Journal ArticleDOI
Next-generation proteomics: towards an integrative view of proteome dynamics
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TLDR
MS-based proteomics is starting to mature and to deliver through a combination of developments in instrumentation, sample preparation and computational analysis, and to highlight recent applications.Abstract:
Next-generation sequencing allows the analysis of genomes, including those representing disease states. However, the causes of most disorders are multifactorial, and systems-level approaches, including the analysis of proteomes, are required for a more comprehensive understanding. The proteome is extremely multifaceted owing to splicing and protein modifications, and this is further amplified by the interconnectivity of proteins into complexes and signalling networks that are highly divergent in time and space. Proteome analysis heavily relies on mass spectrometry (MS). MS-based proteomics is starting to mature and to deliver through a combination of developments in instrumentation, sample preparation and computational analysis. Here we describe this emerging next generation of proteomics and highlight recent applications.read more
Citations
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Intelligence Algorithms for Protein Classification by Mass Spectrometry.
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Impact of Amidination on Peptide Fragmentation and Identification in Shotgun Proteomics
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Three-dimensional ordered titanium dioxide-zirconium dioxide film-based microfluidic device for efficient on-chip phosphopeptide enrichment.
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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification.
Anthony A. High,Haiyan Tan,Vishwajeeth Pagala,Mingming Niu,Ji-Hoon Cho,Xusheng Wang,Bing Bai,Junmin Peng +7 more
TL;DR: A deep-proteomics profiling protocol that combines 10-plex tandem mass tag (TMT) labeling with an extensive LC/LC-MS/MS platform, and post-MS computational interference correction to accurately quantitate whole proteomes is introduced.
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