Microglia modulate hippocampal synaptic transmission and sleep duration along the light/dark cycle
Giorgio Corsi,Katherine Picard,Maria Amalia Di Castro,Stefano Garofalo,Federico Tucci,Giuseppina Chece,Claudio Del Percio,M.T. Golia,Marcello Raspa,Ferdinando Scavizzi,Fanny Decoeur,Clotilde Lauro,Mara Rigamonti,Fabio Iannello,Davide Ragozzino,Eleonora Russo,Giovanni Bernardini,Agnès Nadjar,Marie-Ève Tremblay,Marie-Ève Tremblay,Marie-Ève Tremblay,Claudio Babiloni,Laura Maggi,Cristina Limatola +23 more
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TLDR
In this article, the effects of microglial depletion with the colony stimulating factor-1 receptor antagonist PLX5622 on the sleep/wake cycle and on hippocampal synaptic transmission in male mice were investigated.Abstract:
Microglia, the brain's resident macrophages, actively contributes to the homeostasis of cerebral parenchyma by sensing neuronal activity and supporting synaptic remodeling and plasticity. While several studies demonstrated different roles for astrocytes in sleep, the contribution of microglia in the regulation of sleep/wake cycle and in the modulation of synaptic activity in the different day phases has not been deeply investigated. Using light as a zeitgeber cue, we studied the effects of microglial depletion with the colony stimulating factor-1 receptor antagonist PLX5622 on the sleep/wake cycle and on hippocampal synaptic transmission in male mice. Our data demonstrate that almost complete microglial depletion increases the duration of NREM sleep and reduces the hippocampal excitatory neurotransmission. The fractalkine receptor CX3CR1 plays a relevant role in these effects, because cx3cr1GFP/GFP mice recapitulate what found in PLX5622-treated mice. Furthermore, during the light phase, microglia express lower levels of cx3cr1 and a reduction of cx3cr1 expression is also observed when cultured microglial cells are stimulated by ATP, a purinergic molecule released during sleep. Our findings suggest that microglia participate in the regulation of sleep, adapting their cx3cr1 expression in response to the light/dark phase, and modulating synaptic activity in a phase-dependent manner.read more
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Microglia maintain the normal structure and function of the hippocampal astrocyte network
TL;DR: The results reveal the importance of microglia in homeostatic regulation of the astrocyte syncytium and scaling of synaptic transmission and uncover a new direction for future studies interrogatingmicroglia function in various physiological and pathological contexts.
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Cortical diurnal rhythms remain intact with microglial depletion
TL;DR: In this article , microglia play an important role in the regulation of condensed extracellular matrix structures called perineuronal nets (PNNs), and it has been suggested that PNNs are also regulated in a circadian and diurnal manner.
Journal ArticleDOI
Cortical diurnal rhythms remain intact with microglial depletion
TL;DR: In this article , microglia play an important role in the regulation of condensed extracellular matrix structures called perineuronal nets (PNNs), and it has been suggested that PNNs are also regulated in a circadian and diurnal manner.
Journal ArticleDOI
Identification of hyper‐ramified microglia in the CA1 region of the mouse hippocampus potentially associated with stress resilience
Risako Fujikawa,Shozo Jinno +1 more
TL;DR: The results suggest that hyper‐ramified microglia in the hippocampus may be associated with stress resilience via the modulation of synaptic transmission.
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Sleep decreases neuronal activity control of microglial dynamics in mice
Ines Hristovska,Mariona Serras Robert,Kassandre Combet,J. Honnorat,Jean Christian Comte,Olivier Pascual +5 more
TL;DR: In this paper , the authors investigated neuron-microglia contacts and microglia morphodynamics in mice in an activity-dependent context such as the vigilance states, and they found that sleep modulates microglial morphodynamics through Cx3cr1 signaling.
References
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Journal ArticleDOI
Analyzing real-time PCR data by the comparative C(T) method.
TL;DR: This protocol provides an overview of the comparative CT method for quantitative gene expression studies and various examples to present quantitative gene Expression data using this method.
Journal ArticleDOI
Fate Mapping Analysis Reveals That Adult Microglia Derive from Primitive Macrophages
Florent Ginhoux,Florent Ginhoux,Melanie Greter,Marylene Leboeuf,Sayan Nandi,Peter See,Solen Gokhan,Mark F. Mehler,Simon J. Conway,Lai Guan Ng,E. Richard Stanley,Igor M. Samokhvalov,Miriam Merad +12 more
TL;DR: Results identify microglia as an ontogenically distinct population in the mononuclear phagocyte system and have implications for the use of embryonically derived microglial progenitors for the treatment of various brain disorders.
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Sleep Drives Metabolite Clearance From the Adult Brain
Lulu Xie,Hongyi Kang,Qiwu Xu,Michael Chen,Yonghong Liao,Meenakshisundaram Thiyagarajan,John O’Donnell,Daniel J. Christensen,Charles Nicholson,Jeffrey J. Iliff,Takahiro Takano,Rashid Deane +11 more
TL;DR: It is reported that sleep has a critical function in ensuring metabolic homeostasis and convective fluxes of interstitial fluid increased the rate of β-amyloid clearance during sleep, suggesting the restorative function of sleep may be a consequence of the enhanced removal of potentially neurotoxic waste products that accumulate in the awake central nervous system.
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Synaptic Pruning by Microglia Is Necessary for Normal Brain Development
Rosa C. Paolicelli,Giulia Bolasco,Francesca Pagani,Laura Maggi,Maria Scianni,Patrizia Panzanelli,Maurizio Giustetto,Tiago Ferreira,Eva Guiducci,Laura Dumas,Davide Ragozzino,Cornelius Gross +11 more
TL;DR: It is shown that microglia actively engulf synaptic material and play a major role in synaptic pruning during postnatal development in mice and this work suggests that deficits in microglian function may contribute to synaptic abnormalities seen in some neurodevelopmental disorders.
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Analysis of fractalkine receptor CX(3)CR1 function by targeted deletion and green fluorescent protein reporter gene insertion.
Steffen Jung,Julio Aliberti,Petra Graemmel,Mary Jean Sunshine,Georg W. Kreutzberg,Alan Sher,Dan R. Littman +6 more
TL;DR: Defying anticipated FKN functions, absence of CX3CR1 interferes neither with monocyte extravasation in a peritonitis model nor with DC migration and differentiation in response to microbial antigens or contact sensitizers.