Journal ArticleDOI
Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production
Jorge Alonso-Gutierrez,Rossana Chan,Rossana Chan,Tanveer S. Batth,Tanveer S. Batth,Paul D. Adams,Paul D. Adams,Jay D. Keasling,Christopher J. Petzold,Christopher J. Petzold,Taek Soon Lee,Taek Soon Lee +11 more
TLDR
A strain containing all mevalonate pathway genes in a single plasmid produced limonene at titers over 400mg/L from glucose, substantially higher than has been achieved in the past.About:
This article is published in Metabolic Engineering.The article was published on 2013-09-01. It has received 328 citations till now. The article focuses on the topics: Perillyl alcohol & Limonene.read more
Citations
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Journal ArticleDOI
Metabolic engineering and synthetic biology for isoprenoid production in Escherichia coli and Saccharomyces cerevisiae
Govinda R. Navale,Govinda R. Navale,Mahesh S. Dharne,Mahesh S. Dharne,Sandip S. Shinde,Sandip S. Shinde +5 more
TL;DR: In this article, the authors provide a detailed description of various approaches used for engineering of methyl-D-erythritol-4-phosphate (MEP) and mevalonate pathway for synthesizing isoprene units (C5) and ultimate production of diverse isoprenoids.
Journal ArticleDOI
The studies on the limonene oxidation over the microporous TS-1 catalyst
TL;DR: The most beneficial conditions for the obtaining of high selectivity of 1,2-epoxylimonene, at simultaneously high values of the conversion of reactants and the efficiency of hydrogen peroxide, are as follows: the temperature 80°C, the molar ratio of limonene/H2O2 = 1:1, the methanol concentration 80 ¼, the TS-1 content 3 ¼ and the reaction time 10 days as mentioned in this paper.
Journal ArticleDOI
Synthesis and techno-economic assessment of microbial-based processes for terpenes production
TL;DR: The production of limonene appears to be the most reasonable short-term target because of its large market size and relatively easier to achieve break-even yield, and the key cost drivers to be feed glucose concentration, product yield and VVM.
Journal ArticleDOI
Rapid Discovery and Functional Characterization of Terpene Synthases from Four Endophytic Xylariaceae.
Weihua Wu,William T. Tran,Craig A. Taatjes,Jorge Alonso-Gutierrez,Jorge Alonso-Gutierrez,Taek Soon Lee,Taek Soon Lee,John M. Gladden,John M. Gladden +8 more
TL;DR: This study rapidly discovered and characterized 26 terpene synthases (TPSs) derived from four endophytic fungi known to produce mycodiesel hydrocarbons, and found that 12 TPS’s were functional, with the majority of them exhibiting both monoterpene and sesquiter pene synthase activity.
Journal ArticleDOI
Expanding the Isoprenoid Building Block Repertoire with an IPP Methyltransferase from Streptomyces monomycini.
Laura Drummond,Laura Drummond,Max J Kschowak,Max J Kschowak,Jürgen Breitenbach,Hendrik Wolff,Yi-Ming Shi,Jens Schrader,Helge B. Bode,Gerhard Sandmann,Markus Buchhaupt +10 more
TL;DR: In this article, a SAM-dependent methyltransferase was proposed to convert IPP into a variety of C6 and C7 prenyl pyrophosphates, which enabled the expansion of the terpenoid structure space beyond the borders predetermined by the isoprene rule.
References
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Journal ArticleDOI
Studies on transformation of Escherichia coli with plasmids
Douglas Hanahan,Douglas Hanahan +1 more
TL;DR: Competition with both transforming and non-transforming plasmids indicates that each cell is capable of taking up many DNA molecules, and that the establishment of a transformation event is neither helped nor hindered significantly by the presence of multiple plasmid molecules.
Journal ArticleDOI
Skyline: an open source document editor for creating and analyzing targeted proteomics experiments
Brendan MacLean,Daniela M. Tomazela,Nicholas J. Shulman,Matthew C. Chambers,Gregory L. Finney,Barbara Frewen,Randall Kern,David L. Tabb,Daniel C. Liebler,Michael J. MacCoss +9 more
TL;DR: The Skyline user interface simplifies the development of mass spectrometer methods and the analysis of data from targeted proteomics experiments performed using selected reaction monitoring (SRM).
Journal ArticleDOI
Production of the antimalarial drug precursor artemisinic acid in engineered yeast
Dae-Kyun Ro,Eric M. Paradise,Mario Ouellet,Karl Fisher,Karyn L. Newman,John M. Ndungu,Ho Kimberly,Eachus Rachel,Timothy S. Ham,James Kirby,Michelle C. Y. Chang,Sydnor T. Withers,Yoichiro Shiba,Richmond Sarpong,Jay D. Keasling +14 more
TL;DR: The engineering of Saccharomyces cerevisiae to produce high titres (up to 100 mg l-1) of artemisinic acid using an engineered mevalonate pathway, amorphadiene synthase, and a novel cytochrome P450 monooxygenase from A. annua that performs a three-step oxidation of amorpha-4,11-diene to art Artemisinic acid.
Journal ArticleDOI
Analysis of gene control signals by DNA fusion and cloning in Escherichia coli
TL;DR: Plasmid cloning vectors that enable insertion of DNA fragments between the inducible ara (arabinose) promoter and the lac (lactose) structural genes have been constructed and used for the detection and analysis of signals that control gene transcription.
Journal ArticleDOI
Engineering a mevalonate pathway in Escherichia coli for production of terpenoids
TL;DR: The strains developed in this study can serve as platform hosts for the production of any terpenoid compound for which a terpene synthase gene is available, and are the universal precursors to all isoprenoids.
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