Journal ArticleDOI
Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production
Jorge Alonso-Gutierrez,Rossana Chan,Rossana Chan,Tanveer S. Batth,Tanveer S. Batth,Paul D. Adams,Paul D. Adams,Jay D. Keasling,Christopher J. Petzold,Christopher J. Petzold,Taek Soon Lee,Taek Soon Lee +11 more
TLDR
A strain containing all mevalonate pathway genes in a single plasmid produced limonene at titers over 400mg/L from glucose, substantially higher than has been achieved in the past.About:
This article is published in Metabolic Engineering.The article was published on 2013-09-01. It has received 328 citations till now. The article focuses on the topics: Perillyl alcohol & Limonene.read more
Citations
More filters
Book ChapterDOI
Cell-Free Systems for Sustainable Production of Biofuels
TL;DR: In this paper , the authors discuss recent progress and uses of cell-free system for production of biofuels toward industrial applications, and discuss the use of cell free system for the production of various important bio-fuels.
Patent
Genetically engineered microorganisms and processes for the production of cannabinoids from a carbon source precursor
TL;DR: In this paper, a method for biosynthetic production of cannabinoids in microorganisms from a carbon source precursor is described, and a system for tuning the method to produce different cannabinoids of interest by systematically modulating the enzymes encoded by the genetic modifications introduced in the microorganism.
Patent
Improved heterotrophic production methods for microbial biomass and bioproducts
TL;DR: In this article, a method for synthesizing a product of interest by culturing a microalgal cell producing the product in the dark in a culture medium comprising an organic acid as a fixed carbon source, wherein the micro algal cell is a facultative heterotroph.
Review Article Preclinical development and clinical use of perillyl alcohol for chemoprevention and cancer therapy
TL;DR: The vicissitudes of POH's evaluation as an anticancer agent, and its most recent success in therapy of patients with malignant brain tumors are presented.
References
More filters
Journal ArticleDOI
Studies on transformation of Escherichia coli with plasmids
Douglas Hanahan,Douglas Hanahan +1 more
TL;DR: Competition with both transforming and non-transforming plasmids indicates that each cell is capable of taking up many DNA molecules, and that the establishment of a transformation event is neither helped nor hindered significantly by the presence of multiple plasmid molecules.
Journal ArticleDOI
Skyline: an open source document editor for creating and analyzing targeted proteomics experiments
Brendan MacLean,Daniela M. Tomazela,Nicholas J. Shulman,Matthew C. Chambers,Gregory L. Finney,Barbara Frewen,Randall Kern,David L. Tabb,Daniel C. Liebler,Michael J. MacCoss +9 more
TL;DR: The Skyline user interface simplifies the development of mass spectrometer methods and the analysis of data from targeted proteomics experiments performed using selected reaction monitoring (SRM).
Journal ArticleDOI
Production of the antimalarial drug precursor artemisinic acid in engineered yeast
Dae-Kyun Ro,Eric M. Paradise,Mario Ouellet,Karl Fisher,Karyn L. Newman,John M. Ndungu,Ho Kimberly,Eachus Rachel,Timothy S. Ham,James Kirby,Michelle C. Y. Chang,Sydnor T. Withers,Yoichiro Shiba,Richmond Sarpong,Jay D. Keasling +14 more
TL;DR: The engineering of Saccharomyces cerevisiae to produce high titres (up to 100 mg l-1) of artemisinic acid using an engineered mevalonate pathway, amorphadiene synthase, and a novel cytochrome P450 monooxygenase from A. annua that performs a three-step oxidation of amorpha-4,11-diene to art Artemisinic acid.
Journal ArticleDOI
Analysis of gene control signals by DNA fusion and cloning in Escherichia coli
TL;DR: Plasmid cloning vectors that enable insertion of DNA fragments between the inducible ara (arabinose) promoter and the lac (lactose) structural genes have been constructed and used for the detection and analysis of signals that control gene transcription.
Journal ArticleDOI
Engineering a mevalonate pathway in Escherichia coli for production of terpenoids
TL;DR: The strains developed in this study can serve as platform hosts for the production of any terpenoid compound for which a terpene synthase gene is available, and are the universal precursors to all isoprenoids.
Related Papers (5)
Engineering microbial biofuel tolerance and export using efflux pumps
High-level semi-synthetic production of the potent antimalarial artemisinin
Christopher J. Paddon,Patrick J. Westfall,Douglas J. Pitera,Kirsten R. Benjamin,Karl Fisher,Derek McPhee,Michael D. Leavell,Anna Tai,A. Main,Diana Eng,Devin R Polichuk,Keat H. Teoh,Darwin W. Reed,T. Treynor,Jacob R. Lenihan,Hanxiao Jiang,M. Fleck,S. Bajad,G. Dang,D. Dengrove,Don Diola,G. Dorin,Kenneth W. Ellens,Scott Fickes,J. Galazzo,Sara P. Gaucher,T. Geistlinger,Ronald Henry,M. Hepp,Tizita Horning,T. Iqbal,L. Kizer,B. Lieu,D. Melis,Nathan A. Moss,Rika Regentin,S. Secrest,H. Tsuruta,R. Vazquez,Lars F. Westblade,Lan Xu,M. Yu,Yansheng Zhang,Lishan Zhao,Jefferson C. Lievense,Patrick S. Covello,Jay D. Keasling,K. K. Reiling,N. S. Renninger,Jack D. Newman +49 more