Journal ArticleDOI
Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production
Jorge Alonso-Gutierrez,Rossana Chan,Rossana Chan,Tanveer S. Batth,Tanveer S. Batth,Paul D. Adams,Paul D. Adams,Jay D. Keasling,Christopher J. Petzold,Christopher J. Petzold,Taek Soon Lee,Taek Soon Lee +11 more
TLDR
A strain containing all mevalonate pathway genes in a single plasmid produced limonene at titers over 400mg/L from glucose, substantially higher than has been achieved in the past.About:
This article is published in Metabolic Engineering.The article was published on 2013-09-01. It has received 328 citations till now. The article focuses on the topics: Perillyl alcohol & Limonene.read more
Citations
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Bioprospecting For Genes That Confer Biofuel Tolerance To Escherichia Coli Using A Genomic Library Approach
TL;DR: The results suggest that by controlling the expression of appropriate heterologous sigma factors, the authors can greatly increase the searchable genomic space within transgenic libraries, and shows how tolerance genes can improve bacterial growth in the presence of toxic biofuel compounds.
Book ChapterDOI
Microbial Production of Natural Flavors and Fragrances
TL;DR: In this article , the authors highlight important chemicals for flavor and fragrance and their engineered production and highlight the need to produce bio-based products in a renewable manner in order to deal with global warming, with climate changes and significantly decreasing petrochemical resources.
Book ChapterDOI
Synthetic Biology Advances for Enrichment of Bioactive Molecules in Plants
TL;DR: The molecular biology approaches used for production of the secondary metabolites have been summarized and the case studies of the biosynthetic production of high-value biomolecules and the future perspectives of synthetic biology have been discussed.
Journal ArticleDOI
Evaluating the efficacy of genetically engineered Escherichia coli W (ATCC 9637) to produce limonene from industrial sugar beets (Beta vulgaris L.)
Isabel C. Isaac,Stephanie A. Wootton,Tylor J. Johnson,Tylor J. Johnson,Emily L. Baldwin,Liping Gu,Bishnu Karki,Alec N. Williams,Charles Halfmann,Huilan Zhu,Juan M. Vargas-Ramirez,Ruanbao Zhou,Dennis P. Wiesenborn,William R. Gibbons +13 more
TL;DR: In this paper, beet juice from sugar beets was used as a novel feedstock to produce the cyclic hydrocarbon limonene by genetically engineered E. coli W (ATCC 9637).
Journal ArticleDOI
Repetitive genomic insertion of gene-sized dsDNAs by targeting the promoter region of a counter-selectable marker
Jaehwan Jeong,Han Na Seo,Yu Kyung Jung,Jeewon Lee,Gyuri Ryu,Wookjae Lee,Euijin Kwon,Keunsoo Ryoo,Jungyeon Kim,Hwa Young Cho,Kwang Myung Cho,Jin Hwan Park,Duhee Bang +12 more
TL;DR: A genome engineering method enabling the continuous incorporation of gene-sized double-stranded DNAs (dsDNAs) into the Escherichia coli genome by synchronously turning an endogenous counter-selectable marker gene ON and OFF is reported.
References
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Journal ArticleDOI
Studies on transformation of Escherichia coli with plasmids
Douglas Hanahan,Douglas Hanahan +1 more
TL;DR: Competition with both transforming and non-transforming plasmids indicates that each cell is capable of taking up many DNA molecules, and that the establishment of a transformation event is neither helped nor hindered significantly by the presence of multiple plasmid molecules.
Journal ArticleDOI
Skyline: an open source document editor for creating and analyzing targeted proteomics experiments
Brendan MacLean,Daniela M. Tomazela,Nicholas J. Shulman,Matthew C. Chambers,Gregory L. Finney,Barbara Frewen,Randall Kern,David L. Tabb,Daniel C. Liebler,Michael J. MacCoss +9 more
TL;DR: The Skyline user interface simplifies the development of mass spectrometer methods and the analysis of data from targeted proteomics experiments performed using selected reaction monitoring (SRM).
Journal ArticleDOI
Production of the antimalarial drug precursor artemisinic acid in engineered yeast
Dae-Kyun Ro,Eric M. Paradise,Mario Ouellet,Karl Fisher,Karyn L. Newman,John M. Ndungu,Ho Kimberly,Eachus Rachel,Timothy S. Ham,James Kirby,Michelle C. Y. Chang,Sydnor T. Withers,Yoichiro Shiba,Richmond Sarpong,Jay D. Keasling +14 more
TL;DR: The engineering of Saccharomyces cerevisiae to produce high titres (up to 100 mg l-1) of artemisinic acid using an engineered mevalonate pathway, amorphadiene synthase, and a novel cytochrome P450 monooxygenase from A. annua that performs a three-step oxidation of amorpha-4,11-diene to art Artemisinic acid.
Journal ArticleDOI
Analysis of gene control signals by DNA fusion and cloning in Escherichia coli
TL;DR: Plasmid cloning vectors that enable insertion of DNA fragments between the inducible ara (arabinose) promoter and the lac (lactose) structural genes have been constructed and used for the detection and analysis of signals that control gene transcription.
Journal ArticleDOI
Engineering a mevalonate pathway in Escherichia coli for production of terpenoids
TL;DR: The strains developed in this study can serve as platform hosts for the production of any terpenoid compound for which a terpene synthase gene is available, and are the universal precursors to all isoprenoids.
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