Journal ArticleDOI
Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production
Jorge Alonso-Gutierrez,Rossana Chan,Rossana Chan,Tanveer S. Batth,Tanveer S. Batth,Paul D. Adams,Paul D. Adams,Jay D. Keasling,Christopher J. Petzold,Christopher J. Petzold,Taek Soon Lee,Taek Soon Lee +11 more
TLDR
A strain containing all mevalonate pathway genes in a single plasmid produced limonene at titers over 400mg/L from glucose, substantially higher than has been achieved in the past.About:
This article is published in Metabolic Engineering.The article was published on 2013-09-01. It has received 328 citations till now. The article focuses on the topics: Perillyl alcohol & Limonene.read more
Citations
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Journal ArticleDOI
Elevating Limonene Production in Oleaginous Yeast Yarrowia lipolytica via Genetic Engineering of Limonene Biosynthesis Pathway and Optimization of Medium Composition
TL;DR: The engineered strain was found to produce 165.3 mg/L limonene, which corresponds to the highest yield till date for the production ofLimonene in Y. lipolytica, and amongst all the carbon sources tested, the highest level of limonenes production was obtained from glycerol, and citrate was selected as an auxiliary carbon source.
Journal ArticleDOI
Investigation of biofuels from microorganism metabolism for use as anti-knock additives
TL;DR: In this paper, the anti-knock properties of bio-fuels that can be produced from microorganism metabolic processes were investigated using a single-cylinder Cooperative Fuel Research (CFR) engine.
Journal ArticleDOI
Metabolic engineering of Escherichia coli for production of mixed isoprenoid alcohols and their derivatives.
Bakht Zada,Chonglong Wang,Ji-Bin Park,Seong-Hee Jeong,Ju-Eon Park,Hawaibam Birla Singh,Seon-Won Kim +6 more
TL;DR: The promiscuous activity of NudB was newly identified and successfully used for production of isoprenoid-based alcohol mixtures, which are suitable as next-generation biofuels or commodity chemicals.
Journal ArticleDOI
Techno-economic assessment of microbial limonene production
TL;DR: A novel microbial limonene production process at industrial plant scale is designed by a new combined gas-stripping/solvent-scrubbing method and uncertainties of parameters used in TEA were addressed by sensitivity analysis.
Journal ArticleDOI
Isolation and characterization of novel mutations in the pSC101 origin that increase copy number
Mitchell G. Thompson,Nima Sedaghatian,Nima Sedaghatian,Jesus F. Barajas,Maren Wehrs,Maren Wehrs,Constance B. Bailey,Constance B. Bailey,Nurgul Kaplan,Nurgul Kaplan,Nathan J. Hillson,Nathan J. Hillson,Aindrila Mukhopadhyay,Aindrila Mukhopadhyay,Jay D. Keasling +14 more
TL;DR: Multiple novel mutations that increase the copy number of plasmids with the pSC101 origin retained compatibility with p15a, pBBR, and ColE1 origins of replication and may be useful in future metabolic engineering efforts that require medium or high-copy vectors compatible with p 15a- and Col E1-based plasids.
References
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Journal ArticleDOI
Studies on transformation of Escherichia coli with plasmids
Douglas Hanahan,Douglas Hanahan +1 more
TL;DR: Competition with both transforming and non-transforming plasmids indicates that each cell is capable of taking up many DNA molecules, and that the establishment of a transformation event is neither helped nor hindered significantly by the presence of multiple plasmid molecules.
Journal ArticleDOI
Skyline: an open source document editor for creating and analyzing targeted proteomics experiments
Brendan MacLean,Daniela M. Tomazela,Nicholas J. Shulman,Matthew C. Chambers,Gregory L. Finney,Barbara Frewen,Randall Kern,David L. Tabb,Daniel C. Liebler,Michael J. MacCoss +9 more
TL;DR: The Skyline user interface simplifies the development of mass spectrometer methods and the analysis of data from targeted proteomics experiments performed using selected reaction monitoring (SRM).
Journal ArticleDOI
Production of the antimalarial drug precursor artemisinic acid in engineered yeast
Dae-Kyun Ro,Eric M. Paradise,Mario Ouellet,Karl Fisher,Karyn L. Newman,John M. Ndungu,Ho Kimberly,Eachus Rachel,Timothy S. Ham,James Kirby,Michelle C. Y. Chang,Sydnor T. Withers,Yoichiro Shiba,Richmond Sarpong,Jay D. Keasling +14 more
TL;DR: The engineering of Saccharomyces cerevisiae to produce high titres (up to 100 mg l-1) of artemisinic acid using an engineered mevalonate pathway, amorphadiene synthase, and a novel cytochrome P450 monooxygenase from A. annua that performs a three-step oxidation of amorpha-4,11-diene to art Artemisinic acid.
Journal ArticleDOI
Analysis of gene control signals by DNA fusion and cloning in Escherichia coli
TL;DR: Plasmid cloning vectors that enable insertion of DNA fragments between the inducible ara (arabinose) promoter and the lac (lactose) structural genes have been constructed and used for the detection and analysis of signals that control gene transcription.
Journal ArticleDOI
Engineering a mevalonate pathway in Escherichia coli for production of terpenoids
TL;DR: The strains developed in this study can serve as platform hosts for the production of any terpenoid compound for which a terpene synthase gene is available, and are the universal precursors to all isoprenoids.
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