Regeneration and experimental orthotopic transplantation of a bioengineered kidney
Jeremy Song,Jacques P. Guyette,Sarah E. Gilpin,Gabriel Gonzalez,Joseph P. Vacanti,Harald C. Ott +5 more
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TLDR
To regenerate functional tissue, rat kidney scaffolds are seeded with epithelial and endothelial cells and perfused these cell-seeded constructs in a whole-organ bioreactor, resulting in grafts that produced rudimentary urine in vitro when perfused through their intrinsic vascular bed.Abstract:
Approximately 100,000 individuals in the United States currently await kidney transplantation, and 400,000 individuals live with end-stage kidney disease requiring hemodialysis. The creation of a transplantable graft to permanently replace kidney function would address donor organ shortage and the morbidity associated with immunosuppression. Such a bioengineered graft must have the kidney's architecture and function and permit perfusion, filtration, secretion, absorption and drainage of urine. We decellularized rat, porcine and human kidneys by detergent perfusion, yielding acellular scaffolds with vascular, cortical and medullary architecture, a collecting system and ureters. To regenerate functional tissue, we seeded rat kidney scaffolds with epithelial and endothelial cells and perfused these cell-seeded constructs in a whole-organ bioreactor. The resulting grafts produced rudimentary urine in vitro when perfused through their intrinsic vascular bed. When transplanted in an orthotopic position in rat, the grafts were perfused by the recipient's circulation and produced urine through the ureteral conduit in vivo.read more
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End-stage renal disease: A bioengineered kidney with excretory function
TL;DR: The regenerated kidneys were capable of excretory function during perfusion in vitro and after orthotopic transplantation in vivo and the researchers believe these findings might correspond to the relative immaturity of the constructs and hope that further maturation will improve vascular patency and electrolyte re absorption.
Journal ArticleDOI
Patterned vascularization in a directional ice-templated scaffold of decellularized matrix
Li Shen,Xiuyue Song,Yalan Xu,Runhua Tian,Yin Wang,Peifeng Li,Jing Li,Hao Bai,Hai Zhu,Dong Wang +9 more
TL;DR: This method for patterning microvessels in a directional ice‐templated scaffold of decellularized porcine kidney extracellular matrix without any fixatives or cross‐linkers maximized the potential of tissue remodeling.
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Intermittent application of external positive pressure helps to preserve organ viability during ex vivo perfusion and culture
TL;DR: In this article, a bioreactor system was used to perfuse and culture rat small intestine and femoral muscle preparations and the authors evaluated whether intermittent external pressure would improve the perfusion and viability of organs in culture.
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Designer organs: The future of personalized transplantation.
TL;DR: In this review, the recent progress using gene knockout and knock-in strategies used mainly in the context of xenotransplantation are highlighted and advancements in CRISPR-Cas9 gene editing and RNA interference in relation to organ transplantation are discussed.
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Considerations for the clinical use of stem cells in genitourinary regenerative medicine.
TL;DR: In this article, a review of the recent advances using these approaches are described, with a focus on stromal/mesenchymal cells found in adipose tissue, and an innovative technique allowing tissue reconstruction without exogenous material, the selfassembly approach, is presented.
References
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Perfusion-decellularized matrix: using nature's platform to engineer a bioartificial heart
Harald C. Ott,Thomas S Matthiesen,Saik Kia Goh,Lauren D. Black,Stefan M. Kren,Theoden I. Netoff,Doris A. Taylor +6 more
TL;DR: Eight constructs decellularized hearts by coronary perfusion with detergents, preserved the underlying extracellular matrix, and produced an acellular, perfusable vascular architecture, competent a cellular valves and intact chamber geometry that could generate pump function in a modified working heart preparation.
Journal ArticleDOI
Tissue-engineered autologous bladders for patients needing cystoplasty
TL;DR: Engineered bladder tissues, created with autologous cells seeded on collagen-polyglycolic acid scaffolds, and wrapped in omentum after implantation, can be used in patients who need cystoplasty.
Journal ArticleDOI
Regeneration and orthotopic transplantation of a bioartificial lung
Harald C. Ott,Ben Clippinger,Claudius Conrad,Christian Schuetz,Irina Pomerantseva,Laertis Ikonomou,Darrell N. Kotton,Joseph P. Vacanti +7 more
TL;DR: Creation of a bioartificial lung requires engineering of viable lung architecture enabling ventilation, perfusion and gas exchange, and regenerated lungs into orthotopic position showed in vivo function.
Journal ArticleDOI
HLA-Mismatched Renal Transplantation without Maintenance Immunosuppression
Tatsuo Kawai,A. Benedict Cosimi,Thomas R. Spitzer,Nina Tolkoff-Rubin,Manikkam Suthanthiran,Susan L. Saidman,Juanita Shaffer,Frederic I. Preffer,Ruchuang Ding,Vijay K. Sharma,Jay A. Fishman,Bimalangshu R. Dey,Dicken S.C. Ko,Martin Hertl,Nelson Goes,Waichi Wong,Winfred W. Williams,Robert B. Colvin,Megan Sykes,David H. Sachs +19 more
TL;DR: Five patients with end-stage renal disease received combined bone marrow and kidney transplants from HLA single-haplotype mismatched living related donors, with the use of a nonmyeloablative preparative regimen, and it was possible to discontinue all immunosuppressive therapy 9 to 14 months after the transplantation.
Journal ArticleDOI
Organ engineering based on decellularized matrix scaffolds
Jeremy Song,Harald C. Ott +1 more
TL;DR: A review summarizes achievements to date and discusses the role of native ECM scaffolds in organ regeneration, which provides a promising alternative to synthetic scaffolds and a foundation for regenerative efforts.
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