Journal ArticleDOI
Next-generation proteomics: towards an integrative view of proteome dynamics
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TLDR
MS-based proteomics is starting to mature and to deliver through a combination of developments in instrumentation, sample preparation and computational analysis, and to highlight recent applications.Abstract:
Next-generation sequencing allows the analysis of genomes, including those representing disease states. However, the causes of most disorders are multifactorial, and systems-level approaches, including the analysis of proteomes, are required for a more comprehensive understanding. The proteome is extremely multifaceted owing to splicing and protein modifications, and this is further amplified by the interconnectivity of proteins into complexes and signalling networks that are highly divergent in time and space. Proteome analysis heavily relies on mass spectrometry (MS). MS-based proteomics is starting to mature and to deliver through a combination of developments in instrumentation, sample preparation and computational analysis. Here we describe this emerging next generation of proteomics and highlight recent applications.read more
Citations
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Journal ArticleDOI
Proteomic tools to study drug function
TL;DR: This review discusses affinity pull-down approaches to study direct drug-target interaction, methods which use alterations in protein stability as a measure for drug binding and the biological relevance of PTM enrichments to study the effect of inhibitors on cellular signalling.
Preterm intraamniotic infection and inflammation: Search for protein biomarkers via proteomics approach
Niu Jin Tan,Amilia Afzan Mohd Jamil,Norhafizah Mohtarrudin,Sunhare Raksha,Thilakavathy Karrupiah +4 more
TL;DR: A panel of highly sensitive and specific protein biomarkers that are able to discriminate IAI pregnant women at high risk of PTD with PLIM from PPROM are urgently needed to improve the treatment benefits.
Journal ArticleDOI
Proteomics Study on the Cerebrospinal Fluid of Patients with Encephalitis.
Liu-Lin Xiong,Lu-Lu Xue,Yan-Jun Chen,Ruo-Lan Du,Qian Wang,Song Wen,Lin Zhou,Tao Liu,Ting-Hua Wang,Changyin Yu +9 more
TL;DR: In this article, label-free quantitative proteomics was applied to analyze differentially expressed proteins (DEPs) in the cerebrospinal fluid (CSF) of patients with encephalitis.
Bioinformatic identification of disease driver networks using functional profiling data
TL;DR: This document summarizes current capabilities, research and operational priorities, and plans for further studies that were established at the 2015 USGS workshop on quantitative hazard assessments of earthquake-triggered landsliding and liquefaction in the Central American region.
Book ChapterDOI
The Genus Corynebacterium in the Genomic Era
Carlos Leonardo Araújo,Jorianne Thyeska Castro Alves,Alyne Cristina Sodré Lima,Larissa M. Dias,Patrícia Garcia Ernando da Silva,Joana Montezano Marques,Vasco Azevedo,Artur Silva,Adriana Ribeiro Carneiro Folador +8 more
TL;DR: This chapter will address the main omics approaches used in studies involving the genus Corynebacterium, Gram-positive microorganisms that can be isolated from many diverse environments.
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