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Journal ArticleDOI

Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production

Jorge Alonso-Gutierrez, +11 more
- 01 Sep 2013 - 
- Vol. 19, pp 33-41
TLDR
A strain containing all mevalonate pathway genes in a single plasmid produced limonene at titers over 400mg/L from glucose, substantially higher than has been achieved in the past.
About: 
This article is published in Metabolic Engineering.The article was published on 2013-09-01. It has received 328 citations till now. The article focuses on the topics: Perillyl alcohol & Limonene.

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Citations
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Journal ArticleDOI

Engineering improved bio-jet fuel tolerance in Escherichia coli using a transgenic library from the hydrocarbon-degrader Marinobacter aquaeolei

Timothy A. Tomko, +1 more
- 07 Oct 2015 - 
TL;DR: The genomes of hydrocarbon-tolerant microbes represent a rich resource for tolerance engineering and a single gene is identified that improves E. coli’s tolerance to the bio-jet fuel precursor pinene.
Journal ArticleDOI

Exploring novel bacterial terpene synthases.

Gajendar Komati Reddy, +7 more
- 30 Apr 2020 - 
TL;DR: It is demonstrated that terpene synthases with substrate promiscuity are widely distributed in nature, forming a rich resource for engineering terPene biosynthetic pathways for biotechnology.
Journal ArticleDOI

Alternative metabolic pathways and strategies to high-titre terpenoid production in<i>Escherichia coli</i>

- 01 Jan 2022 - 
TL;DR: In this paper , a review of strategies to increase terpenoid titres in Escherichia coli with a focus on alternative metabolic pathways is presented, which can lead to improved titres by providing higher orthogonality to native metabolism that redirects carbon flux, by avoiding toxic intermediates, by bypassing highlyregulated or bottleneck steps, or by being shorter and thus more efficient and easier to manipulate.
Journal ArticleDOI

Functional characterization of a geraniol synthase-encoding gene from Camptotheca acuminata and its application in production of geraniol in Escherichia coli

Fei Chen, +6 more
- 27 Jun 2016 - 
TL;DR: The functional characterization of CaGES from Camptotheca acuminata is reported, and its application in production of geraniol in Escherichia coli will be helpful to uncover the biosynthetic enigma of camptothecin and tCaGES will be an alternative to selectively produce geranio in E. coli with other metabolic engineering approaches.
Journal ArticleDOI

Heterologous expression of 2-methylisoborneol / 2 methylenebornane biosynthesis genes in Escherichia coli yields novel C11-terpenes.

Max J Kschowak, +5 more
- 19 Apr 2018 - 
TL;DR: A broad spectrum of novel terpenoids with eleven carbon atoms as byproducts of bacterial 2- methylisoborneol or 2-methylenebornane synthases is discovered and an extended mechanism for C11-terpene synthases are provided.
References
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Journal ArticleDOI

Studies on transformation of Escherichia coli with plasmids

Douglas Hanahan, +1 more
- 05 Jun 1983 - 
TL;DR: Competition with both transforming and non-transforming plasmids indicates that each cell is capable of taking up many DNA molecules, and that the establishment of a transformation event is neither helped nor hindered significantly by the presence of multiple plasmid molecules.
Journal ArticleDOI

Skyline: an open source document editor for creating and analyzing targeted proteomics experiments

Brendan MacLean, +9 more
- 01 Apr 2010 - 
TL;DR: The Skyline user interface simplifies the development of mass spectrometer methods and the analysis of data from targeted proteomics experiments performed using selected reaction monitoring (SRM).
Journal ArticleDOI

Production of the antimalarial drug precursor artemisinic acid in engineered yeast

Dae-Kyun Ro, +14 more
- 13 Apr 2006 - 
TL;DR: The engineering of Saccharomyces cerevisiae to produce high titres (up to 100 mg l-1) of artemisinic acid using an engineered mevalonate pathway, amorphadiene synthase, and a novel cytochrome P450 monooxygenase from A. annua that performs a three-step oxidation of amorpha-4,11-diene to art Artemisinic acid.
Journal ArticleDOI

Analysis of gene control signals by DNA fusion and cloning in Escherichia coli

Malcolm J. Casadaban, +1 more
- 05 Apr 1980 - 
TL;DR: Plasmid cloning vectors that enable insertion of DNA fragments between the inducible ara (arabinose) promoter and the lac (lactose) structural genes have been constructed and used for the detection and analysis of signals that control gene transcription.
Journal ArticleDOI

Engineering a mevalonate pathway in Escherichia coli for production of terpenoids

Vincent J. J. Martin, +4 more
- 01 Jul 2003 - 
TL;DR: The strains developed in this study can serve as platform hosts for the production of any terpenoid compound for which a terpene synthase gene is available, and are the universal precursors to all isoprenoids.
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Trending Questions (1)
What is use the of metabolic engineering? limonene?

Metabolic engineering is used to produce limonene, a valuable monoterpene used in the production of commodity chemicals and medicinal compounds.

See answers from 4 other papers