Journal ArticleDOI
Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production
Jorge Alonso-Gutierrez,Rossana Chan,Rossana Chan,Tanveer S. Batth,Tanveer S. Batth,Paul D. Adams,Paul D. Adams,Jay D. Keasling,Christopher J. Petzold,Christopher J. Petzold,Taek Soon Lee,Taek Soon Lee +11 more
TLDR
A strain containing all mevalonate pathway genes in a single plasmid produced limonene at titers over 400mg/L from glucose, substantially higher than has been achieved in the past.About:
This article is published in Metabolic Engineering.The article was published on 2013-09-01. It has received 328 citations till now. The article focuses on the topics: Perillyl alcohol & Limonene.read more
Citations
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Journal ArticleDOI
Engineering cyanobacteria for the production of a cyclic hydrocarbon fuel from CO2 and H2O
TL;DR: It is demonstrated that increasing the light intensity and metabolic flux improves limonene productivity in the engineered cyanobacterium, and envision that the method of using N2-fixing cyanobacteria as a cellular factory and CO2 and N2 as sustainable feedstock can be applied for the production of a wide range of commodity chemicals and drop-in-fuels.
Journal ArticleDOI
Engineering the productivity of recombinant Escherichia coli for limonene formation from glycerol in minimal media
Christian Willrodt,Christian David,Sjef Cornelissen,Bruno Bühler,Mattijs K. Julsing,Andreas Schmid +5 more
TL;DR: The two‐liquid phase fed‐batch setup led to the highest monoterpene concentration obtained with a recombinant microbial biocatalyst to date, and the growth‐dependent production of (S)‐limonene was shown for the first time in minimal media.
Journal ArticleDOI
Identifying and engineering the ideal microbial terpenoid production host.
Sandra Moser,Harald Pichler +1 more
TL;DR: An overview on employing recombinant bacteria and yeasts for heterologous de novo production of terpenoids is provided and Rationalizing the current stages of development for different terpenoid production hosts as well as future prospects shall provide a valuable decision basis for the selection and engineering of the cell factory(ies) for industrial production of Terpenoid target molecules.
Journal ArticleDOI
Microbial production of scent and flavor compounds.
TL;DR: Approaches and challenges for the production of compounds such as esters, terpenoids, aldehydes, and methyl ketones from microbial hosts are discussed in this review.
Journal ArticleDOI
Metabolic engineering of the pentose phosphate pathway for enhanced limonene production in the cyanobacterium Synechocysti s sp. PCC 6803
TL;DR: This study presents a feasible strategy to engineer cyanobacteria for photosynthetic production of isoprenoids, and engineered the model cyanobacterium Synechocystis sp.
References
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Journal ArticleDOI
Studies on transformation of Escherichia coli with plasmids
Douglas Hanahan,Douglas Hanahan +1 more
TL;DR: Competition with both transforming and non-transforming plasmids indicates that each cell is capable of taking up many DNA molecules, and that the establishment of a transformation event is neither helped nor hindered significantly by the presence of multiple plasmid molecules.
Journal ArticleDOI
Skyline: an open source document editor for creating and analyzing targeted proteomics experiments
Brendan MacLean,Daniela M. Tomazela,Nicholas J. Shulman,Matthew C. Chambers,Gregory L. Finney,Barbara Frewen,Randall Kern,David L. Tabb,Daniel C. Liebler,Michael J. MacCoss +9 more
TL;DR: The Skyline user interface simplifies the development of mass spectrometer methods and the analysis of data from targeted proteomics experiments performed using selected reaction monitoring (SRM).
Journal ArticleDOI
Production of the antimalarial drug precursor artemisinic acid in engineered yeast
Dae-Kyun Ro,Eric M. Paradise,Mario Ouellet,Karl Fisher,Karyn L. Newman,John M. Ndungu,Ho Kimberly,Eachus Rachel,Timothy S. Ham,James Kirby,Michelle C. Y. Chang,Sydnor T. Withers,Yoichiro Shiba,Richmond Sarpong,Jay D. Keasling +14 more
TL;DR: The engineering of Saccharomyces cerevisiae to produce high titres (up to 100 mg l-1) of artemisinic acid using an engineered mevalonate pathway, amorphadiene synthase, and a novel cytochrome P450 monooxygenase from A. annua that performs a three-step oxidation of amorpha-4,11-diene to art Artemisinic acid.
Journal ArticleDOI
Analysis of gene control signals by DNA fusion and cloning in Escherichia coli
TL;DR: Plasmid cloning vectors that enable insertion of DNA fragments between the inducible ara (arabinose) promoter and the lac (lactose) structural genes have been constructed and used for the detection and analysis of signals that control gene transcription.
Journal ArticleDOI
Engineering a mevalonate pathway in Escherichia coli for production of terpenoids
TL;DR: The strains developed in this study can serve as platform hosts for the production of any terpenoid compound for which a terpene synthase gene is available, and are the universal precursors to all isoprenoids.
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