Regeneration and experimental orthotopic transplantation of a bioengineered kidney
Jeremy Song,Jacques P. Guyette,Sarah E. Gilpin,Gabriel Gonzalez,Joseph P. Vacanti,Harald C. Ott +5 more
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TLDR
To regenerate functional tissue, rat kidney scaffolds are seeded with epithelial and endothelial cells and perfused these cell-seeded constructs in a whole-organ bioreactor, resulting in grafts that produced rudimentary urine in vitro when perfused through their intrinsic vascular bed.Abstract:
Approximately 100,000 individuals in the United States currently await kidney transplantation, and 400,000 individuals live with end-stage kidney disease requiring hemodialysis. The creation of a transplantable graft to permanently replace kidney function would address donor organ shortage and the morbidity associated with immunosuppression. Such a bioengineered graft must have the kidney's architecture and function and permit perfusion, filtration, secretion, absorption and drainage of urine. We decellularized rat, porcine and human kidneys by detergent perfusion, yielding acellular scaffolds with vascular, cortical and medullary architecture, a collecting system and ureters. To regenerate functional tissue, we seeded rat kidney scaffolds with epithelial and endothelial cells and perfused these cell-seeded constructs in a whole-organ bioreactor. The resulting grafts produced rudimentary urine in vitro when perfused through their intrinsic vascular bed. When transplanted in an orthotopic position in rat, the grafts were perfused by the recipient's circulation and produced urine through the ureteral conduit in vivo.read more
Citations
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Regenerative medicine in kidney disease: where we stand and where to go.
Fernanda Borges,Nestor Schor +1 more
TL;DR: New mechanisms studied for kidney regeneration and repair include circulating stem cells as mesenchymal stromal/stem cells and their paracrine mechanisms of action; renal progenitor stem cells; the leading role of tubular epithelial cells in the tubular repair process; the study of zebrafish larvae; and, finally, the development of organoids.
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Decellularized adipose matrix provides an inductive microenvironment for stem cells in tissue regeneration
Jizhong Yang,Lihong Qiu,Shaoheng Xiong,Juanli Dang,Xiang-Ke Rong,Hou Mengmeng,Kai Wang,Zhou Yu,Chenggang Yi +8 more
TL;DR: The methods for decellularizing and sterilizing adipose tissue, and the impact of these methods on the biological and physical properties of DAM are summarized.
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Recent advancement of decellularization extracellular matrix for tissue engineering and biomedical application.
Jiamin Yang,Hangyu Dang,Yi Xu +2 more
TL;DR: Decellularized extracellular matrix (dECM) derived from organs and tissues have emerged as a promising tool, as they encompass the characteristics of an ideal tissue scaffold: complex composition, vascular networks and unique tissue-specific architecture as mentioned in this paper.
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The angiogenesis in decellularized scaffold-mediated the renal regeneration.
Jin Mei,Yaling Yu,Miaozhong Li,Shanshan Xi,Sixiao Zhang,Xiaolin Liu,Junqun Jiang,Zhibin Wang,Jianse Zhang,Yu-Qiang Ding,Xin-fa Lou,Maolin Tang +11 more
TL;DR: The data indicate that decellularized (DC) scaffold can be vascularized in vivo and possible mechanisms are discussed, which could improve disturbed renal function after partial nephrectomy.
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Kidney transplantation, bioengineering and regeneration: an originally immunology-based discipline destined to transition towards ad hoc organ manufacturing and repair
Jeffrey Rogers,Ravi Katari,Sheyna Gifford,Riccardo Tamburrini,Lauren Edgar,Marcia Voigt,Sean V. Murphy,Daniel Igel,Sara Mancone,Tyler E. Callese,Nicola Colucci,Majid Mirzazadeh,Andrea Peloso,Joao Paulo Zambon,Alan C. Farney,Robert J. Stratta,Giuseppe Orlando +16 more
TL;DR: The current status of renal transplantation is discussed while reviewing the most promising innovations in RM therapy as applied to RRT, highlighting the potential to shift the paradigm in RRT through organ bioengineering.
References
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Perfusion-decellularized matrix: using nature's platform to engineer a bioartificial heart
Harald C. Ott,Thomas S Matthiesen,Saik Kia Goh,Lauren D. Black,Stefan M. Kren,Theoden I. Netoff,Doris A. Taylor +6 more
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Regeneration and orthotopic transplantation of a bioartificial lung
Harald C. Ott,Ben Clippinger,Claudius Conrad,Christian Schuetz,Irina Pomerantseva,Laertis Ikonomou,Darrell N. Kotton,Joseph P. Vacanti +7 more
TL;DR: Creation of a bioartificial lung requires engineering of viable lung architecture enabling ventilation, perfusion and gas exchange, and regenerated lungs into orthotopic position showed in vivo function.
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HLA-Mismatched Renal Transplantation without Maintenance Immunosuppression
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TL;DR: Five patients with end-stage renal disease received combined bone marrow and kidney transplants from HLA single-haplotype mismatched living related donors, with the use of a nonmyeloablative preparative regimen, and it was possible to discontinue all immunosuppressive therapy 9 to 14 months after the transplantation.
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Organ engineering based on decellularized matrix scaffolds
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