Journal ArticleDOI
Next-generation proteomics: towards an integrative view of proteome dynamics
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TLDR
MS-based proteomics is starting to mature and to deliver through a combination of developments in instrumentation, sample preparation and computational analysis, and to highlight recent applications.Abstract:
Next-generation sequencing allows the analysis of genomes, including those representing disease states. However, the causes of most disorders are multifactorial, and systems-level approaches, including the analysis of proteomes, are required for a more comprehensive understanding. The proteome is extremely multifaceted owing to splicing and protein modifications, and this is further amplified by the interconnectivity of proteins into complexes and signalling networks that are highly divergent in time and space. Proteome analysis heavily relies on mass spectrometry (MS). MS-based proteomics is starting to mature and to deliver through a combination of developments in instrumentation, sample preparation and computational analysis. Here we describe this emerging next generation of proteomics and highlight recent applications.read more
Citations
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Identification of proteins that mediate the role of androgens in antler regeneration using label free proteomics in sika deer (Cervus nippon).
TL;DR: The results suggest that up-regulated heat shock proteins, peptidyl-prolyl cis-trans isomerase 4, mitogen-activated protein kinase 3, MAPK3 and calreticulin and down-regulated SHC-transforming protein 1, heat shock protein family A member 1A (HSPA1A) and proto-oncogene tyrosine-protein kinase (SRC) may be associated directly or indirectly with antler regeneration.
Journal ArticleDOI
Highly Sensitive and Multiplexed Protein Imaging With Cleavable Fluorescent Tyramide Reveals Human Neuronal Heterogeneity.
Renjie Liao,Manas Mondal,Christopher D. Nazaroff,Christopher D. Nazaroff,Diego Mastroeni,Paul D. Coleman,Joshua LaBaer,Jia Guo +7 more
TL;DR: In this paper, a highly sensitive and multiplexed in situ protein analysis approach using cleavable fluorescent tyramide and off-the-shelf antibodies was presented, which can detect hundreds of proteins in intact tissues at the optical resolution.
Journal ArticleDOI
Proteomic Reveals Reasons for Acquired Drug Resistance in Lung Cancer Derived Brain Metastasis Based on a Newly Established Multi-Organ Microfluidic Chip Model
TL;DR: In this article, a preclinical model based on a multi-organ microfluidic chip has been established proficiently in previous work, and the BM subpopulation derived from the parental PC9 cell line was isolated from the chip model and found to develop obvious resistance to antineoplastic drugs including chemotherapeutic agents (cisplatin, carboplatin, pemetrexed) and tyrosine kinase inhibitors (TKIs) which target epidermal growth factor receptor (EGFR).
Journal ArticleDOI
MS-DAP Platform for Downstream Data Analysis of Label-Free Proteomics Uncovers Optimal Workflows in Benchmark Data Sets and Increased Sensitivity in Analysis of Alzheimer’s Biomarker Data
TL;DR: MS-DAP as discussed by the authors is an open-source platform for data normalization and statistical analysis of CSF from AD patients that integrates both popular and recently developed algorithms for normalisation and statistical analyses.
Journal ArticleDOI
Emerging roles of non-histone protein crotonylation in biomedicine.
TL;DR: In this paper, the authors summarized the main proteomic characteristics of non-histone protein Kcr and discussed its biological functions, including gene transcription, DNA damage response, enzymes regulation, metabolic pathways, cell cycle, and localization of heterochromatin in cells.
References
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