Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas system
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TLDR
The CRISPR/Cas system allows targeted cleavage of genomic DNA guided by a customizable small noncoding RNA, resulting in gene modifications by both non-homologous end joining (NHEJ) and homology-directed repair (HDR) mechanisms.Abstract:
Targeted genome engineering (also known as genome editing) has emerged as an alternative to classical plant breeding and transgenic (GMO) methods to improve crop plants. Until recently, available tools for introducing site-specific double strand DNA breaks were restricted to zinc finger nucleases (ZFNs) and TAL effector nucleases (TALENs). However, these technologies have not been widely adopted by the plant research community due to complicated design and laborious assembly of specific DNA binding proteins for each target gene. Recently, an easier method has emerged based on the bacterial type II CRISPR (clustered regularly interspaced short palindromic repeats)/Cas (CRISPR-associated) immune system. The CRISPR/Cas system allows targeted cleavage of genomic DNA guided by a customizable small noncoding RNA, resulting in gene modifications by both non-homologous end joining (NHEJ) and homology-directed repair (HDR) mechanisms. In this review we summarize and discuss recent applications of the CRISPR/Cas technology in plants.read more
Citations
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Journal ArticleDOI
Multifaceted regulatory function of tomato SlTAF1 in the response to salinity stress
Vikas Devkar,Vikas Devkar,Venkatesh P Thirumalaikumar,Venkatesh P Thirumalaikumar,Gang-Ping Xue,José G. Vallarino,Veronika Turečková,Miroslav Strnad,Alisdair R. Fernie,Rainer Hoefgen,Bernd Mueller-Roeber,Bernd Mueller-Roeber,Salma Balazadeh,Salma Balazadeh +13 more
TL;DR: SlTAF1 controls tomato's response to salinity stress by combating both, osmotic and ion toxicity, highlighting it as a promising candidate for the future breeding of stress-tolerant crops.
Journal ArticleDOI
CRISPR-Local: a local single-guide RNA (sgRNA) design tool for non-reference plant genomes.
Jiamin Sun,Hao Liu,Jianxiao Liu,Shikun Cheng,Yong Peng,Qinghua Zhang,Jianbing Yan,Haijun Liu,Ling-Ling Chen +8 more
TL;DR: CRISPR-Local outperforms other sgRNA design tools in the following respects: designing sg RNAs suitable for non-reference varieties; screening for sgRNAs that are capable of simultaneously targeting multiple genes; saving computational resources by avoiding repeated calculations from multiple submissions; and running offline, with both command-line and graphical user interface modes.
Journal ArticleDOI
CsLFY is required for shoot meristem maintenance via interaction with WUSCHEL in cucumber (Cucumis sativus)
Wensheng Zhao,Zijing Chen,Xiaofeng Liu,Gen Che,Ran Gu,Jianyu Zhao,Zhongyi Wang,Yu Hou,Xiaolan Zhang +8 more
TL;DR: It is suggested that CsLFY has a novel function in regulating shoot meristem maintenance through interaction with CsWUS, and promotes flower development via activation of CsAP3 and CUM1 in cucumber.
Patent
Compositions and methods for producing plants resistant to glyphosate herbicide
TL;DR: In this paper, a guide polynucleotide/Cas endonuclease system was used to identify and insert a double strand break at a specific target site into the genome of a cell, where the nucleotide sequence of interest to be edited can be located within or outside the target site that is recognized by a Cas end-uclease.
Journal ArticleDOI
Hotter, drier, CRISPR: the latest edit on climate change
TL;DR: In this article, the integration of genome editing and transgenics into current breeding strategies is one promising solution to accelerate genetic gains through targeted genetic modifications, producing crops that can overcome the shifting climate realities.
References
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