Journal ArticleDOI
Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production
Jorge Alonso-Gutierrez,Rossana Chan,Rossana Chan,Tanveer S. Batth,Tanveer S. Batth,Paul D. Adams,Paul D. Adams,Jay D. Keasling,Christopher J. Petzold,Christopher J. Petzold,Taek Soon Lee,Taek Soon Lee +11 more
TLDR
A strain containing all mevalonate pathway genes in a single plasmid produced limonene at titers over 400mg/L from glucose, substantially higher than has been achieved in the past.About:
This article is published in Metabolic Engineering.The article was published on 2013-09-01. It has received 328 citations till now. The article focuses on the topics: Perillyl alcohol & Limonene.read more
Citations
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Journal ArticleDOI
Precursor prioritization for p-cymene production through synergistic integration of biology and chemistry
Hsi–Hsin Lin,Daniel Mendez-Perez,Jimin Park,Xi Wang,Yan Cheng,Jiajie Huo,Aindrila Mukhopadhyay,Taek Soon Lee,Brent H. Shanks +8 more
TL;DR: In this article , the authors demonstrate p-cymene production through the integration of biosynthesis and heterogeneous catalysis and show how a preferred biologically derived precursor could be determined.
Journal ArticleDOI
Biosynthesis of cannabigerol and cannabigerolic acid: the gateways to further cannabinoid production
TL;DR: In this paper , the identification and repurposing of aromatic prenyltransferase (AtaPT), which when coupled with native enzymes of C. sativa can form an Escherichia coli production system for CBGA in cell lysates and CBG in whole cells.
Book ChapterDOI
Biofuel production from renewable feedstocks: Progress through metabolic engineering
TL;DR: A brief overview of the worldwide scenario of biofuel production and the advancements made in their synthesis using metabolic engineering is provided in this paper , where the availability of genomic sequences of microbial species and the development of genome-scale metabolic models have aided in the fast-track reconstruction of cellular metabolism, which can assist in metabolic engineering.
References
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Journal ArticleDOI
Studies on transformation of Escherichia coli with plasmids
Douglas Hanahan,Douglas Hanahan +1 more
TL;DR: Competition with both transforming and non-transforming plasmids indicates that each cell is capable of taking up many DNA molecules, and that the establishment of a transformation event is neither helped nor hindered significantly by the presence of multiple plasmid molecules.
Journal ArticleDOI
Skyline: an open source document editor for creating and analyzing targeted proteomics experiments
Brendan MacLean,Daniela M. Tomazela,Nicholas J. Shulman,Matthew C. Chambers,Gregory L. Finney,Barbara Frewen,Randall Kern,David L. Tabb,Daniel C. Liebler,Michael J. MacCoss +9 more
TL;DR: The Skyline user interface simplifies the development of mass spectrometer methods and the analysis of data from targeted proteomics experiments performed using selected reaction monitoring (SRM).
Journal ArticleDOI
Production of the antimalarial drug precursor artemisinic acid in engineered yeast
Dae-Kyun Ro,Eric M. Paradise,Mario Ouellet,Karl Fisher,Karyn L. Newman,John M. Ndungu,Ho Kimberly,Eachus Rachel,Timothy S. Ham,James Kirby,Michelle C. Y. Chang,Sydnor T. Withers,Yoichiro Shiba,Richmond Sarpong,Jay D. Keasling +14 more
TL;DR: The engineering of Saccharomyces cerevisiae to produce high titres (up to 100 mg l-1) of artemisinic acid using an engineered mevalonate pathway, amorphadiene synthase, and a novel cytochrome P450 monooxygenase from A. annua that performs a three-step oxidation of amorpha-4,11-diene to art Artemisinic acid.
Journal ArticleDOI
Analysis of gene control signals by DNA fusion and cloning in Escherichia coli
TL;DR: Plasmid cloning vectors that enable insertion of DNA fragments between the inducible ara (arabinose) promoter and the lac (lactose) structural genes have been constructed and used for the detection and analysis of signals that control gene transcription.
Journal ArticleDOI
Engineering a mevalonate pathway in Escherichia coli for production of terpenoids
TL;DR: The strains developed in this study can serve as platform hosts for the production of any terpenoid compound for which a terpene synthase gene is available, and are the universal precursors to all isoprenoids.
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