Journal ArticleDOI
Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production
Jorge Alonso-Gutierrez,Rossana Chan,Rossana Chan,Tanveer S. Batth,Tanveer S. Batth,Paul D. Adams,Paul D. Adams,Jay D. Keasling,Christopher J. Petzold,Christopher J. Petzold,Taek Soon Lee,Taek Soon Lee +11 more
TLDR
A strain containing all mevalonate pathway genes in a single plasmid produced limonene at titers over 400mg/L from glucose, substantially higher than has been achieved in the past.About:
This article is published in Metabolic Engineering.The article was published on 2013-09-01. It has received 328 citations till now. The article focuses on the topics: Perillyl alcohol & Limonene.read more
Citations
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ART: A machine learning Automated Recommendation Tool for synthetic biology
TL;DR: The Automated Recommendation Tool (ART) as discussed by the authors leverages machine learning and probabilistic modeling techniques to guide synthetic biology in a systematic fashion, without the need for a full mechanistic understanding of the biological system.
Journal ArticleDOI
The isoprenoid alcohol pathway, a synthetic route for isoprenoid biosynthesis.
TL;DR: The IPA pathway provides an alternative route to isoprenoids that is more energy efficient than native pathways and can serve as a platform for targeting a repertoire ofIsoprenoid compounds with application as high-value pharmaceuticals, commodity chemicals, and fuels.
MOESM1 of Metabolic engineering of oleaginous yeast Yarrowia lipolytica for limonene overproduction
TL;DR: This is the first report on limonene biosynthesis in oleaginous yeast Y. lipolytica by heterologous expression of codon-optimized tLS and tNDPS1 genes and it is demonstrated that Y.lipolyTica provides a compelling platform for the overproduction of limonenes derivatives, and even other monoterpenes.
Journal ArticleDOI
Techno-economic analysis and life-cycle greenhouse gas mitigation cost of five routes to bio-jet fuel blendstocks
Nawa Raj Baral,Olga Kavvada,Daniel Mendez-Perez,Aindrila Mukhopadhyay,Taek Soon Lee,Blake A. Simmons,Corinne D. Scown +6 more
TL;DR: In this article, the authors presented a detailed techno-economic analysis and sensitivity analysis, including estimated minimum selling price (MSP), and life-cycle greenhouse gas (GHG) mitigation costs for five routes to four potential bio-jet fuel molecules.
Journal Article
Preclinical development and clinical use of perillyl alcohol for chemoprevention and cancer therapy.
TL;DR: The vicissitudes of POH's evaluation as an anticancer agent, and its most recent success in therapy of patients with malignant brain tumors are presented.
References
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Journal ArticleDOI
Studies on transformation of Escherichia coli with plasmids
Douglas Hanahan,Douglas Hanahan +1 more
TL;DR: Competition with both transforming and non-transforming plasmids indicates that each cell is capable of taking up many DNA molecules, and that the establishment of a transformation event is neither helped nor hindered significantly by the presence of multiple plasmid molecules.
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Skyline: an open source document editor for creating and analyzing targeted proteomics experiments
Brendan MacLean,Daniela M. Tomazela,Nicholas J. Shulman,Matthew C. Chambers,Gregory L. Finney,Barbara Frewen,Randall Kern,David L. Tabb,Daniel C. Liebler,Michael J. MacCoss +9 more
TL;DR: The Skyline user interface simplifies the development of mass spectrometer methods and the analysis of data from targeted proteomics experiments performed using selected reaction monitoring (SRM).
Journal ArticleDOI
Production of the antimalarial drug precursor artemisinic acid in engineered yeast
Dae-Kyun Ro,Eric M. Paradise,Mario Ouellet,Karl Fisher,Karyn L. Newman,John M. Ndungu,Ho Kimberly,Eachus Rachel,Timothy S. Ham,James Kirby,Michelle C. Y. Chang,Sydnor T. Withers,Yoichiro Shiba,Richmond Sarpong,Jay D. Keasling +14 more
TL;DR: The engineering of Saccharomyces cerevisiae to produce high titres (up to 100 mg l-1) of artemisinic acid using an engineered mevalonate pathway, amorphadiene synthase, and a novel cytochrome P450 monooxygenase from A. annua that performs a three-step oxidation of amorpha-4,11-diene to art Artemisinic acid.
Journal ArticleDOI
Analysis of gene control signals by DNA fusion and cloning in Escherichia coli
TL;DR: Plasmid cloning vectors that enable insertion of DNA fragments between the inducible ara (arabinose) promoter and the lac (lactose) structural genes have been constructed and used for the detection and analysis of signals that control gene transcription.
Journal ArticleDOI
Engineering a mevalonate pathway in Escherichia coli for production of terpenoids
TL;DR: The strains developed in this study can serve as platform hosts for the production of any terpenoid compound for which a terpene synthase gene is available, and are the universal precursors to all isoprenoids.
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