Mitotic Exit in the Absence of Separase Activity
Ying Lu,Frederick R. Cross +1 more
TLDR
The first quantitative measure for Cdc14 release based on colocalization with the Net1 nucleolar anchor is defined, indicating efficient CDC14 release upon MEN activation; release driven by Esp1 in the absence of microtubules was inefficient and incapable of driving ME.Abstract:
In budding yeast, three interdigitated pathways regulate mitotic exit (ME): mitotic cyclin–cyclin-dependent kinase (Cdk) inactivation; the Cdc14 early anaphase release (FEAR) network, including a n...read more
Citations
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Dependence of Chs2 ER export on dephosphorylation by cytoplasmic Cdc14 ensures that septum formation follows mitosis.
TL;DR: The interdependence of chromosome segregation, MEN activation, decrease in mitotic CDK activity, and Cdc14 dispersal provides an effective mechanism for cells to order late mitotic events.
Journal ArticleDOI
Genetic instability in budding and fission yeast—sources and mechanisms
TL;DR: Various factors that may influence genome stability, such as cellular ploidy, the phase of the cell cycle, transcriptional activity of a particular region of DNA, the proficiency of DNA quality control systems, and finally potential exposure to endogenous or environmental stress are discussed.
Journal ArticleDOI
Licensing of Yeast Centrosome Duplication Requires Phosphoregulation of Sfi1
Jennifer S Avena,Shannon Burns,Zulin Yu,Christopher C. Ebmeier,William M. Old,Sue L. Jaspersen,Mark Winey +6 more
TL;DR: This study identifies Sfi1, a conserved component of centrosomes, as the first Cdk substrate required to restrict centrosome duplication to once per cell cycle and provides evidence that the protein phosphatase Cdc14 has the converse role of activating licensing, likely via dephosphorylation of Sfi2.
Journal ArticleDOI
Analysis of the mitotic exit control system using locked levels of stable mitotic cyclin.
TL;DR: Testing the inhibition of mitotic exit in budding yeast using graded levels of stable mitotic cyclin (Clb2) shows results inconsistent with the published ODE model, but revision of the model to allow Cdc14/Clb 2 balance to control mitoticExit corrects these discrepancies, providing theoretical support for the conclusions.
References
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Journal ArticleDOI
Comprehensive Identification of Cell Cycle–regulated Genes of the Yeast Saccharomyces cerevisiae by Microarray Hybridization
Paul T. Spellman,Gavin Sherlock,Gavin Sherlock,Michael Q. Zhang,Vishwanath R. Iyer,Kirk R. Anders,Michael B. Eisen,Patrick O. Brown,Patrick O. Brown,David Botstein,Bruce Futcher +10 more
TL;DR: A comprehensive catalog of yeast genes whose transcript levels vary periodically within the cell cycle is created, and it is found that the mRNA levels of more than half of these 800 genes respond to one or both of these cyclins.
Journal ArticleDOI
Cohesins: chromosomal proteins that prevent premature separation of sister chromatids
TL;DR: Three chromosmal proteins that prevent premature separation of sister chromatids in yeast are described, two of which are members of the SMC family, which are putative ATPases with coiled-coil domains.
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Sister-chromatid separation at anaphase onset is promoted by cleavage of the cohesin subunit Scc1
TL;DR: It is shown that Esp1 causes the dissociation of Scc1 from chromosomes by stimulating its cleavage by proteolysis, and a mutant SCC1 is described that is resistant to Esp1-dependent cleavage and which blocks both sister-chromatid separation and the dissociations from chromosomes.
Journal ArticleDOI
Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression
Journal ArticleDOI
The B-type cyclin kinase inhibitor p40SIC1 controls the G1 to S transition in S. cerevisiae
TL;DR: It is shown that DNA replication also requires activation of Cdc28 by B-type (Clb) cyclins, and proteolysis of a cyclin-specific inhibitor of CDC28 is an essential aspect of the G1 to S phase transition.
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