Regeneration and experimental orthotopic transplantation of a bioengineered kidney
Jeremy Song,Jacques P. Guyette,Sarah E. Gilpin,Gabriel Gonzalez,Joseph P. Vacanti,Harald C. Ott +5 more
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TLDR
To regenerate functional tissue, rat kidney scaffolds are seeded with epithelial and endothelial cells and perfused these cell-seeded constructs in a whole-organ bioreactor, resulting in grafts that produced rudimentary urine in vitro when perfused through their intrinsic vascular bed.Abstract:
Approximately 100,000 individuals in the United States currently await kidney transplantation, and 400,000 individuals live with end-stage kidney disease requiring hemodialysis. The creation of a transplantable graft to permanently replace kidney function would address donor organ shortage and the morbidity associated with immunosuppression. Such a bioengineered graft must have the kidney's architecture and function and permit perfusion, filtration, secretion, absorption and drainage of urine. We decellularized rat, porcine and human kidneys by detergent perfusion, yielding acellular scaffolds with vascular, cortical and medullary architecture, a collecting system and ureters. To regenerate functional tissue, we seeded rat kidney scaffolds with epithelial and endothelial cells and perfused these cell-seeded constructs in a whole-organ bioreactor. The resulting grafts produced rudimentary urine in vitro when perfused through their intrinsic vascular bed. When transplanted in an orthotopic position in rat, the grafts were perfused by the recipient's circulation and produced urine through the ureteral conduit in vivo.read more
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Reconstruction of functional uterine tissues through recellularizing the decellularized rat uterine scaffolds by MSCs in vivo and in vitro
Xia Li,Yiming Wang,Ruoyu Ma,Xin Liu,Biaobiao Song,Yongchao Duan,Jia Guo,Guihai Feng,Tongtong Cui,Liu Wang,Jie Hao,Hongmei Wang,Qi Gu +12 more
TL;DR: A well-defined system to regenerate a functional rat uterine through recellularization of the decellularized uterine matrix (DUM) patches reseeded with human mesenchymal stem cells (hMSCs) enhanced the regeneration of traumatic uterine in vivo because of MSC regulation.
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Metabolic glycan labeling and chemoselective functionalization of native biomaterials.
Xi Ren,Daniele Evangelista-Leite,Tong Wu,Taufiek Konrad Rajab,Philipp T. Moser,Kentaro Kitano,Konstantinos P. Economopoulos,Daniel Gorman,Jordan P. Bloom,Jun Jie Tan,Sarah E. Gilpin,Haiyang Zhou,Douglas J. Mathisen,Harald C. Ott +13 more
TL;DR: The herein reported metabolic glycan labeling approach represents a novel platform technology for manufacturing click-reactive native ECM biomaterials, thereby enabling efficient and chemoselective functionalization of these materials to facilitate tissue regeneration and repair.
Journal ArticleDOI
Adult stem-like cells in kidney.
Keiichi Hishikawa,Osamu Takase,Masahiro Yoshikawa,Taro Tsujimura,Masaomi Nangaku,Tsuyoshi Takato +5 more
TL;DR: The recent broad findings of kidney regeneration and the cells that contribute regeneration are described, suggesting that adult stem-like cells in kidney can be practical clinical targets for kidney diseases.
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Spray Delivery of Intestinal Organoids to Reconstitute Epithelium on Decellularized Native Extracellular Matrix.
Dana M. Schwartz,Meryem O. Pehlivaner Kara,Allan M. Goldstein,Harald C. Ott,Adam K. Ekenseair +4 more
TL;DR: The native extracellular matrix (ECM) serves as a unique platform for tissue engineering because it provides an organ-specific scaffold in terms of both matrix composition and tissue architecture.
Journal ArticleDOI
Hepatic cell-sheet fabrication of differentiated mesenchymal stem cells using decellularized extracellular matrix and thermoresponsive polymer.
Maryam Asadi,Hajie Lotfi,Roya Salehi,Ahmad Mehdipour,Nosratollah Zarghami,Abolfazl Akbarzadeh,Effat Alizadeh +6 more
TL;DR: In this article, three in vitro microenvironments models including I: pNIPAAm hydrogel, II: decellularized extracellular matrix (ECM) incorporated into pNIPPAAm, and III: dECM scaffold were generated to investigate the structural and function cues of hepatocyte-like cells after differentiation of mesenchymal stem cells.
References
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Perfusion-decellularized matrix: using nature's platform to engineer a bioartificial heart
Harald C. Ott,Thomas S Matthiesen,Saik Kia Goh,Lauren D. Black,Stefan M. Kren,Theoden I. Netoff,Doris A. Taylor +6 more
TL;DR: Eight constructs decellularized hearts by coronary perfusion with detergents, preserved the underlying extracellular matrix, and produced an acellular, perfusable vascular architecture, competent a cellular valves and intact chamber geometry that could generate pump function in a modified working heart preparation.
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Tissue-engineered autologous bladders for patients needing cystoplasty
TL;DR: Engineered bladder tissues, created with autologous cells seeded on collagen-polyglycolic acid scaffolds, and wrapped in omentum after implantation, can be used in patients who need cystoplasty.
Journal ArticleDOI
Regeneration and orthotopic transplantation of a bioartificial lung
Harald C. Ott,Ben Clippinger,Claudius Conrad,Christian Schuetz,Irina Pomerantseva,Laertis Ikonomou,Darrell N. Kotton,Joseph P. Vacanti +7 more
TL;DR: Creation of a bioartificial lung requires engineering of viable lung architecture enabling ventilation, perfusion and gas exchange, and regenerated lungs into orthotopic position showed in vivo function.
Journal ArticleDOI
HLA-Mismatched Renal Transplantation without Maintenance Immunosuppression
Tatsuo Kawai,A. Benedict Cosimi,Thomas R. Spitzer,Nina Tolkoff-Rubin,Manikkam Suthanthiran,Susan L. Saidman,Juanita Shaffer,Frederic I. Preffer,Ruchuang Ding,Vijay K. Sharma,Jay A. Fishman,Bimalangshu R. Dey,Dicken S.C. Ko,Martin Hertl,Nelson Goes,Waichi Wong,Winfred W. Williams,Robert B. Colvin,Megan Sykes,David H. Sachs +19 more
TL;DR: Five patients with end-stage renal disease received combined bone marrow and kidney transplants from HLA single-haplotype mismatched living related donors, with the use of a nonmyeloablative preparative regimen, and it was possible to discontinue all immunosuppressive therapy 9 to 14 months after the transplantation.
Journal ArticleDOI
Organ engineering based on decellularized matrix scaffolds
Jeremy Song,Harald C. Ott +1 more
TL;DR: A review summarizes achievements to date and discusses the role of native ECM scaffolds in organ regeneration, which provides a promising alternative to synthetic scaffolds and a foundation for regenerative efforts.
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