Journal ArticleDOI
Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production
Jorge Alonso-Gutierrez,Rossana Chan,Rossana Chan,Tanveer S. Batth,Tanveer S. Batth,Paul D. Adams,Paul D. Adams,Jay D. Keasling,Christopher J. Petzold,Christopher J. Petzold,Taek Soon Lee,Taek Soon Lee +11 more
TLDR
A strain containing all mevalonate pathway genes in a single plasmid produced limonene at titers over 400mg/L from glucose, substantially higher than has been achieved in the past.About:
This article is published in Metabolic Engineering.The article was published on 2013-09-01. It has received 328 citations till now. The article focuses on the topics: Perillyl alcohol & Limonene.read more
Citations
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Journal ArticleDOI
Distributing a metabolic pathway among a microbial consortium enhances production of natural products
TL;DR: Stable co-culture in the same bioreactor was achieved by designing a mutualistic relationship between the two species in which a metabolic intermediate produced by E. coli was used and functionalized by yeast.
Journal ArticleDOI
Fuelling the future: microbial engineering for the production of sustainable biofuels
TL;DR: This Review discusses how microorganisms can be explored for the production of next-generation biofuels, based on the ability of bacteria and fungi to use lignocellulose; through direct CO2 conversion by microalgae; using lithoautotrophs driven by solar electricity; or through the capacity of microorganisms to use methane generated from landfill.
Journal ArticleDOI
Escherichia coli as a host for metabolic engineering.
TL;DR: The physiological attributes of E. coli that are most relevant for metabolic engineering are discussed, as well as emerging techniques that enable efficient phenotype construction that address some of the future challenges in broadening substrate range and fighting phage infection.
Journal ArticleDOI
Engineering Limonene and Bisabolene Production in Wild Type and a Glycogen-Deficient Mutant of Synechococcus sp. PCC 7002.
TL;DR: The fast-growing unicellular euryhaline cyanobacterium Synechococcus sp.
Journal ArticleDOI
Microbial Synthesis of Pinene
TL;DR: It is demonstrated that the GPP concentration available to PS for cyclization alters the pinene isomer ratio, and it is speculated that pinene toxicity was limiting production; however, toxicity should not be limiting at current titers.
References
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Journal ArticleDOI
Bioconversion of limonene to increased concentrations of perillic acid by Pseudomonas putida GS1 in a fed-batch reactor
TL;DR: P. putida GS1 can use p-cymene as a single source of carbon and energy, and the enzymes that are responsible for the conversion of limonene to perillic acid belong to the degradation pathway of p- Cymene.
Patent
Jet fuel compositions
TL;DR: In this article, the authors proposed a low temperature operability of jet fuel compositions consisting of a jet fuel and at least one of the following additives: (i) a copolymer of ethylene and one unsaturated ester selected from: vinyl esters having at least 5 carbon atoms, alkyl (meth)acrylates, di-alkyl fumarates and dialkyl maleates.
Book ChapterDOI
Targeted Proteomics for Metabolic Pathway Optimization
TL;DR: A targeted proteomics approach that uses selected-reaction monitoring (SRM) mass spectrometry to quantify multiple proteins in a sample and provides high selectivity and high sensitivity to enable rapid quantification ofmultiple proteins in an engineered pathway regardless of sequence or organism of origin.
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