankaj Kumar,Marjan Ganjali Dashti,Vineet Tirth,Samreen Heena Khan,Krishna Kumar Yadav,Saiful Islam,Nisha Choudhary,Ali Algahtani,Sweta Parimita Bera,Do Hyeon Kim,Byong-Hun Jeon +12 more
TL;DR: In this article , a review of the recent advances in the biodegradation of lignin in the ecological system is presented, which includes the current approaches, methods for modification, new applications and research for the synthesis of Lignin and LBNs.
Journal ArticleDOI
Guiding stars to the field of dreams: Metabolically engineered pathways and microbial platforms for a sustainable lignin-based industry
TL;DR: The natural pathway atlas as discussed by the authors provides a guiding star for metabolic engineers to breed designed cell factories and efficiently upgrade this global waste stream, in combination with success stories from system metabolic engineering, as reviewed here, promises a sustainable product portfolio from lignin, comprising bulk and specialty chemicals, biomaterials, and fuels.
References
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