Journal ArticleDOI
Next-generation proteomics: towards an integrative view of proteome dynamics
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TLDR
MS-based proteomics is starting to mature and to deliver through a combination of developments in instrumentation, sample preparation and computational analysis, and to highlight recent applications.Abstract:
Next-generation sequencing allows the analysis of genomes, including those representing disease states. However, the causes of most disorders are multifactorial, and systems-level approaches, including the analysis of proteomes, are required for a more comprehensive understanding. The proteome is extremely multifaceted owing to splicing and protein modifications, and this is further amplified by the interconnectivity of proteins into complexes and signalling networks that are highly divergent in time and space. Proteome analysis heavily relies on mass spectrometry (MS). MS-based proteomics is starting to mature and to deliver through a combination of developments in instrumentation, sample preparation and computational analysis. Here we describe this emerging next generation of proteomics and highlight recent applications.read more
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Journal ArticleDOI
Towards automation in protein digestion: Development of a monolithic trypsin immobilized reactor for highly efficient on-line digestion and analysis
TL;DR: Novel trypsin-based monolithic immobilized enzyme reactors, obtained by covalent immobilization on convective interaction media (CIMac™) analytical columns, showed high sensitivity down to the pmole level and were suitable for the analysis of high-molecular weight proteins.
Book ChapterDOI
Next-Generation Sequencing.
TL;DR: Next-generation sequencing technologies provided the capability to rapidly sequence exomes, transcriptomes, and genomes at relatively low cost and revealed, for the first time, that serous and endometrioid endometrial cancers can be classified into four distinct molecular subgroups.
Journal ArticleDOI
Comparative proteomic analysis of the contractile-protein-depleted fraction from normal versus dystrophic skeletal muscle
TL;DR: To remove a large portion of highly abundant contractile proteins from skeletal muscle homogenates without the usage of major manipulative steps, differential centrifugation was used to decisively reduce the sample complexity of crude muscle tissue extracts.
Journal ArticleDOI
Discriminating Residue Substitutions in a Single Protein Molecule Using a Sub-nanopore
TL;DR: A comparison of the patterns in the current fluctuations associated with the two practically identical histones supported the conclusion that a sub-nanopore was sensitive enough to discriminate amino acid substitutions in the sequence of a single protein molecule by measuring volumes of 0.1 nm3 per read.
Journal ArticleDOI
Dual Matrix-Based Immobilized Trypsin for Complementary Proteolytic Digestion and Fast Proteomics Analysis with Higher Protein Sequence Coverage
Chao Fan,Zhaomei Shi,Zhaomei Shi,Yiting Pan,Zifeng Song,Wanjun Zhang,Xinyuan Zhao,Fang Tian,Bo Peng,Weijie Qin,Yun Cai,Xiaohong Qian +11 more
TL;DR: The dual matrix-based complementary digestion shows particular advantage in the digestion of membrane proteins, as twice the number of identified peptides is obtained compared with solution digestion using free proteases, demonstrating its potential as a promising alternative to promote proteomics analysis with higher protein sequence coverage.
References
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