Pyruvate Kinase Isoform Expression Alters Nucleotide Synthesis to Impact Cell Proliferation
Sophia Y. Lunt,Sophia Y. Lunt,Vinayak Muralidhar,Vinayak Muralidhar,Aaron M. Hosios,William J. Israelsen,Dan Y. Gui,Lauren Newhouse,Martin P. Ogrodzinski,Vivian C. Hecht,Kali Xu,Paula N. Marin Acevedo,Daniel P. Hollern,Gary Bellinger,Talya L. Dayton,Stefan Christen,Ilaria Elia,Anh T. Dinh,Gregory Stephanopoulos,Scott R. Manalis,Michael B. Yaffe,Eran R. Andrechek,Sarah-Maria Fendt,Matthew G. Vander Heiden,Matthew G. Vander Heiden +24 more
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TLDR
In this article, the authors examined how PKM2 deletion affects proliferation and metabolism in nontransformed, non-immortalized PKM-2-expressing primary cells and found that deletion in primary cells results in PKM1 expression and proliferation arrest.About:
This article is published in Molecular Cell.The article was published on 2015-01-08 and is currently open access. It has received 213 citations till now. The article focuses on the topics: Cellular differentiation & Cell growth.read more
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Understanding the Intersections between Metabolism and Cancer Biology
TL;DR: In this paper, the authors define pathways that are limiting for cancer progression and understand the context specificity of metabolic preferences and liabilities in malignant cells, which can guide the more effective targeting of metabolism to help patients.
Journal ArticleDOI
Supporting Aspartate Biosynthesis Is an Essential Function of Respiration in Proliferating Cells
Lucas B. Sullivan,Dan Y. Gui,Aaron M. Hosios,Lauren N. Bush,Elizaveta Freinkman,Matthew G. Vander Heiden,Matthew G. Vander Heiden +6 more
TL;DR: It is found that electron acceptors are limiting for producing aspartates, and supplying aspartate enables proliferation of respiration deficient cells in the absence of exogenous electron acceptor.
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Genetic compensation: A phenomenon in search of mechanisms.
TL;DR: This review revisits studies reporting genetic compensation in higher eukaryotes and outlines possible molecular mechanisms, which may include both transcriptional and posttranscriptional processes.
Journal ArticleDOI
A roadmap for interpreting 13 C metabolite labeling patterns from cells
Joerg Martin Buescher,Maciek R. Antoniewicz,Laszlo G. Boros,Shawn C. Burgess,Henri Brunengraber,Clary B. Clish,Ralph J. DeBerardinis,Olivier Feron,Christian Frezza,Bart Ghesquière,Eyal Gottlieb,Karsten Hiller,Russell G. Jones,Jurre J. Kamphorst,Richard G. Kibbey,Alec C. Kimmelman,Jason W. Locasale,Sophia Y. Lunt,Oliver D. K. Maddocks,Craig R. Malloy,Christian M. Metallo,Emmanuelle J. Meuillet,Joshua Munger,Katharina Nöh,Joshua D. Rabinowitz,Markus Ralser,Markus Ralser,Uwe Sauer,Gregory Stephanopoulos,Julie St-Pierre,Daniel A. Tennant,Christoph Wittmann,Matthew G. Vander Heiden,Matthew G. Vander Heiden,Alexei Vazquez,Karen H. Vousden,Jamey D. Young,Nicola Zamboni,Sarah-Maria Fendt +38 more
TL;DR: Key issues in interpreting (13)C metabolite labeling patterns are reviewed, with the goal of drawing accurate conclusions from steady state and dynamic stable isotopic tracer experiments.
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Fatty acid carbon is essential for dNTP synthesis in endothelial cells
Sandra Schoors,Ulrike Bruning,Rindert Missiaen,Karla C. S. Queiroz,Gitte Borgers,Ilaria Elia,Annalisa Zecchin,Anna Rita Cantelmo,Stefan Christen,Jermaine Goveia,Ward Heggermont,Lucica Godde,Stefan Vinckier,Paul P. Van Veldhoven,Guy Eelen,Luc Schoonjans,Holger Gerhardt,Mieke Dewerchin,Myriam Baes,Katrien De Bock,Bart Ghesquière,Sophia Y. Lunt,Sarah-Maria Fendt,Peter Carmeliet +23 more
TL;DR: It is reported that endothelial loss of CPT1A, a rate-limiting enzyme of fatty acid oxidation (FAO), causes vascular sprouting defects due to impaired proliferation, not migration, of human and murine endothelial cells.
References
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A biomarker that identifies senescent human cells in culture and in aging skin in vivo
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