Regeneration and experimental orthotopic transplantation of a bioengineered kidney
Jeremy Song,Jacques P. Guyette,Sarah E. Gilpin,Gabriel Gonzalez,Joseph P. Vacanti,Harald C. Ott +5 more
Reads0
Chats0
TLDR
To regenerate functional tissue, rat kidney scaffolds are seeded with epithelial and endothelial cells and perfused these cell-seeded constructs in a whole-organ bioreactor, resulting in grafts that produced rudimentary urine in vitro when perfused through their intrinsic vascular bed.Abstract:
Approximately 100,000 individuals in the United States currently await kidney transplantation, and 400,000 individuals live with end-stage kidney disease requiring hemodialysis. The creation of a transplantable graft to permanently replace kidney function would address donor organ shortage and the morbidity associated with immunosuppression. Such a bioengineered graft must have the kidney's architecture and function and permit perfusion, filtration, secretion, absorption and drainage of urine. We decellularized rat, porcine and human kidneys by detergent perfusion, yielding acellular scaffolds with vascular, cortical and medullary architecture, a collecting system and ureters. To regenerate functional tissue, we seeded rat kidney scaffolds with epithelial and endothelial cells and perfused these cell-seeded constructs in a whole-organ bioreactor. The resulting grafts produced rudimentary urine in vitro when perfused through their intrinsic vascular bed. When transplanted in an orthotopic position in rat, the grafts were perfused by the recipient's circulation and produced urine through the ureteral conduit in vivo.read more
Citations
More filters
Journal ArticleDOI
Current achievements and future perspectives in whole-organ bioengineering.
Andrea Peloso,Andrea Peloso,Abritee Dhal,Joao Paulo Zambon,Peng Li,Peng Li,Giuseppe Orlando,Giuseppe Orlando,Anthony Atala,Anthony Atala,Shay Soker +10 more
TL;DR: This review will emphasize recent achievements in the whole-organ scaffolds and underline complications that the scientific community has to resolve before reaching a functional bioengineered organ.
Journal ArticleDOI
Tissue-engineered kidney disease models.
TL;DR: Tissue engineered models of polycystic kidney disease, drug-induced nephrotoxicity, and the glomerulus have the capacity to compensate for the limitations of traditional modalities.
Journal ArticleDOI
Rapid production of human liver scaffolds for functional tissue engineering by high shear stress oscillation-decellularization
Giuseppe Mazza,Walid Al-Akkad,Andrea Telese,Lisa Longato,Luca Urbani,Benjamin Robinson,Andrew M. Hall,Kenny Kong,L. Frenguelli,Giusi Marrone,Oliver Willacy,Mohsen Shaeri,Alan J. Burns,Alan J. Burns,Massimo Malagó,Janet A. Gilbertson,Nigel B. Rendell,Kevin Moore,David Hughes,Ioan Notingher,Gavin Jell,Armando del Río Hernández,Paolo De Coppi,Krista Rombouts,Massimo Pinzani +24 more
TL;DR: The design and validation of a new methodology for the rapid and accurate production of human acellular liver tissue cubes (ALTCs) using normal liver tissue unsuitable for transplantation is reported and provides a solid basis for the establishment of effective protocols aimed at recreating human liver tissue in vitro.
Journal ArticleDOI
Renal Extracellular Matrix Scaffolds From Discarded Kidneys Maintain Glomerular Morphometry and Vascular Resilience and Retains Critical Growth Factors.
Andrea Peloso,Astgik Petrosyan,Stefano Da Sacco,Christopher Booth,Joao Paulo Zambon,Timothy OʼBrien,Charles Aardema,John L. Robertson,Roger E. De Filippo,Shay Soker,Robert J. Stratta,Laura Perin,Giuseppe Orlando +12 more
TL;DR: The results indicate that discarded human kidneys are a suitable source of renal scaffolds because they maintain a well-preserved structure and function of the vasculature, as well as GFs that are fundamental to achieve a satisfying recellularization of the scaffold in vivo due to their angiogenic properties.
Journal ArticleDOI
Direct reprogramming of fibroblasts into renal tubular epithelial cells by defined transcription factors.
Michael M. Kaminski,Jelena Tosic,Catena Kresbach,Hannes Engel,Jonas Klockenbusch,Anna-Lena Müller,Roman Pichler,Florian Grahammer,Oliver Kretz,Tobias B. Huber,Gerd Walz,Sebastian J. Arnold,Soeren S. Lienkamp +12 more
TL;DR: This approach demonstrates that reprogramming factors can be identified by targeted in silico analysis and converts mouse and human fibroblasts into induced renal tubular epithelial cells (iRECs), and identifies renal cell fate-inducing factors on the basis of their tissue specificity and evolutionarily conserved expression.
References
More filters
Journal ArticleDOI
Perfusion-decellularized matrix: using nature's platform to engineer a bioartificial heart
Harald C. Ott,Thomas S Matthiesen,Saik Kia Goh,Lauren D. Black,Stefan M. Kren,Theoden I. Netoff,Doris A. Taylor +6 more
TL;DR: Eight constructs decellularized hearts by coronary perfusion with detergents, preserved the underlying extracellular matrix, and produced an acellular, perfusable vascular architecture, competent a cellular valves and intact chamber geometry that could generate pump function in a modified working heart preparation.
Journal ArticleDOI
Tissue-engineered autologous bladders for patients needing cystoplasty
TL;DR: Engineered bladder tissues, created with autologous cells seeded on collagen-polyglycolic acid scaffolds, and wrapped in omentum after implantation, can be used in patients who need cystoplasty.
Journal ArticleDOI
Regeneration and orthotopic transplantation of a bioartificial lung
Harald C. Ott,Ben Clippinger,Claudius Conrad,Christian Schuetz,Irina Pomerantseva,Laertis Ikonomou,Darrell N. Kotton,Joseph P. Vacanti +7 more
TL;DR: Creation of a bioartificial lung requires engineering of viable lung architecture enabling ventilation, perfusion and gas exchange, and regenerated lungs into orthotopic position showed in vivo function.
Journal ArticleDOI
HLA-Mismatched Renal Transplantation without Maintenance Immunosuppression
Tatsuo Kawai,A. Benedict Cosimi,Thomas R. Spitzer,Nina Tolkoff-Rubin,Manikkam Suthanthiran,Susan L. Saidman,Juanita Shaffer,Frederic I. Preffer,Ruchuang Ding,Vijay K. Sharma,Jay A. Fishman,Bimalangshu R. Dey,Dicken S.C. Ko,Martin Hertl,Nelson Goes,Waichi Wong,Winfred W. Williams,Robert B. Colvin,Megan Sykes,David H. Sachs +19 more
TL;DR: Five patients with end-stage renal disease received combined bone marrow and kidney transplants from HLA single-haplotype mismatched living related donors, with the use of a nonmyeloablative preparative regimen, and it was possible to discontinue all immunosuppressive therapy 9 to 14 months after the transplantation.
Journal ArticleDOI
Organ engineering based on decellularized matrix scaffolds
Jeremy Song,Harald C. Ott +1 more
TL;DR: A review summarizes achievements to date and discusses the role of native ECM scaffolds in organ regeneration, which provides a promising alternative to synthetic scaffolds and a foundation for regenerative efforts.
Related Papers (5)
Organ reengineering through development of a transplantable recellularized liver graft using decellularized liver matrix
Basak E. Uygun,Alejandro Soto-Gutierrez,Alejandro Soto-Gutierrez,Hiroshi Yagi,Hiroshi Yagi,Maria Louisa Izamis,Maria Angela Guzzardi,Maria Angela Guzzardi,Carley Shulman,Jack M. Milwid,Naoya Kobayashi,Arno W. Tilles,Francois Berthiaume,Francois Berthiaume,Martin Hertl,Yaakov Nahmias,Yaakov Nahmias,Martin L. Yarmush,Martin L. Yarmush,Korkut Uygun +19 more