Journal ArticleDOI
Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production
Jorge Alonso-Gutierrez,Rossana Chan,Rossana Chan,Tanveer S. Batth,Tanveer S. Batth,Paul D. Adams,Paul D. Adams,Jay D. Keasling,Christopher J. Petzold,Christopher J. Petzold,Taek Soon Lee,Taek Soon Lee +11 more
TLDR
A strain containing all mevalonate pathway genes in a single plasmid produced limonene at titers over 400mg/L from glucose, substantially higher than has been achieved in the past.About:
This article is published in Metabolic Engineering.The article was published on 2013-09-01. It has received 328 citations till now. The article focuses on the topics: Perillyl alcohol & Limonene.read more
Citations
More filters
Journal ArticleDOI
A Growth-Based, High-Throughput Selection Platform Enables Remodeling of 4-Hydroxybenzoate Hydroxylase Active Site.
TL;DR: An aerobic, growth-based selection platform founded on NADP(H) redox balance restoration in Escherichia coli is reported and its application in the high-throughput evolution of an oxygenase is demonstrated.
Journal ArticleDOI
Optogenetic Control of Heterologous Metabolism in E. coli
TL;DR: This work engineering a version of the T7 RNA polymerase that drives the transition between biomass and product formation upon stimulation with red light is a significant advance for the field and greatly expands on previous work by other groups that have used optogenetic circuits to control heterologous metabolism in prokaryotic hosts.
Book ChapterDOI
Industrial Biotechnology: Escherichia coli as a Host
Matthew K. Theisen,James C. Liao +1 more
Journal ArticleDOI
Rationally optimized generation of integrated Escherichia coli with stable and high yield lycopene biosynthesis from heterologous mevalonate (MVA) and lycopene expression pathways.
Muhammad Hammad Hussain,Hong Qi,Waqas Qamar Zaman,Ali Mohsin,Yanlong Wei,Ning Zhang,Hongqing Fang,Zejian Wang,Haifeng Hang,Yingping Zhuang,Meijin Guo +10 more
TL;DR: In this paper, an integrated Escherichia coli system has been generated successfully, which resulted into stable and high lycopene production, and two modules of mevalonate (MVA) pathway and one module of Lycopene expression pathway were completely integrated in the chromosome.
Journal ArticleDOI
Regioselective Acetylation of C21 Hydroxysteroids by the Bacterial Chloramphenicol Acetyltransferase I
TL;DR: A new and efficient Escherichia‐coli‐based biocatalyst for the regioselective acetylation of C21 hydroxy groups in steroids of pharmaceutical interest is designed.
References
More filters
Journal ArticleDOI
Studies on transformation of Escherichia coli with plasmids
Douglas Hanahan,Douglas Hanahan +1 more
TL;DR: Competition with both transforming and non-transforming plasmids indicates that each cell is capable of taking up many DNA molecules, and that the establishment of a transformation event is neither helped nor hindered significantly by the presence of multiple plasmid molecules.
Journal ArticleDOI
Skyline: an open source document editor for creating and analyzing targeted proteomics experiments
Brendan MacLean,Daniela M. Tomazela,Nicholas J. Shulman,Matthew C. Chambers,Gregory L. Finney,Barbara Frewen,Randall Kern,David L. Tabb,Daniel C. Liebler,Michael J. MacCoss +9 more
TL;DR: The Skyline user interface simplifies the development of mass spectrometer methods and the analysis of data from targeted proteomics experiments performed using selected reaction monitoring (SRM).
Journal ArticleDOI
Production of the antimalarial drug precursor artemisinic acid in engineered yeast
Dae-Kyun Ro,Eric M. Paradise,Mario Ouellet,Karl Fisher,Karyn L. Newman,John M. Ndungu,Ho Kimberly,Eachus Rachel,Timothy S. Ham,James Kirby,Michelle C. Y. Chang,Sydnor T. Withers,Yoichiro Shiba,Richmond Sarpong,Jay D. Keasling +14 more
TL;DR: The engineering of Saccharomyces cerevisiae to produce high titres (up to 100 mg l-1) of artemisinic acid using an engineered mevalonate pathway, amorphadiene synthase, and a novel cytochrome P450 monooxygenase from A. annua that performs a three-step oxidation of amorpha-4,11-diene to art Artemisinic acid.
Journal ArticleDOI
Analysis of gene control signals by DNA fusion and cloning in Escherichia coli
TL;DR: Plasmid cloning vectors that enable insertion of DNA fragments between the inducible ara (arabinose) promoter and the lac (lactose) structural genes have been constructed and used for the detection and analysis of signals that control gene transcription.
Journal ArticleDOI
Engineering a mevalonate pathway in Escherichia coli for production of terpenoids
TL;DR: The strains developed in this study can serve as platform hosts for the production of any terpenoid compound for which a terpene synthase gene is available, and are the universal precursors to all isoprenoids.
Related Papers (5)
Engineering microbial biofuel tolerance and export using efflux pumps
High-level semi-synthetic production of the potent antimalarial artemisinin
Christopher J. Paddon,Patrick J. Westfall,Douglas J. Pitera,Kirsten R. Benjamin,Karl Fisher,Derek McPhee,Michael D. Leavell,Anna Tai,A. Main,Diana Eng,Devin R Polichuk,Keat H. Teoh,Darwin W. Reed,T. Treynor,Jacob R. Lenihan,Hanxiao Jiang,M. Fleck,S. Bajad,G. Dang,D. Dengrove,Don Diola,G. Dorin,Kenneth W. Ellens,Scott Fickes,J. Galazzo,Sara P. Gaucher,T. Geistlinger,Ronald Henry,M. Hepp,Tizita Horning,T. Iqbal,L. Kizer,B. Lieu,D. Melis,Nathan A. Moss,Rika Regentin,S. Secrest,H. Tsuruta,R. Vazquez,Lars F. Westblade,Lan Xu,M. Yu,Yansheng Zhang,Lishan Zhao,Jefferson C. Lievense,Patrick S. Covello,Jay D. Keasling,K. K. Reiling,N. S. Renninger,Jack D. Newman +49 more