Journal ArticleDOI
Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production
Jorge Alonso-Gutierrez,Rossana Chan,Rossana Chan,Tanveer S. Batth,Tanveer S. Batth,Paul D. Adams,Paul D. Adams,Jay D. Keasling,Christopher J. Petzold,Christopher J. Petzold,Taek Soon Lee,Taek Soon Lee +11 more
TLDR
A strain containing all mevalonate pathway genes in a single plasmid produced limonene at titers over 400mg/L from glucose, substantially higher than has been achieved in the past.About:
This article is published in Metabolic Engineering.The article was published on 2013-09-01. It has received 328 citations till now. The article focuses on the topics: Perillyl alcohol & Limonene.read more
Citations
More filters
Journal ArticleDOI
Distributing a metabolic pathway among a microbial consortium enhances production of natural products
TL;DR: Stable co-culture in the same bioreactor was achieved by designing a mutualistic relationship between the two species in which a metabolic intermediate produced by E. coli was used and functionalized by yeast.
Journal ArticleDOI
Fuelling the future: microbial engineering for the production of sustainable biofuels
TL;DR: This Review discusses how microorganisms can be explored for the production of next-generation biofuels, based on the ability of bacteria and fungi to use lignocellulose; through direct CO2 conversion by microalgae; using lithoautotrophs driven by solar electricity; or through the capacity of microorganisms to use methane generated from landfill.
Journal ArticleDOI
Escherichia coli as a host for metabolic engineering.
TL;DR: The physiological attributes of E. coli that are most relevant for metabolic engineering are discussed, as well as emerging techniques that enable efficient phenotype construction that address some of the future challenges in broadening substrate range and fighting phage infection.
Journal ArticleDOI
Engineering Limonene and Bisabolene Production in Wild Type and a Glycogen-Deficient Mutant of Synechococcus sp. PCC 7002.
TL;DR: The fast-growing unicellular euryhaline cyanobacterium Synechococcus sp.
Journal ArticleDOI
Microbial Synthesis of Pinene
TL;DR: It is demonstrated that the GPP concentration available to PS for cyclization alters the pinene isomer ratio, and it is speculated that pinene toxicity was limiting production; however, toxicity should not be limiting at current titers.
References
More filters
Journal ArticleDOI
Plasmid‐encoded protein: The principal factor in the “metabolic burden” associated with recombinant bacteria
TL;DR: Plasmid content results indicate that the plasmid copy number monotonically increases with decreasing growth rate, and the reduction in growth rate brought about by the expression of chloramphenicol‐acetyl‐transferase (CAT) and β‐lactamase is experimentally quantified.
Journal ArticleDOI
Construction of lycopene-overproducing E. coli strains by combining systematic and combinatorial gene knockout targets.
TL;DR: It is shown that systematic and combinatorial search methods for gene knockout targets that increase lycopene biosynthesis in strains of Escherichia coli yield two distinct gene sets, which affect product synthesis either through an increase in precursor availability or through (largely unknown) kinetic or regulatory mechanisms, respectively.
Journal ArticleDOI
Balancing a heterologous mevalonate pathway for improved isoprenoid production in Escherichia coli.
TL;DR: The results demonstrate that balancing carbon flux through the heterologous pathway is a key determinant in optimizing isoprenoid biosynthesis in microbial hosts and eliminated the pathway bottleneck and increased mevalonate production.
Journal ArticleDOI
Engineering microbial biofuel tolerance and export using efflux pumps
Mary J. Dunlop,Zain Y. Dossani,Zain Y. Dossani,Heather L. Szmidt,Heather L. Szmidt,Hou Cheng Chu,Hou Cheng Chu,Taek Soon Lee,Taek Soon Lee,Jay D. Keasling,Jay D. Keasling,Jay D. Keasling,Masood Z. Hadi,Masood Z. Hadi,Aindrila Mukhopadhyay,Aindrila Mukhopadhyay +15 more
TL;DR: This work used bioinformatics to generate a list of all efflux pumps from sequenced bacterial genomes and prioritized a subset of targets for cloning, and efficiently distinguished pumps that improved survival and identified pumps that restored growth in the presence of biofuel.
Journal ArticleDOI
BglBricks: A flexible standard for biological part assembly
JChristopher Anderson,John E. Dueber,Mariana Leguia,Gabriel C. Wu,Jonathan A. Goler,Jonathan A. Goler,Adam P. Arkin,Jay D. Keasling +7 more
TL;DR: The BglBrick standard provides a new, more flexible platform from which to generate standard biological parts and automate DNA assembly, and is demonstrated in three distinct applications, including the construction of constitutively active gene expression devices with a wide range of expression profiles.
Related Papers (5)
Engineering microbial biofuel tolerance and export using efflux pumps
High-level semi-synthetic production of the potent antimalarial artemisinin
Christopher J. Paddon,Patrick J. Westfall,Douglas J. Pitera,Kirsten R. Benjamin,Karl Fisher,Derek McPhee,Michael D. Leavell,Anna Tai,A. Main,Diana Eng,Devin R Polichuk,Keat H. Teoh,Darwin W. Reed,T. Treynor,Jacob R. Lenihan,Hanxiao Jiang,M. Fleck,S. Bajad,G. Dang,D. Dengrove,Don Diola,G. Dorin,Kenneth W. Ellens,Scott Fickes,J. Galazzo,Sara P. Gaucher,T. Geistlinger,Ronald Henry,M. Hepp,Tizita Horning,T. Iqbal,L. Kizer,B. Lieu,D. Melis,Nathan A. Moss,Rika Regentin,S. Secrest,H. Tsuruta,R. Vazquez,Lars F. Westblade,Lan Xu,M. Yu,Yansheng Zhang,Lishan Zhao,Jefferson C. Lievense,Patrick S. Covello,Jay D. Keasling,K. K. Reiling,N. S. Renninger,Jack D. Newman +49 more